# GATE2013-11

Phylum proteobacteria is subdivided into $\alpha-, \beta-, \gamma-, \delta-$ and $\epsilon-$ proteobacteria based on

1. $G+C$ content
2. $23S\; rRNA$ sequences
3. $tRNA$ sequences
4. $16S\; rRNA$ sequences

edited

## Related questions

1
Match the entries in $\text{Group I}$ with the entries in $\text{Group II}$ ... $\text{P-4, Q-3, R-2, S-1}$ $\text{P-3, Q-2, R-1, S-4}$ $\text{P-2, Q-4, R-1, S-3}$
2
Under alkaline conditions, DNA is more stable than RNA because RNA forms secondary structures RNA is a single stranded molecule RNA has uracil in place of thymidine RNA is susceptible to hydrolysis
A DNA fragment of 5000bp needs to be isolated from $E.coli$ (genome size $4\times10^3 kb$) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are $16\times10^2$ $12\times10^2$ $8\times10^2$ $1.25\times10^2$
A complete restriction digestion of a circular plasmid $(5000bp)$ was carried out with $HindIII,BamHIandEcoRIindividually.$ Restriction digestion yielded following fragments. $Plasmid + HindIII \rightarrow 1200bp\: and\: 3800bp$ $Plasmid + BamHI \rightarrow 5000bp$ $Plasmid + EcoRI \rightarrow 2500bp$ ... $EcoRI-1, BamHI- 1, HindIII-2$ $EcoRl-3, BamHI-2, HindIII-1$ $EcoRI-2, BamHI-2, HindIII-1$
The suitable substitution matrix to align closely related sequences is $\text{PAM 250 or BLOSSUM 80}$ $\text{PAM 40 or BLOSSUM 80}$ $\text{PAM 120 or BLOSSUM 40}$ $\text{PAM 250 or BLOSSUM 40}$