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Answer the question based on the information given below:

The purification data for an enzyme is given below:

$\begin{array}{|l|l|l|l|l|l|} \hline & \text{Step} & \text{Volume} & \text{Total protein} & \text{Total activity} & \text{Specific activity} \\ && \text{(ml)} & \text{(mg)} & \text{Units} & \text{Units/mg}  \\ \hline P & \text{Cell-free extract} & 17 & 177 & 102 & 0.58 \\ \hline Q & \text{Q-Sepharose} & 14 & 18.8  & 72 & 3.83 \\ \hline R & \text{Phenyl Sepharose} &26 & 9.2 & 45 & 4.89 \\ \hline S & \text{Sephacryl S}-200 & 7 & 4.1 & 30 & 7.32 \\ \hline \end{array}$

The fold purification for each step is

  1. $P-0.1, Q- 0.66, R-0.84, S- 1.26$
  2. $P-1.0, Q- 0.52, R-0.67, S- 0.8$
  3. $P-1, Q- 6.6, R-8.4, S- 12.6$
  4. $P-100, Q- 66 , R-84, S- 12$
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