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<title>GATE Overflow for GATE BT - Questions without answers in General Biology</title>
<link>https://bt.gateoverflow.in/unanswered/general-biology</link>
<description>Powered by Question2Answer</description>
<item>
<title>GATE BT 2026 | Question: 4</title>
<link>https://bt.gateoverflow.in/1317/gate-bt-2026-question-4</link>
<description>&lt;p&gt;Cancer cells display a high level of heterogeneity in the expression of TP$53$ protein. Which of the following techniques can be directly used to determine the intratumoral heterogeneity of TP$53$ expression in a given tumor sample, without resorting to additional cell separation techniques?&lt;/p&gt;&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;&lt;li&gt;Immunoblotting&lt;/li&gt;&lt;li&gt;Flow cytometry&lt;/li&gt;&lt;li&gt;ELISA&lt;/li&gt;&lt;li&gt;Quantitative RT-PCR&lt;/li&gt;&lt;/ol&gt;</description>
<category>Analytical Techniques</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1317/gate-bt-2026-question-4</guid>
<pubDate>Mon, 23 Feb 2026 14:22:26 +0000</pubDate>
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<title>GATE BT 2026 | Question: 7</title>
<link>https://bt.gateoverflow.in/1314/gate-bt-2026-question-7</link>
<description>&lt;p&gt;In nitrogen fixation, to reduce nitrogen to ammonia, which one of the following shows the correct order of electron flow?&lt;/p&gt;&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;&lt;li&gt;Ferredoxin → reductase → nitrogenase&lt;/li&gt;&lt;li&gt;NADH → reductase → nitrogenase&lt;/li&gt;&lt;li&gt;Ferredoxin → nitrogenase → reductase&lt;/li&gt;&lt;li&gt;Reductase → NADH → nitrogenase&lt;/li&gt;&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1314/gate-bt-2026-question-7</guid>
<pubDate>Mon, 23 Feb 2026 14:22:17 +0000</pubDate>
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<title>GATE BT 2026 | Question: 8</title>
<link>https://bt.gateoverflow.in/1313/gate-bt-2026-question-8</link>
<description>&lt;p&gt;For monoclonal antibody production, hypoxanthine $\_\_\_\_$.&lt;/p&gt;&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;&lt;li&gt;allows DNA synthesis in the absence of de novo dTMP synthesis&lt;/li&gt;&lt;li&gt;allows purine synthesis in cells containing HGPRT&lt;/li&gt;&lt;li&gt;induces cell fusion&lt;/li&gt;&lt;li&gt;prevents de novo nucleotide synthesis&lt;/li&gt;&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1313/gate-bt-2026-question-8</guid>
<pubDate>Mon, 23 Feb 2026 14:22:13 +0000</pubDate>
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<title>GATE BT 2026 | Question: 9</title>
<link>https://bt.gateoverflow.in/1312/gate-bt-2026-question-9</link>
<description>&lt;p&gt;Which one of the following proteins would elute third in gel filtration chromatography from a mixture of four proteins given below?&lt;/p&gt;&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;&lt;li&gt;Insulin $(6&amp;nbsp;\text{ kDa})$&lt;/li&gt;&lt;li&gt;Myoglobin&amp;nbsp;$(17&amp;nbsp;\text{ kDa})$&lt;/li&gt;&lt;li&gt;Thrombin&amp;nbsp;$(37&amp;nbsp;\text{ kDa})$&lt;/li&gt;&lt;li&gt;Albumin&amp;nbsp;$(66&amp;nbsp;\text{ kDa})$&lt;/li&gt;&lt;/ol&gt;</description>
<category>Analytical Techniques</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1312/gate-bt-2026-question-9</guid>
<pubDate>Mon, 23 Feb 2026 14:22:11 +0000</pubDate>
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<item>
<title>GATE BT 2026 | Question: 10</title>
<link>https://bt.gateoverflow.in/1311/gate-bt-2026-question-10</link>
<description>&lt;p&gt;Which one of the following antibiotics blocks protein chain elongation by preventing the action of peptidyl transferase?&lt;/p&gt;&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;&lt;li&gt;Bleomycin&lt;/li&gt;&lt;li&gt;Rifampicin&lt;/li&gt;&lt;li&gt;Chloramphenicol&lt;/li&gt;&lt;li&gt;Tetracycline&lt;/li&gt;&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1311/gate-bt-2026-question-10</guid>
<pubDate>Mon, 23 Feb 2026 14:22:08 +0000</pubDate>
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<title>GATE BT 2026 | Question: 12</title>
<link>https://bt.gateoverflow.in/1309/gate-bt-2026-question-12</link>
<description>&lt;p&gt;The RNA primer synthesized during bacterial DNA replication is removed by $\_\_\_\_$.&lt;/p&gt;&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;&lt;li&gt;DNA gyrase&lt;/li&gt;&lt;li&gt;DNA Polymerase $\text{I}$&lt;/li&gt;&lt;li&gt;Primase&lt;/li&gt;&lt;li&gt;DNA Polymerase $\text{III}$&lt;/li&gt;&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1309/gate-bt-2026-question-12</guid>
<pubDate>Mon, 23 Feb 2026 14:22:02 +0000</pubDate>
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<title>GATE BT 2026 | Question: 13</title>
<link>https://bt.gateoverflow.in/1308/gate-bt-2026-question-13</link>
<description>&lt;p&gt;Match the carbohydrates in Column $\text{I}$ with the correct glycosidic linkages involved in Column $\text{II}$.&lt;/p&gt;&lt;p&gt;\[&lt;br&gt;\begin{array}{|l|l|}&lt;br&gt;\hline&lt;br&gt;\text{Column I} &amp;amp; \text{Column II} \\&lt;br&gt;\hline&lt;br&gt;\text{P. Amylose} &amp;amp; 1.\ \beta(1 \rightarrow 4) \\&lt;br&gt;\hline&lt;br&gt;\text{Q. Sucrose} &amp;amp; 2.\ \alpha(1 \rightarrow 4) \\&lt;br&gt;\hline&lt;br&gt;\text{R. Amylopectin} &amp;amp; 3.\ \alpha 1 \rightarrow \beta 2 \\&lt;br&gt;\hline&lt;br&gt;\text{S. Cellulose} &amp;amp; 4.\ \alpha(1 \rightarrow 4),\ \alpha(1 \rightarrow 6) \\&lt;br&gt;\hline&lt;br&gt;\end{array}&lt;br&gt;\]&lt;/p&gt;&lt;ol start=&quot;1&quot; style=&quot;list-style-type: upper-alpha;&quot;&gt;&lt;li&gt;$\text{P-4; Q-3; R-2; S-1}$&lt;/li&gt;&lt;li&gt;$\text{P-2; Q-1; R-4; S-3}$&lt;/li&gt;&lt;li&gt;$\text{P-2; Q-3; R-4; S-1}$&lt;/li&gt;&lt;li&gt;$\text{P-1; Q-3; R-4; S-2}$&lt;/li&gt;&lt;/ol&gt;</description>
<category>Biochemistry</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1308/gate-bt-2026-question-13</guid>
<pubDate>Mon, 23 Feb 2026 14:21:59 +0000</pubDate>
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<title>GATE BT 2026 | Question: 20</title>
<link>https://bt.gateoverflow.in/1301/gate-bt-2026-question-20</link>
<description>&lt;p&gt;BLAST and its extensions can be used to determine similarity of a query protein sequence with which of the following database(s)?&lt;/p&gt;&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;&lt;li&gt;A database of translated nucleotide sequences&lt;/li&gt;&lt;li&gt;A database of binding ligands and small translated peptides&lt;/li&gt;&lt;li&gt;A database of protein structures&lt;/li&gt;&lt;li&gt;A database of binding, linking and self-assembling proteins&lt;/li&gt;&lt;/ol&gt;</description>
<category>Bioinformatics</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1301/gate-bt-2026-question-20</guid>
<pubDate>Mon, 23 Feb 2026 14:21:39 +0000</pubDate>
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<title>GATE BT 2026 | Question: 22</title>
<link>https://bt.gateoverflow.in/1299/gate-bt-2026-question-22</link>
