0 votes
0 votes

A complete restriction digestion of a circular plasmid $(5000bp)$ was carried out with $HindIII,BamHIandEcoRIindividually.$ Restriction digestion yielded following fragments. 

$Plasmid + HindIII \rightarrow 1200bp\: and\: 3800bp$

$Plasmid + BamHI \rightarrow 5000bp$

$Plasmid + EcoRI \rightarrow 2500bp$

The number of sites for $EcoRI,BamHI\:and\:HindIII $ present on this plasmid are

  1. $EcoRl-2, BamHI- 1, HindIII-2$
  2. $EcoRI-1, BamHI- 1, HindIII-2$
  3. $EcoRl-3, BamHI-2, HindIII-1$
  4. $EcoRI-2, BamHI-2, HindIII-1$
in Recombinant DNA technology and Other Tools in Biotechnology edited by
7.9k points

Please log in or register to answer this question.


Related questions

Welcome to GATE BioTechnology, where you can ask questions and receive answers from other members of the community.