GATE2012-7

Milicevic3306 asked in Others Mar 25, 2018 edited Oct 24, 2020 by soujanyareddy13

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Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because

it is an organic solvent
it easily penetrates cells
it protects cells by preventing crystallization of water
it is also utilized as a nutrient

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Milicevic3306 asked in Others Mar 25, 2018 edited Oct 24, 2020 by soujanyareddy13

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Milicevic3306 asked in Others Mar 25, 2018

GATE2012-55
$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and a voidage ($\epsilon$) of $0.55$. The $K'_m$ and $V'_m$; for the immobilized enzyme are $0.72 \: gl^{-1}$ ... the above bioconversion will be $50 \: lh^{-1}$ $55 \: lh^{-1}$ $137 \: lh^{-1}$ $550 \: lh^{-1}$

Milicevic3306 asked in Others Mar 25, 2018

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Milicevic3306 asked in Others Mar 25, 2018

GATE2012-54
$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and a voidage ($\epsilon$) of $0.55$. The $K'_m$ and $V'_m$; for the immobilized enzyme are $0.72 \: gl^{-1}$ and ... time required for the steady state reactor operation will be $0.1 \: h$ $0.4 \: h$ $1.0 \: h$ $1.1 \: h$

Milicevic3306 asked in Others Mar 25, 2018

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Milicevic3306 asked in Others Mar 25, 2018

GATE2012-53
$\text{Statement for Linked Answer Questions 52 and 53:}$ An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding affinity (${K_d}$) of $10^{-8}$ ... operon is repressed and can only be induced with lactose repressed and cannot be induced with lactose not repressed expressed only when glucose and lactose are present

Milicevic3306 asked in Others Mar 25, 2018

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gate2012

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Milicevic3306 asked in Others Mar 25, 2018

GATE2012-52
$\text{Statement for Linked Answer Questions 52 and 53:}$ An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding affinity (${K_d}$) of $10^{-8}$ M and $10^{-9}$ M with and without lactose respectively, in the ... The molar concentration of the repressor in the cell 15 $\text{0.1 nM}$ $\text{1 nM}$ $\text{10 nM}$ $\text{100 nM}$

Milicevic3306 asked in Others Mar 25, 2018

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gate2012

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Milicevic3306 asked in Others Mar 25, 2018

GATE2012-51
Answer the question based on the information given below: The purification data for an enzyme is given below: ... $\text{P-3.4, Q-2.4, R-1.5, S-0.1}$ $\text{P-100, Q-71, R-44, S-29}$

Milicevic3306 asked in Others Mar 25, 2018

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