<description>You are characterizing a new enzyme isolated and purified in the laboratory. If the maximum velocity of the enzyme is $1800 ~\mu$ moles $\mathrm{L}^{-1} \mathrm{~min}^{-1}$ and the total concentration of the enzyme in the reaction mixture is $1.5 ~\mu \mathrm{M}$, then the turnover number of the enzyme is $\_\_\_\_$ $\mathrm{s}^{-1}$. (answer in integer)</description>
<category>Biochemistry</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1299/gate-bt-2026-question-22</guid>
<pubDate>Mon, 23 Feb 2026 14:21:36 +0000</pubDate>
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<item>
<title>GATE BT 2026 | Question: 23</title>
<link>https://bt.gateoverflow.in/1298/gate-bt-2026-question-23</link>
<description>To determine the viable cell count of a bacterial culture, you have plated $50 ~\mu \mathrm{~L}$ of a $100$ -fold diluted sample of the culture on a nutrient agar plate and obtained $20$ colonies after overnight incubation. The viable cell count of the culture is $\_\_\_\_$ $\mathrm{CFU} \mathrm{mL}^{-1}$. (answer in integer)</description>
<category>Microbiology</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1298/gate-bt-2026-question-23</guid>
<pubDate>Mon, 23 Feb 2026 14:21:35 +0000</pubDate>
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<title>GATE BT 2026 | Question: 25</title>
<link>https://bt.gateoverflow.in/1296/gate-bt-2026-question-25</link>
<description>You have purified an enzyme using a series of chromatographic methods. It was observed that a $10 ~\mu \mathrm{g} \mathrm{~mL}^{-1}$ of this purified enzyme converted $10$ mM substrate per hour at $25^{\circ} \mathrm{C}$ and pH $7$. Its specific activity is $\_\_\_\_$ $\mathrm{IU} \mu_{\mathrm{g}}{ }^{-1}$. (rounded off to three decimal places)</description>
<category>Analytical Techniques</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1296/gate-bt-2026-question-25</guid>
<pubDate>Mon, 23 Feb 2026 14:21:31 +0000</pubDate>
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<title>GATE BT 2026 | Question: 27</title>
<link>https://bt.gateoverflow.in/1294/gate-bt-2026-question-27</link>
<description>&lt;p&gt;Match the immunological terms in $\textbf{Column I}$ with their function/description in the $\textbf{Column II}$&lt;/p&gt;&lt;p&gt;\[&lt;br&gt;\begin{array}{|l|l|}&lt;br&gt;\hline&lt;br&gt;\textbf{Column I} &amp;amp; \textbf{Column II} \\&lt;br&gt;\hline&lt;br&gt;\text{P. } J\ \text{gene} &amp;amp; 1.\ \text{Suppresses immune system by blocking calcineurin} \\&lt;br&gt;\hline&lt;br&gt;\text{Q. Cyclosporin} &amp;amp; 2.\ \text{Antibody binding site on a large molecule} \\&lt;br&gt;\hline&lt;br&gt;\text{R. Epitope} &amp;amp; 3.\ \text{Small foreign molecule which elicits an immune response only} \\&lt;br&gt;&amp;amp; \text{when combined with a carrier molecule} \\&lt;br&gt;\hline&lt;br&gt;\text{S. Hapten} &amp;amp; 4.\ \text{Contributes to antibody diversity} \\&lt;br&gt;\hline&lt;br&gt;\end{array}&lt;br&gt;\]&lt;/p&gt;&lt;ol start=&quot;1&quot; style=&quot;list-style-type: upper-alpha;&quot;&gt;&lt;li&gt;$\text{P-3; Q-4; R-2; S-1}$&lt;/li&gt;&lt;li&gt;$\text{P-2; Q-4; R-1; S-3}$&lt;/li&gt;&lt;li&gt;$\text{P-4; Q-1; R-2; S-3}$&lt;/li&gt;&lt;li&gt;$\text{P-1; Q-3; R-4; S-2}$&lt;/li&gt;&lt;/ol&gt;</description>
<category>Immunology</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1294/gate-bt-2026-question-27</guid>
<pubDate>Mon, 23 Feb 2026 14:21:28 +0000</pubDate>
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<item>
<title>GATE BT 2026 | Question: 29</title>
<link>https://bt.gateoverflow.in/1292/gate-bt-2026-question-29</link>
<description>&lt;p&gt;Match the biomolecules in Column $\text{I}$ with their function given in Column $\text{II}$&lt;/p&gt;&lt;p&gt;\[&lt;br&gt;\begin{array}{|l|l|}&lt;br&gt;\hline&lt;br&gt;\text{Column I} &amp;amp; \text{Column II} \\&lt;br&gt;\hline&lt;br&gt;\text{P. Lectins} &amp;amp; 1.\ \text{Acts as ATP dependent pump to efflux out small molecules} \\&lt;br&gt;\hline&lt;br&gt;\text{Q. P-glycoprotein} &amp;amp; 2.\ \text{Targets lysosomal enzymes to their destination} \\&lt;br&gt;\hline&lt;br&gt;\text{R. Digitoxigenin} &amp;amp; 3.\ \text{Specific carbohydrate binding proteins} \\&lt;br&gt;\hline&lt;br&gt;\text{S. Mannose 6-phosphate} &amp;amp; 4.\ \text{Inhibits }\mathrm{Na}^{+}\!-\!\mathrm{K}^{+}\text{ pump} \\&lt;br&gt;\hline&lt;br&gt;\end{array}&lt;br&gt;\]&lt;/p&gt;&lt;ol start=&quot;1&quot; style=&quot;list-style-type: upper-alpha;&quot;&gt;&lt;li&gt;$\text{P-3; Q-2; R-1; S-4}$&lt;/li&gt;&lt;li&gt;$\text{P-1; Q-3; R-4; S-2}$&lt;/li&gt;&lt;li&gt;$\text{P-2; Q-1; R-3; S-4}$&lt;/li&gt;&lt;li&gt;$\text{P-3; Q-1; R-4; S-2}$&lt;/li&gt;&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1292/gate-bt-2026-question-29</guid>
<pubDate>Mon, 23 Feb 2026 14:21:19 +0000</pubDate>
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<title>GATE BT 2026 | Question: 30</title>
<link>https://bt.gateoverflow.in/1291/gate-bt-2026-question-30</link>
<description>&lt;p&gt;By elemental analysis of a biomass sample, it was found that the cells in the given sample are composed of (by weight) $\mathrm{C}=50 \%, \mathrm{H}=8 \%, \mathrm{O}=20 \%, \mathrm{~N}=10 \%$ and ash content $=12 \%$. Which of the following is the correct empirical formula of the ashfree cells, normalized with respect to Nitrogen?&lt;/p&gt;&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;&lt;li&gt;$\mathrm{C}_{5.8} \mathrm{H}_{11.2} \mathrm{O}_{1.7} \mathrm{~N}$&lt;/li&gt;&lt;li&gt;$\mathrm{C}_{5.0} \mathrm{H}_{10.2} \mathrm{O}_{1.1} \mathrm{~N}$&lt;/li&gt;&lt;li&gt;$\mathrm{C}_{6.8} \mathrm{H}_{12.2} \mathrm{O}_{2.1} \mathrm{~N}$&lt;/li&gt;&lt;li&gt;$\mathrm{C}_{4.8} \mathrm{H}_{9.2} \mathrm{O}_{3.1} \mathrm{~N}$&lt;/li&gt;&lt;/ol&gt;</description>
<category>Biochemistry</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1291/gate-bt-2026-question-30</guid>
<pubDate>Mon, 23 Feb 2026 14:21:14 +0000</pubDate>
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<title>GATE BT 2026 | Question: 32</title>
<link>https://bt.gateoverflow.in/1289/gate-bt-2026-question-32</link>
<description>&lt;p&gt;Messenger $\text{RNAs (mRNAs)}$ translate to generate polypeptides and the translation terminates at the $\text{UGA, UAG}$ or $\text{UAA}$ stop codons. The $\text{AGA}$ codon acts as additional stop codon in the mitochondria. The longest possible polypeptide(s) that can be hypothetically translated in the cytosol and/or mitochondria, from the given full length $\text{mRNA}$ sequence ($36$ nucleotides) is/are&lt;/p&gt;&lt;p&gt;$\text{5&#039;-AACACCAUGACCCAUGUGGCGAGACGGUAGUUAAAA- 3&#039;}$&lt;/p&gt;&lt;ol start=&quot;1&quot; style=&quot;list-style-type: upper-alpha;&quot;&gt;&lt;li&gt;$7$ amino acids long in the cytosol&lt;/li&gt;&lt;li&gt;$5$ amino acids long in the mitochondria&lt;/li&gt;&lt;li&gt;$6$ amino acids long in the mitochondria&lt;/li&gt;&lt;li&gt;$8$ amino acids long in the cytosol&lt;/li&gt;&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1289/gate-bt-2026-question-32</guid>
<pubDate>Mon, 23 Feb 2026 14:21:08 +0000</pubDate>
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<title>GATE BT 2026 | Question: 33</title>
<link>https://bt.gateoverflow.in/1288/gate-bt-2026-question-33</link>
