# GATE2017-35

The specific activity of an enzyme in a crude extract of $\textit{E.coli}$ is $9.5$ units/mg of protein. The specific activity increased to $68$ units/mg of protein upon ion-exchange chromatography. The fold purification is ________
in Others
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## Related questions

1
In a thin layer chromatography experiment using a silica gel plate, a compound showed migration of $12.5$ cm and the solvent front showed migration of $18$ cm. The $R_f$ value for the compound is ______
2
$\underset{x \to 0}{\lim} \dfrac{\sin(x)}{x}$ is ________
A $5$ liter chemostat is fed fresh medium at $0.2$ liters/minute having a substrate concentration of $25$ grams/liter. At steady state, the outgoing stream has substrate concentration of $2.5$ grams/liter. The rate of consumption (grams/liter/minute) of the substrate in the reactor is __________
At the transcription start site of a gene, any of the four nucleotides can occur with equal probability $p$. The Shannon Entropy $S$, given by $S= – \Sigma_{i=1}^4 p_i \ln p_i$, for this start site is _________