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41

GATE BT 2022 | Question: 55
A bacterial strain is grown in nutrient medium at $37^{\circ}\text{C}$ under aerobic conditions. The medium is inoculated with $10^{2}$ cells from a seed culture. If the number of cells in the culture is $10^{5}$ after $10$ hours of growth, the doubling time of the strain (rounded off to nearest integer) is ___________ $\text{h}.$

A bacterial strain is grown in nutrient medium at $37^{\circ}\text{C}$ under aerobic conditions. The medium is inoculated with $10^{2}$ cells from a seed culture. If the ...

Lakshman Bhaiya

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Lakshman Bhaiya asked Mar 20, 2022

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42

GATE2019: 53
In a cross-flow filtration process, the pressure drop $\left ( \Delta P \right )$ driving the fluid flow is $2$ atm, inlet feed pressure $\left ( P_{i} \right )$ is $3$ atm and filtrate pressure $\left ( P_{f} \right )$ is equal to atmospheric pressure. The average transmembrane pressure drop $\left ( \Delta P_{m} \right )$ is ___________ atm.

In a cross-flow filtration process, the pressure drop $\left ( \Delta P \right )$ driving the fluid flow is $2$ atm, inlet feed pressure $\left ( P_{i} \right )$ is $3$ a...

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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43

GATE2013-46
The maximum cell concentration $(\text{g l}^{-1})$ expected in a bioreactor with initial cell concentration of $\text{1.75 g l}^{-1}$ and an initial glucose concentration of $\text{125 g l}^{-1}$ is ($Y_{X/S} = 0.6\:g\:cell/g$ substrate) ____________.

The maximum cell concentration $(\text{g l}^{-1})$ expected in a bioreactor with initial cell concentration of $\text{1.75 g l}^{-1}$ and an initial glucose concentration...

Milicevic3306

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Milicevic3306 asked Mar 25, 2018

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44

GATE2018-47
In a chemostat, the feed flow rate and culture volume are $100$ ml/h and $1.0$ L, respectively. With glucose as substrate, the values of $\mu_{\text{max}}$ and $K_s$ are $0.2 \: h^{-1}$ and $1 \: g/L$, respectively. For a glucose concentration of $10 \: g/L$ in the feed, the effluent substrate concentration (in $g/L$) is _______

In a chemostat, the feed flow rate and culture volume are $100$ ml/h and $1.0$ L, respectively. With glucose as substrate, the values of $\mu_{\text{max}}$ and $K_s$ are ...

gatecse

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gatecse asked Feb 20, 2018

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45

GATE BT 2023 | Question: 10
The correct sequence of events during bacteriophage infection of a bacterial cell is landing $\rightarrow$ attachment $\rightarrow$ tail contraction $\rightarrow$ penetration and unplugging $\rightarrow$ DNA ejection attachment $\rightarrow$ ... $\rightarrow$ tail contraction $\rightarrow$ landing $\rightarrow$ penetration and unplugging $\rightarrow$ DNA ejection

The correct sequence of events during bacteriophage infection of a bacterial cell islanding $\rightarrow$ attachment $\rightarrow$ tail contraction $\rightarrow$ penetrat...

admin

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admin asked May 21, 2023

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46

GATE BT 2023 | Question: 3
Determine the correctness or otherwise of the following Assertion $[\text{a}]$ and the Reason $[\mathrm{r}]$. Assertion $[\mathrm{a}]$: In multicellular organisms, cells of different lineages have different gene expression profiles. Reason $[\mathrm{r}]$: Alternative splicing ... not the correct reason for $[\mathrm{a}]$ $[\mathrm{a}]$ is true but $[\mathrm{r}]$ is false

Determine the correctness or otherwise of the following Assertion $[\text{a}]$ and the Reason $[\mathrm{r}]$.Assertion $[\mathrm{a}]$: In multicellular organisms, cells o...

admin

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admin asked May 21, 2023

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47

GATE BT 2022 | Question: 51
Consider a piston-cylinder assembly shown in the figure below. The walls of the cylinder are insulated. The cylinder contains $1$ mole of an ideal gas at $300 \; \text{K}$ and the piston is held initially at the position $\text{z}_{1}$ using a stopper. ... $\text{K}.$

Consider a piston-cylinder assembly shown in the figure below. The walls of the cylinder are insulated. The cylinder contains $1$ mole of an ideal gas at $300 \; \text{K}...