<description>&lt;p&gt;The RNA sequence below depicts the part of a $330$ nucleotides long $\text{mRNA}$ and it encodes the $\text{C}$-terminal portion of a protein.&lt;/p&gt;&lt;p&gt;$5^{\prime}-\ldots \ldots$ $\text{AAC ACC ACG ACC CAU GUG GCG AGA CGG UAG}$ - $3^{\prime}$&lt;/p&gt;&lt;p&gt;A mutation was identified in this $\text{RNA}$ denoted as $322 \mathrm{~A} \rightarrow \mathrm{U}$. This nucleotide change is represented by which of the following class(es) of mutation?&lt;/p&gt;&lt;ol start=&quot;1&quot; style=&quot;list-style-type: upper-alpha;&quot;&gt;&lt;li&gt;Missense mutation&lt;/li&gt;&lt;li&gt;Non-sense mutation&lt;/li&gt;&lt;li&gt;Transversion mutation&lt;/li&gt;&lt;li&gt;Silent mutation&lt;/li&gt;&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1288/gate-bt-2026-question-33</guid>
<pubDate>Mon, 23 Feb 2026 14:21:05 +0000</pubDate>
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<title>GATE BT 2026 | Question: 38</title>
<link>https://bt.gateoverflow.in/1283/gate-bt-2026-question-38</link>
<description>&lt;p&gt;Which of the following is/are posttranslational modification(s) involved in epigenetic control of gene expression?&lt;/p&gt;&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;&lt;li&gt;Arginine methylation&lt;/li&gt;&lt;li&gt;Lysine acetylation&lt;/li&gt;&lt;li&gt;Cytosine methylation&lt;/li&gt;&lt;li&gt;Cytosine deamination&lt;/li&gt;&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1283/gate-bt-2026-question-38</guid>
<pubDate>Mon, 23 Feb 2026 14:20:51 +0000</pubDate>
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<title>GATE BT 2026 | Question: 47</title>
<link>https://bt.gateoverflow.in/1274/gate-bt-2026-question-47</link>
<description>The electron equivalent per liter (e-eq $\mathrm{L}^{-1}$ ) of acetate ion solution of concentration $10 \mathrm{~g} \mathrm{~L}^{-1}$ is $\_\_\_\_$ $\mathrm{e}^{-} \mathrm{eq} \mathrm{L}^{-1}$. (rounded off to one decimal place)</description>
<category>Analytical Techniques</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1274/gate-bt-2026-question-47</guid>
<pubDate>Mon, 23 Feb 2026 14:20:33 +0000</pubDate>
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<title>GATE BT 2026 | Question: 53</title>
<link>https://bt.gateoverflow.in/1268/gate-bt-2026-question-53</link>
<description>In the process of generating a clonal mammalian cell line, a single cell was seeded in a well of a cell culture plate. After the first $48$ hours, one of the progeny cells underwent apoptosis due to a new mutation. If the doubling time of the cells is $24$ hours and no more cell death occurs, the total number of cells after a total of $7$ days from seeding will be $\_\_\_\_$. (answer in integer)</description>
<category>Cell Biology</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1268/gate-bt-2026-question-53</guid>
<pubDate>Mon, 23 Feb 2026 14:20:26 +0000</pubDate>
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<title>GATE BT 2025 | Question: 5</title>
<link>https://bt.gateoverflow.in/1074/gate-bt-2025-question-5</link>
<description>&lt;p&gt;​​​​​The diversity in $T$-cell receptors is generated by&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;gene rearrangements&lt;/li&gt;
	&lt;li&gt;somatic hypermutation of rearranged $V$ region&lt;/li&gt;
	&lt;li&gt;gene conversion&lt;/li&gt;
	&lt;li&gt;class switching&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Immunology</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1074/gate-bt-2025-question-5</guid>
<pubDate>Thu, 06 Mar 2025 17:06:11 +0000</pubDate>
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<title>GATE BT 2025 | Question: 6</title>
<link>https://bt.gateoverflow.in/1073/gate-bt-2025-question-6</link>
<description>&lt;p&gt;​​​​Which one of the following is true for piRNAs?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;piRNAs silence transposable elements in germ cells&lt;/li&gt;
	&lt;li&gt;piRNA is the abbreviation of $P$-element interacting RNA&lt;/li&gt;
	&lt;li&gt;piRNAs modify the $2^{\prime}-\mathrm{OH}$ of ribose with methyl group&lt;/li&gt;
	&lt;li&gt;piRNA is a long non-coding RNA&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1073/gate-bt-2025-question-6</guid>
<pubDate>Thu, 06 Mar 2025 17:06:09 +0000</pubDate>
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<title>GATE BT 2025 | Question: 7</title>
<link>https://bt.gateoverflow.in/1072/gate-bt-2025-question-7</link>
<description>&lt;p&gt;​​​​Which one of the following coenzymes is utilised by alanine racemase for the conversion of $L$-Alanine to $D$-Alanine?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;Pyridoxal phosphate&lt;/li&gt;
	&lt;li&gt;Thiamine pyrophosphate&lt;/li&gt;
	&lt;li&gt;Tetrahydrofolate&lt;/li&gt;
	&lt;li&gt;Flavin mononucleotide&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Biochemistry</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1072/gate-bt-2025-question-7</guid>
<pubDate>Thu, 06 Mar 2025 17:06:06 +0000</pubDate>
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<title>GATE BT 2025 | Question: 12</title>
<link>https://bt.gateoverflow.in/1067/gate-bt-2025-question-12</link>
<description>&lt;p&gt;​​​​Which of the following enzymes is/are involved in the biogenesis of miRNA?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;Drosha&lt;/li&gt;
	&lt;li&gt;Cas$9$&lt;/li&gt;
	&lt;li&gt;XRCC$4$&lt;/li&gt;
	&lt;li&gt;Dicer&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1067/gate-bt-2025-question-12</guid>
<pubDate>Thu, 06 Mar 2025 17:05:53 +0000</pubDate>
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<title>GATE BT 2025 | Question: 13</title>
<link>https://bt.gateoverflow.in/1066/gate-bt-2025-question-13</link>
<description>&lt;p&gt;​​​​​​Which of the following separation processes is/are based on molecular size?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;Size-exclusion chromatography&lt;/li&gt;
	&lt;li&gt;Ion exchange chromatography&lt;/li&gt;
	&lt;li&gt;Membrane ultrafiltration&lt;/li&gt;
	&lt;li&gt;Ultracentrifugation&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Analytical Techniques</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1066/gate-bt-2025-question-13</guid>
<pubDate>Thu, 06 Mar 2025 17:05:52 +0000</pubDate>
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<title>GATE BT 2025 | Question: 14</title>
<link>https://bt.gateoverflow.in/1065/gate-bt-2025-question-14</link>
<description>&lt;p&gt;​​Which of the following show(s) optical activity at $100 \: \mathrm{mM}$ concentration in water?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;Solution of NaCl&lt;/li&gt;
	&lt;li&gt;Solution of D-Glucose&lt;/li&gt;
	&lt;li&gt;Solution of Glycine&lt;/li&gt;
	&lt;li&gt;Solution of L-Proline&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Biochemistry</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1065/gate-bt-2025-question-14</guid>
<pubDate>Thu, 06 Mar 2025 17:05:49 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 16</title>
<link>https://bt.gateoverflow.in/1063/gate-bt-2025-question-16</link>
<description>&lt;h2 style=&quot;font-style:italic;&quot;&gt;&amp;nbsp;&lt;/h2&gt;

