Log In
Welcome to GATE BioTechnology, where you can ask questions and receive answers from other members of the community.
0 votes

A complete restriction digestion of a circular plasmid $(5000bp)$ was carried out with $HindIII,BamHIandEcoRIindividually.$ Restriction digestion yielded following fragments. 

$Plasmid + HindIII \rightarrow 1200bp\: and\: 3800bp$

$Plasmid + BamHI \rightarrow 5000bp$

$Plasmid + EcoRI \rightarrow 2500bp$

The number of sites for $EcoRI,BamHI\:and\:HindIII $ present on this plasmid are

  1. $EcoRl-2, BamHI- 1, HindIII-2$
  2. $EcoRI-1, BamHI- 1, HindIII-2$
  3. $EcoRl-3, BamHI-2, HindIII-1$
  4. $EcoRI-2, BamHI-2, HindIII-1$
in Recombinant DNA Technology 7.9k points
edited by

Please log in or register to answer this question.