Lakshman Bhaiya

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Lakshman Bhaiya asked Mar 20, 2022

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48

GATE BT 2021 | Question: 26
Which one of the following represents non-growth associated product formation kinetics in a bioprocess system? $X$ and $P$ denote viable cell and product concentrations, respectively.

Which one of the following represents non-growth associated product formation kinetics in a bioprocess system? $X$ and $P$ denote viable cell and product concentrations, ...

go_editor

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go_editor asked Mar 1, 2021

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49

GATE2019: 55
The dimensions and operating condition of a lab-scale fermentor are as follows: Volume = $1\:L$ Diameter = $20$ cm Agitator speed = $600$ rpm Ratio of impeller diameter to fermentor diameter = $0.3$ ... the agitator in the larger reactor is ___________ rpm. Assume that the scale-up factor is the cube root of the ratio of fermentor volumes.

The dimensions and operating condition of a lab-scale fermentor are as follows:Volume = $1\:L$Diameter = $20$ cmAgitator speed = $600$ rpmRatio of impeller diameter to fe...

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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50

GATE2014-33
Triose phosphate isomerase converts dihydroxy acetone phosphate $(DHAP)$ to glyceraldehyde-3-phosphate $(G-3-P)$ in a reversible reaction. At $\text{298 K and pH 7.0,}$ the equilibrium mixture contains $\text{40 mM DHAP and 4 mM G-3-P.}$ ... -energy change in $\text{kJ/mol}$ for the formation of $\text{G-3-P [R = 8.315 J/mol.K]}$ is __________.

Triose phosphate isomerase converts dihydroxy acetone phosphate $(DHAP)$ to glyceraldehyde-3-phosphate $(G-3-P)$ in a reversible reaction. At $\text{298 K and pH 7.0,}$ t...

Milicevic3306

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Milicevic3306 asked Mar 25, 2018

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51

GATE BT 2021 | Question: 42
In a chemostat with a dilution rate of $0.8$ $h^{-1}$, the steady state biomass concentration and the specific product formation rate are $8 \textit{ mol m}^{-3}$ and $0.2 \;(\textit{mol product}) (\textit{mol biomass})^{-1} \: h^{-1}$, respectively. The steady state product concentration in $\textit{mol m}^{-3}$ is ___________

In a chemostat with a dilution rate of $0.8$ $h^{-1}$, the steady state biomass concentration and the specific product formation rate are $8 \textit{ mol m}^{-3}$ and $0....

go_editor

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go_editor asked Mar 1, 2021

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52

GATE BT 2023 | Question: 20
Fabry disease in humans is a X-linked disease. The probability (in percentage) for a phenotypically normal father and a carrier mother to have a son with Fabry disease is _________.

Fabry disease in humans is a X-linked disease. The probability (in percentage) for a phenotypically normal father and a carrier mother to have a son with Fabry disease is...

admin

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admin asked May 21, 2023

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53

GATE2018-7
Which one of the following BLAST search programs is used to identify homologs of a genomic DNA query in a protein sequence database? blastp blastn blastx tblastn

Which one of the following BLAST search programs is used to identify homologs of a genomic DNA query in a protein sequence database?blastpblastnblastxtblastn

gatecse

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gatecse asked Feb 20, 2018

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54

GATE2019: 46
The difference in concentrations of an uncharged solute between two compartments is $1.6$-fold. The energy required for active transport of the solute across the membrane separating the two compartments is _________ $cal\:mol^{-1}$ (rounded off to the nearest integer). $(R =1.987\:cal\:mol^{-1}\:K^{-1},\:T=37 \;^{\circ}C)$

The difference in concentrations of an uncharged solute between two compartments is $1.6$-fold. The energy required for active transport of the solute across the membrane...

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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55

GATE BT 2023 | Question: 47
A protein has three identical sites arranged at the vertices of an equilateral triangle. If one site is filled with a dye (donor), the measured quantum yield $\left(\phi_{D}\right)$ is $0.5$. Filling one site with a donor dye and a ... of one site filled with donor and the other two sites filled with acceptor dye (rounded off to three decimal places) is _____________.

