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The interaction between an antigen $(Ag)$ and a single-chain antibody $(Ab)$ was studied using Scatchard analysis. The result is shown below.

The affinity of interaction and the total concentration of antibody, respectively, can be determined from

  1. slope and $Y$-intercept
  2. $Y$-intercept and slope
  3. $X$-intercept and slope
  4. slope and $X$-intercept
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