Bio Technology
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A recomhimnt protein is to be exprexxed under the control of the fur promoter and operator in a
xuain ot‘E m/i having the genotwe Incl” rrp’ Even in the absence of inducer IPTG. lo“ levels of
expression ofthe recombinant protein are seen (leaky exprexsion i. Which one of the following
should he done to minimize such leaky expression?

(A) Addition of lactose to the medium
(B) Remox'al of all glucose from the medium

(C) Addition of excexs glucose to the medium
(D) Addition of (Ilia-lactose to the medium

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