A protein has three identical sites arranged at the vertices of an equilateral triangle. If one site is filled with a dye (donor), the measured quantum yield $\left(\phi_...

admin

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admin asked May 21, 2023

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56

GATE2018-49
Yeast converts glucose to ethanol and carbon dioxide by glycolysis as per the following reaction: $C_6H_{12}O_6 \to 2C_2H_5OH + 2CO_2$Assuming complete conversion, the amount of ethanol produced (in $g$) from $200 \: g$ of glucose is (up to two decimal places) ____________

Yeast converts glucose to ethanol and carbon dioxide by glycolysis as per the following reaction: $$C_6H_{12}O_6 \to 2C_2H_5OH + 2CO_2$$Assuming complete conversion, the ...

gatecse

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gatecse asked Feb 20, 2018

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57

GATE BT 2021 | Question: 21
Three balls, colored in blue, green and red, are successively transferred from box $A$ to box $B$ in the order $\text{BLUE-GREEN-RED.}$ The probability of a reverse transfer of the balls to the box $A$ in the same order (rounded off to two decimal places) is ____________

Three balls, colored in blue, green and red, are successively transferred from box $A$ to box $B$ in the order $\text{BLUE-GREEN-RED.}$ The probability of a reverse trans...

go_editor

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go_editor asked Mar 1, 2021

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58

GATE2012-54
$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and a voidage ($\epsilon$) of $0.55$. The $K'_m$ and $V'_m$; for the immobilized enzyme are $0.72 \: gl^{-1}$ and ... time required for the steady state reactor operation will be $0.1 \: h$ $0.4 \: h$ $1.0 \: h$ $1.1 \: h$

$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and...

Milicevic3306

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Milicevic3306 asked Mar 25, 2018

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59

GATE2018-1
Consider an unfair coin. The probability of getting heads is $0.6$. If you toss this coin twice, what is the probability that the fast or the second toss is heads? $0.56$ $0.64$ $0.84$ $0.96$

Consider an unfair coin. The probability of getting heads is $0.6$. If you toss this coin twice, what is the probability that the fast or the second toss is heads?$0.56$$...

gatecse

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gatecse asked Feb 20, 2018

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60

GATE2018-23
The absorbance of a solution of tryptophan measured at $280$ nm in a cuvette of $2.0$ cm path length is $0.56$ at pH $7$. The molar extinction coefficient $(\varepsilon)$ for tryptophan at $280$ nm is $5600$ $M^{-1}$ $cm^{-1}$ at pH $7$. The concentration of tryptophan (in $\mu M$) in the solution is __________

The absorbance of a solution of tryptophan measured at $280$ nm in a cuvette of $2.0$ cm path length is $0.56$ at pH $7$. The molar extinction coefficient $(\varepsilon)$...

gatecse

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gatecse asked Feb 20, 2018

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1 answer

61

GATE BT 2023 | GA Question: 4
In $2022$, June Huh was awarded the Fields medal, which is the highest prize in Mathematics. When he was younger, he was also a poet. He did not win any medals in the International Mathematics Olympiads. He dropped out of college. Based ... of college has won the Fields medal. All Fields medalists are part-time poets. Some Fields medalists have dropped out of college.

In $2022$, June Huh was awarded the Fields medal, which is the highest prize in Mathematics.When he was younger, he was also a poet. He did not win any medals in the Inte...

admin

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admin asked May 21, 2023

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1 answer

62

GATE BT 2023 | Question: 36
The following reaction represents biomass synthesis from hexadecane ... represents the biomass. The value of respiratory quotient (rounded off to two decimal places) is ___________.

The following reaction represents biomass synthesis from hexadecane$\mathrm{C}_{16} \mathrm{H}_{34}+12.5 \mathrm{O}_2+2.13 \mathrm{NH}_3 \rightarrow 10.6 \mathrm{CH}_{1.6...

admin

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admin asked May 21, 2023

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63

GATE2016-13
Which one of the following techniques relies on the spin angular momentum of a photon? CD spectroscopy Fluorescence spectroscopy IR spectroscopy Raman spectroscopy

Which one of the following techniques relies on the spin angular momentum of a photon?CD spectroscopyFluorescence spectroscopyIR spectroscopyRaman spectroscopy

Milicevic3306

7.9k points

Milicevic3306 asked Mar 26, 2018

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64

GATE BT 2022 | Question: 18
A microorganism is grown in a batch culture using glucose as a carbon source. The apparent growth yield is $0.5 \frac{\text{g biomass}}{\text{g substrate}}.$ ... that there is no endogenous metabolism, the maximum biomass concentration that can be achieved is _________ $ \text{g L}^{-1}.$

A microorganism is grown in a batch culture using glucose as a carbon source. The apparent growth yield is $0.5 \frac{\text{g biomass}}{\text{g substrate}}.$ The initial ...