&lt;p&gt;​​​Which of the following compounds have the same degree of reduction per carbon-mole?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;Glucose&lt;/li&gt;
	&lt;li&gt;Lactic acid&lt;/li&gt;
	&lt;li&gt;Acetic acid&lt;/li&gt;
	&lt;li&gt;Formic acid&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Biochemistry</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1063/gate-bt-2025-question-16</guid>
<pubDate>Thu, 06 Mar 2025 17:05:45 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 21</title>
<link>https://bt.gateoverflow.in/1058/gate-bt-2025-question-21</link>
<description>The contour length of a B-DNA molecule that encodes a bacterial protein of $33 \: \text{kDa}$ is $\_\_\_\_$ $\mathrm{ nm}$.&lt;br /&gt;
&lt;br /&gt;
Consider the average molecular weight of an amino acid as $110 \: \text{Da}$ and helix rise per base pair for B-DNA as $0.34 \: nm$.&lt;br /&gt;
(Round off to the nearest integer)</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1058/gate-bt-2025-question-21</guid>
<pubDate>Thu, 06 Mar 2025 17:05:37 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 22</title>
<link>https://bt.gateoverflow.in/1057/gate-bt-2025-question-22</link>
<description>Within the Michaelis-Menten framework, the ratio of $v_{0} / V_{\max }$ when $[\mathrm{S}]=20 \times K_{\mathrm{m}}$ is $\_\_\_\_\_$&lt;br /&gt;
&lt;br /&gt;
(Round off to two decimal places)</description>
<category>Analytical Techniques</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1057/gate-bt-2025-question-22</guid>
<pubDate>Thu, 06 Mar 2025 17:05:36 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 27</title>
<link>https://bt.gateoverflow.in/1052/gate-bt-2025-question-27</link>
<description>&lt;p&gt;Correctly match the &lt;strong&gt;Inhibitor &lt;/strong&gt;with its respective &lt;strong&gt;Function &lt;/strong&gt;in mitochondrial respiration.&lt;/p&gt;

