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The intemction between an antigen (Ag) and a single-chain antibody (Ab) was studied usingScatchard analysis. The result is shown below.Y[Ab-A31][Ag]0 X[Ab-A8] The affinity of interaction and the total concentration of antibody. respectively. can be determinedfrom

  1. slope and Y-intercept
  2. Y-intercept and slope
  3. X-intercept and slope
  4. slope and X-intercept
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