Lakshman Bhaiya

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Lakshman Bhaiya asked Mar 20, 2022

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65

GATE2013-22
A chemostat is operated at a dilution rate of $0.6\; h^{-1}$. At steady state, the biomass concentration in the exit stream was found to be $30\; gl^{-1}$The biomass productivity $(g\; l^{-1}h^{-1})$ after $3h$ of steady state operation will be ___________.

A chemostat is operated at a dilution rate of $0.6\; h^{-1}$. At steady state, the biomass concentration in the exit stream was found to be $30\; gl^{-1}$The biomass prod...

Milicevic3306

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Milicevic3306 asked Mar 25, 2018

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66

GATE2019: 17
Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation? internal reserves extracellular substrates extracellular products toxic substrates

Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation?internal reservesextracellular substratesextracellular productstox...

soujanyareddy13

2.7k points

soujanyareddy13 asked Nov 3, 2020

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67

GATE BT 2023 | Question: 52
The values of the consistency index ' $K$ ' and the flow behavior index ' $n$ ' of a dilatant fluid are 0.415 (in CGS units) and $1.23$, respectively. The value of the apparent viscosity (in $g . \mathrm{cm}^{-1} . \mathrm{s}^{-1}$ ) of this fluid at a shear rate of $60 \mathrm{~s}^{-1}$ (rounded off to the nearest integer) is _____________.

The values of the consistency index ' $K$ ' and the flow behavior index ' $n$ ' of a dilatant fluid are 0.415 (in CGS units) and $1.23$, respectively. The value of the ap...

admin

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admin asked May 21, 2023

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68

GATE2018-13
Which one of the following bioreactor configurations is the basis for a trickling biological filter? Stirred tank Packed bed Air lift Fluidized bed

Which one of the following bioreactor configurations is the basis for a trickling biological filter?Stirred tankPacked bedAir liftFluidized bed

gatecse

1.4k points

gatecse asked Feb 20, 2018

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69

GATE2020: 52
Protein $A$ and protein $B$ form a covalent complex. Gel filtration chromatography of this complex showed a peak corresponding to $200 \:kDa$. $SDS-PAGE$ analysis of this complex, with and without beta-mercaptoethanol, showed a single band corresponding to molecular ... the molecular weight of protein $A$ is $25 \:kDa$, the molecular weight of protein $B$ is ________________ $kDa$.

Protein $A$ and protein $B$ form a covalent complex. Gel filtration chromatography of this complex showed a peak corresponding to $200 \:kDa$. $SDS-PAGE$ analysis of this...

soujanyareddy13

2.7k points

soujanyareddy13 asked Nov 3, 2020

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70

GATE2013-23
A batch bioreactor is to be scaled up from $\text{10 to 10,000}$ liters. The diameter of the large bioreactor is $10$ times that of the small bioreactor. The agitator speed in the small bioreactor is $\text{450 rpm}$. Determine the agitator speed (rpm) of the large bioreactor with same impeller tip speed as that of the small bioreactor. ____________

A batch bioreactor is to be scaled up from $\text{10 to 10,000}$ liters. The diameter of the large bioreactor is $10$ times that of the small bioreactor. The agitator spe...

Milicevic3306

7.9k points

Milicevic3306 asked Mar 25, 2018

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71

GATE2018-2
If serum is removed from the growth medium of human embryonic kidney cell line (HEK), then the cells will proliferate faster proliferate normally undergo cell cycle arrest undergo immediate apoptosis

If serum is removed from the growth medium of human embryonic kidney cell line (HEK), then the cells willproliferate fasterproliferate normallyundergo cell cycle arrestun...

gatecse

1.4k points

gatecse asked Feb 20, 2018

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72

GATE2017-54
During anaerobic growth, an organism converts glucose $(P)$ into biomass $(Q)$, ethanol $(R)$, acetaldehyde $(S)$, and glycerol $(T)$ ... , the ethanol produced (in grams) is ________ Given data: Atomic weights (Da) of $C=12, H=1, O=16$, and $N=14$

During anaerobic growth, an organism converts glucose $(P)$ into biomass $(Q)$, ethanol $(R)$, acetaldehyde $(S)$, and glycerol $(T)$. Every mole of carbon present in glu...

