GATE Overflow for GATE BT - Recent questions and answers in Upstream and Downstream Processing

Answered: GATE2016-17

Sat, 12 Sep 2026 20:24:52 +0000

option C

GATE BT 2025 | Question: 17

Thu, 06 Mar 2025 17:05:43 +0000

A recombinant protein is secreted extracellularly in soluble form by an $E. coli$ culture. Which of the following downstream processes is/are involved in the purification of the extracellular secreted protein?

Cell disruption
Membrane ultrafiltration
Solubilisation of inclusion bodies
Liquid chromatography

GATE BT 2023 | Question: 39

Sun, 21 May 2023 02:58:22 +0000

Aqueous two-phase extraction is used to recover } \alpha \text {-amylase from a solution. A polypropylene glycol-dextran mixture is added and the solution separates into upper and lower phases. The partition coefficient is $4.0$ and the ratio of upper to lower phase volume is $5.0$. The enzyme recovery or yield (in percentage, rounded off to the nearest integer) is ______________.

GATE BT 2022 | Question: 4

Sun, 20 Mar 2022 16:16:30 +0000

Match the media component (Column I) with its role (Column II).

$$\begin{array} {ll} \textbf{Column I} & \textbf{Column I} \\ \text{P. Sucrose} & \text{1. Anti-foam agent} \\ \text{Q. Zinc chloride} & \text{2. Nitrogen source} \\ \text{R. Ammonium sulphate} & \text{3. Carbon source} \\ \text{S. Silicone oil} & \text{4. Trace element} \end{array}$$

$\text{P-1, Q-2, R-3, S-4}$
$\text{P-2, Q-1, R-3, S-4}$
$\text{P-3, Q-2, R-4, S-1}$
$\text{P-3, Q-4, R-2, S-1}$

GATE BT 2021 | Question: 28

Mon, 01 Mar 2021 03:19:48 +0000

Match separation methods in $\text{Group I}$ with associated properties in $\text{Group II}$.

$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \textbf{Group II} \\ \hline P. & \text{Centrifugation} & 1. & \text{Density} \\ \hline Q. & \text{Dialysis} & 2. & \text{Diffusivity} \\ \hline R. & \text{Solvent extraction} & 3. & \text{Size} \\ \hline S. & \text{Ultrafilration} & 4. & \text{Solubility} \\ \hline \end{array}$$

$P-4, Q-2, R-1, S-3$
$P-3, Q-1, R-2, S-4$
$P-1, Q-3, R-2, S-4$
$P-1, Q-2, R-4, S-3$

GATE2016-21

Mon, 26 Mar 2018 16:59:50 +0000

Which one of the following is the most suitable type of impeller for mixing high viscosity (viscosity $> 10^5$ cP) fluids?

Propeller
Helical ribbon
Paddle
Flat blade turbine

GATE BT 2012 | Question: 38

Sun, 25 Mar 2018 08:38:46 +0000

Match the downstream processes in $\text{Group I}$ with the products in $\text{Group II}$.

$\begin{array}{|C|l|C|l|} \hline& \textbf{Group I} & {} & \textbf{Group II} \\\hline P. &\text{Solvent extraction} & 1. &\text{Lactic acid} \\\hline Q. &\text{Protein-A linked affinity chromatography} & 2. &\text{Penicillin} \\\hline R. &\text{Extractive distillation} & 3. &\text{Monoclonal antibody} \\\hline S. &\text{Salting out} & 4. &\text{Lipase} \\\hline\end{array}$

$\text{P-2, Q-3, R-1, S-4}$
$\text{P-4, Q-1, R-2, S-3}$
$\text{P-4, Q-1, R-3, S-2}$
$\text{P-2, Q-4, R-1, S-3}$