GATE2013-11

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018 edited Nov 4, 2020 by soujanyareddy13

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Phylum proteobacteria is subdivided into $\alpha-, \beta-, \gamma-, \delta-$ and $\epsilon-$ proteobacteria based on

$G+C$ content
$23S\; rRNA$ sequences
$tRNA$ sequences
$16S\; rRNA$ sequences

gate2013
dna-rna

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018 edited Nov 4, 2020 by soujanyareddy13

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Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

GATE2013-42
A complete restriction digestion of a circular plasmid $(5000bp)$ was carried out with $HindIII,BamHIandEcoRIindividually.$ Restriction digestion yielded following fragments. $Plasmid + HindIII \rightarrow 1200bp\: and\: 3800bp$ $Plasmid + BamHI \rightarrow 5000bp$ ... $EcoRl-3, BamHI-2, HindIII-1$ $EcoRI-2, BamHI-2, HindIII-1$

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The suitable substitution matrix to align closely related sequences is $\text{PAM 250 or BLOSSUM 80}$ $\text{PAM 40 or BLOSSUM 80}$ $\text{PAM 120 or BLOSSUM 40}$ $\text{PAM 250 or BLOSSUM 40}$

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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