Milicevic3306

7.9k points

Milicevic3306 asked Mar 26, 2018

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73

GATE2020: 22
To facilitate mass transfer from a gas to a liquid phase, a gas bubble of radius $r$ is introduced into the liquid. The gas bubble then breaks into $8$ bubbles of equal radius. Upon this change, the ratio of the interfacial surface area to the gas phase volume for the system changes from $3/r$ to $3n/r$. The value of $n$ is _____________________.

To facilitate mass transfer from a gas to a liquid phase, a gas bubble of radius $r$ is introduced into the liquid. The gas bubble then breaks into $8$ bubbles of equal r...

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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74

GATE2016-36
A $1.2$ kb DNA fragment was cloned into $\textit{Bam}$HI and $\textit{Eco}$RI sites located on a $2.8$ kb cloning vector. The $\textit{Bam}$HI and $\textit{Eco}$RI sites are adjacent to each other on the vector backbone. The vector contains an $\textit{XhoI}$ site located $300$ ... to: $2800$, $700$ and $500$ $2800$, $700$ and $800$ $2500$, $700$ and $800$ $2500$, $1200$ and $300$

A $1.2$ kb DNA fragment was cloned into $\textit{Bam}$HI and $\textit{Eco}$RI sites located on a $2.8$ kb cloning vector. The $\textit{Bam}$HI and $\textit{Eco}$RI sites ...

Milicevic3306

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Milicevic3306 asked Mar 26, 2018

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75

GATE2020: 21
The number of molecules of a nucleotide of molecular weight $300\:g$/mol present in $10$ picomoles is ____________ $\times10^{12}$ (round off to $2$ decimal places).

The number of molecules of a nucleotide of molecular weight $300\:g$/mol present in $10$ picomoles is ____________ $\times10^{12}$ (round off to $2$ decimal places).

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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1 answer

76

GATE2018-29
Determine the correctness or otherwise of the following Assertion [a] and the Reason [r] Assertion: Ab initio gene finding algorithms that predict protein coding genes in eukaryotic genomes are not completely accurate. Reason: Eukaryotic splice sites are difficult to predict. Both [a] and [ ... correct reason for [a] Both [a] and [r] are true but [r] is not the correct reason for [a]

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r]Assertion: Ab initio gene finding algorithms that predict protein coding genes in ...

gatecse

1.4k points

gatecse asked Feb 20, 2018

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77

GATE2021
A batch cultivation of E. coli follows zeroth order Monod's growth kinetics. The cell growth is terminated when the residual dissolved oxygen concentration attains 10% of its saturation value and oxygen mass transfer coefficient ( ) L k a reaches its maximum value ( ) 1 ... the final cell concentration in 3 kg m− (rounded off to two decimal places) at the end of the batch cultivation is _

A batch cultivation of E. coli follows zeroth order Monod’s growth kinetics. The cell growth is terminated when the residual dissolved oxygen concentration attains 10% ...

DARSHAN KJ

120 points

DARSHAN KJ asked Oct 20, 2023

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78

GATE2020: 45
A batch reactor is inoculated with $1 \:g/L$ biomass. Under these conditions, cells exhibit a lag phase of $30$ min. If the specific growth rate in the log phase is $0.00417\;min^{-1}$, the time taken for the biomass to increase to $8 \:g/L$ is ________________ min (round off to $2$ decimal places).

A batch reactor is inoculated with $1 \:g/L$ biomass. Under these conditions, cells exhibit a lag phase of $30$ min. If the specific growth rate in the log phase is $0.00...

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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79

GATE BT 2021 | Question: 43
If the values of two random variables $(X,Y)$ are $(121, 360)$, $(242, 364)$ and $(363, 362)$, the value of correlation coefficient between $X$ and $Y$ (rounded off to one decimal place) is ________.

If the values of two random variables $(X,Y)$ are $(121, 360)$, $(242, 364)$ and $(363, 362)$, the value of correlation coefficient between $X$ and $Y$ (rounded off to on...

go_editor

1.4k points

go_editor asked Mar 1, 2021

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80

GATE2019: 43
Which of the following statements is $ALWAYS\; CORRECT$ about an ideal chemostat? Substrate concentration inside the chemostat is equal to that in the exit stream Optimal dilution rate is lower than critical dilution rate Biomass concentration increases with increase in dilution rate Cell recirculation facilitates ... $Q$ only $P, R$ and $S$ only $P$ and $S$ only $P, Q$ and $S$ only

Which of the following statements is $ALWAYS\; CORRECT$ about an ideal chemostat?Substrate concentration inside the chemostat is equal to that in the exit streamOptimal d...

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020