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A DNA fragment of 5000bp needs to be isolated from $E.coli$ (genome size $4\times10^3 kb$) genomic library.

The minimum number of independent recombinant clones required to represent this fragment in genomic library are

  1. $16\times10^2$
  2. $12\times10^2$
  3. $8\times10^2$
  4. $1.25\times10^2$
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