GATE2012-53

$\text{Statement for Linked Answer Questions 52 and 53:}$

An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding affinity (${K_d}$) of $10^{-8}$ M and $10^{-9}$ M with and without lactose respectively, in the medium

Therefore the lac-operon is

1. repressed and can only be induced with lactose
2. repressed and cannot be induced with lactose
3. not repressed
4. expressed only when glucose and lactose are present
in Others
edited

Related questions

1
$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and a voidage ($\epsilon$) of $0.55$. The $K'_m$ and $V'_m$; for the immobilized enzyme are $0.72 \: gl^{-1}$ ... for the above bioconversion will be $50 \: lh^{-1}$ $55 \: lh^{-1}$ $137 \: lh^{-1}$ $550 \: lh^{-1}$
2
$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and a voidage ($\epsilon$) of $0.55$. The $K'_m$ and $V'_m$; for the immobilized enzyme are $0.72 \: gl^{-1}$ ... time required for the steady state reactor operation will be $0.1 \: h$ $0.4 \: h$ $1.0 \: h$ $1.1 \: h$
$\text{Statement for Linked Answer Questions 52 and 53:}$ An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding affinity (${K_d}$) of $10^{-8}$ M and $10^{-9}$ M with and without lactose respectively, in the medium The molar concentration of the repressor in the cell 15 $\text{0.1 nM}$ $\text{1 nM}$ $\text{10 nM}$ $\text{100 nM}$
Answer the question based on the information given below: The purification data for an enzyme is given below: ... $\text{P-3.4, Q-2.4, R-1.5, S-0.1}$ $\text{P-100, Q-71, R-44, S-29}$
Answer the question based on the information given below: The purification data for an enzyme is given below: ... $P-1, Q- 6.6, R-8.4, S- 12.6$ $P-100, Q- 66 , R-84, S- 12$