&lt;p&gt;\[&lt;br&gt;
\begin{array}{|l|}&lt;br&gt;
&lt;br&gt;
\hline&lt;br&gt;
\begin{array}{l|l}&lt;br&gt;
\textbf{Inhibitor} &amp;amp; \textbf{Function}&amp;nbsp;\\&amp;nbsp;&lt;br&gt;
\hline&lt;br&gt;
\text{P. FCCP} &amp;amp; \text{1.&amp;nbsp;Inhibits cytochrome c oxidase } \\&amp;nbsp; \hline&lt;br&gt;
\text{Q. Cyanide} &amp;amp; \text{2. Makes the membrane permeable of protons}\\&amp;nbsp; \hline&lt;br&gt;
\text{R. Oligomycin A} &amp;amp; \text{3.&amp;nbsp;Blocks mitochondrial uptake of succinate} \\&amp;nbsp; \hline&lt;br&gt;
\text{S. Butyl malonates} &amp;amp; \text{4. Inhibits ATP synthase} \\&amp;nbsp; \hline&lt;br&gt;
\end{array} \\&lt;br&gt;
\end{array}&lt;br&gt;
\]&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;$\mathrm{P}-2 ; \mathrm{Q}-1 ; \mathrm{R}-4 ; \mathrm{S}-3$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-2 ; \mathrm{Q}-3 ; \mathrm{R}-1 ; \mathrm{S}-4$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-2 ; \mathrm{Q}-4 ; \mathrm{R}-3 ; \mathrm{S}-1$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-3 ; \mathrm{Q}-1 ; \mathrm{R}-2 ; \mathrm{S}-4$&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Biochemistry</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1052/gate-bt-2025-question-27</guid>
<pubDate>Thu, 06 Mar 2025 17:05:29 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 28</title>
<link>https://bt.gateoverflow.in/1051/gate-bt-2025-question-28</link>
<description>&lt;p&gt;​​​​​An octapeptide composed of these $L$-amino acids - Lys, Thr, Ser, Met, Arg, Trp, Tyr, Glu - was subjected to analyses with the following outcomes:&lt;/p&gt;

