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Common Data for Questions 50 and 51:The purication data for an enzyme is given below:Step Total protein Total activity Specific activity- nits Units-m)Cellfree extract 177 102 0.58O- Seharose 18.8 72 3.83Phenvl Seoharose 9.2 45 4.89ehacrvl 8-200 4.1 30 7.32 Q.50 The fold purification for each step is

  1. P-0.1= Q- 0.66. R0.84, S- 1.26
  2. P-1.0, Q-O.52= RO.67, 5-0.8
  3. Pl. Q-6.6= R-8.4, Sl2-6
  4. P-lOO= Q-66, R-84. S-12
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