&lt;p&gt;$\text{P}$. The N-terminal sequencing analysis by Sanger&#039;s method yielded &#039;Ser&#039; at the $N$ -terminus.&lt;/p&gt;

&lt;p&gt;$\text{Q}$. Chymotrypsin treatment gave a pentapeptide, a &#039;Tyr&#039; containing dipeptide and a free &#039;Glu&#039;.&lt;/p&gt;

&lt;p&gt;$\text{R}$. Cyanogen bromide treatment gave two tetrapeptides.&lt;/p&gt;

&lt;p&gt;$\text{S}$. Trypsin treatment gave two tripeptides and a dipeptide&lt;/p&gt;

&lt;p&gt;Which one of the following is the correct octapeptide sequence?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;Ser-Tyr-Arg-Met-Lys-Thr-Trp-Glu&lt;/li&gt;
	&lt;li&gt;Ser-Arg-Lys-Met-Tyr-Thr-Trp-Glu&lt;/li&gt;
	&lt;li&gt;Ser-Met-Lys-Arg-Thr-Tyr-Trp-Glu&lt;/li&gt;
	&lt;li&gt;Ser-Arg-Met-Lys-Trp-Thr-Tyr-Glu&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1051/gate-bt-2025-question-28</guid>
<pubDate>Thu, 06 Mar 2025 17:05:27 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 29</title>
<link>https://bt.gateoverflow.in/1050/gate-bt-2025-question-29</link>
<description>&lt;p&gt;​​​Correctly match the type of &lt;strong&gt;Hypersensitivity reaction &lt;/strong&gt;with its respective &lt;strong&gt;Example&lt;/strong&gt;.&lt;br&gt;
$$\begin{array}{|l|l|}&lt;br&gt;
\hline&amp;nbsp;\textbf{Hypersensitivity reaction} &amp;amp; \textbf{Example} \\&lt;br&gt;
\hline&amp;nbsp;\text{P. Type I} &amp;amp; 1.&amp;nbsp;\text{Tuberculin reaction} \\&lt;br&gt;
\hline&amp;nbsp;\text{Q. Type II}&amp;nbsp;&amp;amp; 2. \text{Arthus reaction} \\&lt;br&gt;
\hline&amp;nbsp;\text{R. Type III} &amp;amp; 3. \text{Chronic urticaria} \\&lt;br&gt;
\hline&amp;nbsp;\text{S. Type IV} &amp;amp; 4. \text{Systemic anaphylaxis} \\&lt;br&gt;
\hline&lt;br&gt;
\end{array}$$&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;$\mathrm{P}-3 ; \mathrm{Q}-4 ; \mathrm{R}-2 ; \mathrm{S}-1$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-4 ; \mathrm{Q}-3 ; \mathrm{R}-1 ; \mathrm{S}-2$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-4 ; \mathrm{Q}-3 ; \mathrm{R}-2 ; \mathrm{S}-1$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-2 ; \mathrm{Q}-3 ; \mathrm{R}-4 ; \mathrm{S}-1$&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Immunology</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1050/gate-bt-2025-question-29</guid>
<pubDate>Thu, 06 Mar 2025 17:05:23 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 30</title>
<link>https://bt.gateoverflow.in/1049/gate-bt-2025-question-30</link>
<description>&lt;p&gt;Correctly match the &lt;strong&gt;Enzyme &lt;/strong&gt;with its respective &lt;strong&gt;Function&lt;/strong&gt;.&lt;br&gt;
\begin{array}{|l|l|}&lt;br&gt;
\hline \textbf{ Enzyme } &amp;amp; \textbf{ Function } \\&lt;br&gt;
\hline&amp;nbsp;\text{P. Gyrase} &amp;amp; 1. \text{Removes a damaged base} \\ &amp;amp; \text{by cleaving the bond} \\ &amp;amp;&lt;br&gt;
\text{between sugar and base}&amp;nbsp;\\&lt;br&gt;
\hline&amp;nbsp;\text{Q. Deadenylase} &amp;amp;&amp;nbsp;2. \text{Provides a swivel allowing one DNA} \\ &amp;amp; \text{strand to rotate around the other} \\&lt;br&gt;
\hline&amp;nbsp;\text{R. Glycosylase} &amp;amp;&amp;nbsp;3. \text{Catalyses bond formation between 3&#039;-OH} \\ &amp;amp;&amp;nbsp; \text{and 5&#039;-phosphate end of nucleotides in duplex DNA}&amp;nbsp;&amp;nbsp;\\&lt;br&gt;
\hline&amp;nbsp;\text{S. DNA ligase} &amp;amp;&amp;nbsp;4. \text{Is an exoribonuclease that removes} \\ &amp;amp; \text{the poly(A) tail} \\&amp;nbsp; \hline&lt;br&gt;
\end{array}&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;$\mathrm{P}-2 ; \mathrm{Q}-4 ; \mathrm{R}-1 ; \mathrm{S}-3$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-1 ; \mathrm{Q}-4 ; \mathrm{R}-2 ; \mathrm{S}-3$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-2 ; \mathrm{Q}-1 ; \mathrm{R}-4 ; \mathrm{S}-3$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-3 ; \mathrm{Q}-2 ; \mathrm{R}-1 ; \mathrm{S}-4$&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1049/gate-bt-2025-question-30</guid>
<pubDate>Thu, 06 Mar 2025 17:05:20 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 31</title>
<link>https://bt.gateoverflow.in/1048/gate-bt-2025-question-31</link>
<description>&lt;p&gt;​​​​Correctly match the &lt;strong&gt;Coenzyme &lt;/strong&gt;with its respective involvement in a specific &lt;strong&gt;Reaction type&lt;/strong&gt;.&lt;br&gt;
\begin{array}{|l|l|}&lt;br&gt;
\hline \textbf{Coenzyme } &amp;amp; \textbf{Reaction type} \\&lt;br&gt;
\hline&amp;nbsp;\text{P. Thiamine pyrophosphate} &amp;amp; 1. \text{Acyl group transfer} \\&lt;br&gt;
\hline&amp;nbsp;\text{Q. Tetrahydrofolate} &amp;amp; 2. \text{Transfer of one carbon group} \\&lt;br&gt;
\hline&amp;nbsp;\text{R. Flavin adenine dinucleotide} &amp;amp; 3. \text{Transfer of methyl group} \\&lt;br&gt;
\hline S. \text{5&#039;-Deoxyadenosyl cobalamin} &amp;amp; 4. \text{Oxidation-reduction} \\&lt;br&gt;
\hline &amp;amp; 5. \text{Aldehyde transfer} \\&lt;br&gt;
\hline&lt;br&gt;
\end{array}&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;$\mathrm{P}-5 ; \mathrm{Q}-2 ; \mathrm{R}-4 ; \mathrm{S}-3$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-5 ; \mathrm{Q}-1 ; \mathrm{R}-2 ; \mathrm{S}-3$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-1 ; \mathrm{Q}-2 ; \mathrm{R}-4 ; \mathrm{S}-5$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-5 ; \mathrm{Q}-3 ; \mathrm{R}-1 ; \mathrm{S}-2$&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Biochemistry</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1048/gate-bt-2025-question-31</guid>
<pubDate>Thu, 06 Mar 2025 17:05:17 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 37</title>
<link>https://bt.gateoverflow.in/1042/gate-bt-2025-question-37</link>
<description>&lt;p&gt;​​​​Correctly match the following &lt;strong&gt;Bioinformatic tool/Database &lt;/strong&gt;with its respective &lt;strong&gt;Utility&lt;/strong&gt;.&lt;/p&gt;

&lt;p&gt;\begin{array}{|l|l|}&lt;br&gt;
\hline&amp;nbsp;\textbf{Bioinformatic tool/Database} &amp;amp; \textbf{Utility} \\&lt;br&gt;
\hline&amp;nbsp;\text{P. BLAST} &amp;amp; 1. \text{Database for 3D protein structures} \\&lt;br&gt;
\hline&amp;nbsp;\text{Q. Bowtie} &amp;amp; 2. \text{Tool to identify similarity of a query} \\ &amp;amp; \text{sequence to existing sequences} \\ &amp;amp; \text{ available&amp;nbsp;in databanks}&amp;nbsp;\\&lt;br&gt;
\hline&amp;nbsp;\text{R. AlphaFold} &amp;amp;&amp;nbsp;3. \text{Tool to align short read DNA sequences} \\ &amp;amp;&lt;br&gt;
\text{obtained from Next-generation} \\ &amp;amp; \text{sequencing to a reference genome} \\&lt;br&gt;
\hline&amp;nbsp;\text{S. PDB} &amp;amp; 4. \text{AI tool to predict protein structures} \\ &amp;nbsp;\hline&lt;br&gt;
\end{array}&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;$\mathrm{P}-2 ; \mathrm{Q}-3 ; \mathrm{R}-1 ; \mathrm{S}-4$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-2 ; \mathrm{Q}-3 ; \mathrm{R}-4 ; \mathrm{S}-1$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-3 ; \mathrm{Q}-2 ; \mathrm{R}-4 ; \mathrm{S}-1$&lt;/li&gt;
	&lt;li&gt;$\mathrm{P}-4 ; \mathrm{Q}-1 ; \mathrm{R}-2 ; \mathrm{S}-3$&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Bioinformatics</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1042/gate-bt-2025-question-37</guid>
<pubDate>Thu, 06 Mar 2025 17:05:00 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 39</title>
<link>https://bt.gateoverflow.in/1040/gate-bt-2025-question-39</link>
<description>&lt;p&gt;​​​​​Which of the following statements is/are true regarding the effect of the concentration of metabolic intermediates on glycolysis in erythrocytes?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;Increased AMP levels stimulate glycolysis&lt;/li&gt;
	&lt;li&gt;Increased citrate inhibits glycolysis&lt;/li&gt;
	&lt;li&gt;Increased glucose $6$-phosphate inhibits glycolysis&lt;/li&gt;
	&lt;li&gt;Increased fructose $1,6$-bisphosphate stimulates glycolysis&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Biochemistry</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1040/gate-bt-2025-question-39</guid>
<pubDate>Thu, 06 Mar 2025 17:04:54 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 40</title>
<link>https://bt.gateoverflow.in/1039/gate-bt-2025-question-40</link>
<description>&lt;p&gt;​​​​Which of the following statements about initiation of DNA replication in eukaryotes is/are true?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;DNA replication is initiated at the origin of replication licensed by loading of Mcm helicase complex&lt;/li&gt;
	&lt;li&gt;Loading of Mcm helicase complex takes place in S phase&lt;/li&gt;
	&lt;li&gt;Mcm helicase complex are activated by S-Cdks&lt;/li&gt;
	&lt;li&gt;Mcm helicase complex is responsible for loading of origin recognition complex&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1039/gate-bt-2025-question-40</guid>
<pubDate>Thu, 06 Mar 2025 17:04:51 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 41</title>
<link>https://bt.gateoverflow.in/1038/gate-bt-2025-question-41</link>
<description>&lt;p&gt;​​​​Which of the following proteins is/are involved in intraflagellar transport?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;Microtubules&lt;/li&gt;
	&lt;li&gt;Myosin&lt;/li&gt;
	&lt;li&gt;Actin&lt;/li&gt;
	&lt;li&gt;Kinesin&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1038/gate-bt-2025-question-41</guid>
<pubDate>Thu, 06 Mar 2025 17:04:49 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 42</title>
<link>https://bt.gateoverflow.in/1037/gate-bt-2025-question-42</link>
<description>&lt;p&gt;​​​​Which of the following statements is/are true about telomerase?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;Telomerase has $5^{\prime}-3^{\prime}$ DNA-dependent DNA polymerisation activity&lt;/li&gt;
	&lt;li&gt;Telomerase has $5^{\prime}-3^{\prime}$ RNA-dependent DNA polymerisation activity&lt;/li&gt;
	&lt;li&gt;Telomerase contains an RNA subunit&lt;/li&gt;
	&lt;li&gt;Telomerase has $3^{\prime}-5^{\prime}$ DNA-dependent DNA polymerisation activity&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1037/gate-bt-2025-question-42</guid>
<pubDate>Thu, 06 Mar 2025 17:04:47 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 46</title>
<link>https://bt.gateoverflow.in/1033/gate-bt-2025-question-46</link>
<description>&lt;p&gt;​​​​​Which of the following amino acids contain(s) two chiral carbons?&lt;/p&gt;

&lt;ol start=&quot;1&quot; style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;L-Leucine&lt;/li&gt;
	&lt;li&gt;L-Threonine&lt;/li&gt;
	&lt;li&gt;L-Isoleucine&lt;/li&gt;
	&lt;li&gt;L-Asparagine&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Biochemistry</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1033/gate-bt-2025-question-46</guid>
<pubDate>Thu, 06 Mar 2025 17:04:38 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 50</title>
<link>https://bt.gateoverflow.in/1029/gate-bt-2025-question-50</link>
<description>The percentage of light that would pass through a sample with an absorbance of $2$ would be $\_\_\_\_\_$ $\%$ .&lt;br /&gt;
&lt;br /&gt;
(Round off to the nearest integer)</description>
<category>Analytical Techniques</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1029/gate-bt-2025-question-50</guid>
<pubDate>Thu, 06 Mar 2025 17:04:30 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 54</title>
<link>https://bt.gateoverflow.in/1025/gate-bt-2025-question-54</link>
<description>An NMR spectrometer operating at proton resonance frequency $(v)$ of $1 \: \mathrm{GHz}$ will have a magnetic field strength of $\_\_\_\_$ Tesla (T).&lt;br /&gt;
&lt;br /&gt;
The gyromagnetic ratio for proton, $\gamma=2.675 \times 10^{8} \mathrm{~T}^{-1} \mathrm{~s}^{-1}$&lt;br /&gt;
(Round off to one decimal place)</description>
<category>Analytical Techniques</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1025/gate-bt-2025-question-54</guid>
<pubDate>Thu, 06 Mar 2025 17:04:25 +0000</pubDate>
</item>
<item>
<title>GATE BT 2025 | Question: 55</title>
<link>https://bt.gateoverflow.in/1024/gate-bt-2025-question-55</link>
<description>For the coupled reactions given below&lt;br /&gt;
\[&lt;br /&gt;
\begin{array}{ll}&lt;br /&gt;
\text { Glucose 6-phosphate }+\mathrm{H}_{2} \mathrm{O} \rightarrow \text { Glucose }+\mathrm{Pi} &amp;amp; \text { (Reaction 1) } \\&lt;br /&gt;
\text { ATP }+ \text { Glucose } \rightarrow \mathrm{ADP}+\text { Glucose 6-phosphate } &amp;amp; \text { (Reaction 2) }&lt;br /&gt;
\end{array}&lt;br /&gt;
\]&lt;br /&gt;
the standard free energy change of ATP hydrolysis at $25^{\circ} \mathrm{C}$ is $\qquad$ $\mathrm{kJ} / \mathrm{mol}$.&lt;br /&gt;
&lt;br /&gt;
The equilibrium constants for Reaction 1 and Reaction 2 are 360 and 800, respectively; Gas constant $R=8.314 \mathrm{~J} \mathrm{~mol}^{-1} \mathrm{~K}^{-1}$.&lt;br /&gt;
(Round off to two decimal places)</description>
<category>Analytical Techniques</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1024/gate-bt-2025-question-55</guid>
<pubDate>Thu, 06 Mar 2025 17:04:24 +0000</pubDate>
</item>
<item>
<title>GATE BT 2024 | Question: 1</title>
<link>https://bt.gateoverflow.in/1012/gate-bt-2024-question-1</link>
<description>&lt;p&gt;In adsorption chromatography, the adsorption of uncharged solute molecules onto a silica-based stationary phase is by $\_\_\_\_\_\_\_$.&lt;/p&gt;

&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;covalent bonds&lt;/li&gt;
	&lt;li&gt;electrostatic interactions&amp;nbsp;&lt;/li&gt;
	&lt;li&gt;ionic bonds&lt;/li&gt;
	&lt;li&gt;van der Waals forces&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Analytical Techniques</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1012/gate-bt-2024-question-1</guid>
<pubDate>Mon, 25 Mar 2024 19:16:35 +0000</pubDate>
</item>
<item>
<title>GATE BT 2024 | Question: 7</title>
<link>https://bt.gateoverflow.in/1006/gate-bt-2024-question-7</link>
<description>&lt;p&gt;Co-translational translocation of proteins is observed in $\_\_\_\_\_\_\_$.&lt;/p&gt;

&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;endoplasmic reticulum&lt;/li&gt;
	&lt;li&gt;Golgi complex&lt;/li&gt;
	&lt;li&gt;mitochondria&lt;/li&gt;
	&lt;li&gt;peroxisomes&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1006/gate-bt-2024-question-7</guid>
<pubDate>Mon, 25 Mar 2024 19:16:29 +0000</pubDate>
</item>
<item>
<title>GATE BT 2024 | Question: 8</title>
<link>https://bt.gateoverflow.in/1005/gate-bt-2024-question-8</link>
<description>&lt;p&gt;$2$-mercaptoethanol breaks the $\_\_\_\_\_\_\_$ covalent bond between light and heavy chains of an immunoglobulin molecule.&lt;/p&gt;

&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;$\text{C-N}$&amp;nbsp;&lt;/li&gt;
	&lt;li&gt;$\text{N-O}$&lt;/li&gt;
	&lt;li&gt;$\text{S-C}$&lt;/li&gt;
	&lt;li&gt;$\text{S-S}$
	&lt;p&gt;&amp;nbsp;&lt;/p&gt;
	&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Immunology</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1005/gate-bt-2024-question-8</guid>
<pubDate>Mon, 25 Mar 2024 19:16:29 +0000</pubDate>
</item>
<item>
<title>GATE BT 2024 | Question: 10</title>
<link>https://bt.gateoverflow.in/1003/gate-bt-2024-question-10</link>
<description>&lt;p&gt;A cultured skin fibroblast cell of a goat &#039; $\mathrm{P}$ &#039; was fused with an enucleated ovum of a goat &#039; $\mathrm{Q}$ &#039;. The resultant activated early embryo was then transplanted into a pseudopregnant (surrogate) female goat &#039; $\mathrm{R}$&amp;nbsp;&#039; of the same strain as &#039; $\mathrm{Q}$&amp;nbsp;&#039;. On completion of gestation, a female goat &#039; $\mathrm{S}$&amp;nbsp;&#039; was born. With the exception of mitochondrial DNA, &#039; $\mathrm{S}$&amp;nbsp;&#039; is a clone of $\_\_\_\_\_\_\_\_$.&lt;/p&gt;

&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;Only&amp;nbsp;$\mathrm{P}$&lt;/li&gt;
	&lt;li&gt;Only $\mathrm{Q}$&amp;nbsp;&lt;/li&gt;
	&lt;li&gt;Only&amp;nbsp;$\mathrm{R}$&lt;/li&gt;
	&lt;li&gt;Both $\mathrm{P}$&amp;nbsp;and&amp;nbsp;$\mathrm{R}$
	&lt;p&gt;&amp;nbsp;&lt;/p&gt;
	&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/1003/gate-bt-2024-question-10</guid>
<pubDate>Mon, 25 Mar 2024 19:16:27 +0000</pubDate>
</item>
<item>
<title>GATE BT 2024 | Question: 14</title>
<link>https://bt.gateoverflow.in/999/gate-bt-2024-question-14</link>
<description>&lt;p&gt;An element that is present in a nucleotide but not in a nucleoside is $\_\_\_\_\_\_\_$&lt;/p&gt;

&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;carbon&lt;/li&gt;
	&lt;li&gt;nitrogen&lt;/li&gt;
	&lt;li&gt;oxygen&lt;/li&gt;
	&lt;li&gt;phosphorous
	&lt;p&gt;&amp;nbsp;&lt;/p&gt;
	&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/999/gate-bt-2024-question-14</guid>
<pubDate>Mon, 25 Mar 2024 19:16:23 +0000</pubDate>
</item>
<item>
<title>GATE BT 2024 | Question: 15</title>
<link>https://bt.gateoverflow.in/998/gate-bt-2024-question-15</link>
<description>&lt;p&gt;Krebs $\text{(TCA)}$ cycle is $\_\_\_\_\_\_$&amp;nbsp;pathway.&lt;/p&gt;

&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;only an anabolic&lt;/li&gt;
	&lt;li&gt;only a catabolic&amp;nbsp;&lt;/li&gt;
	&lt;li&gt;an amphibolic&lt;/li&gt;
	&lt;li&gt;a pyogenic
	&lt;p&gt;&amp;nbsp;&lt;/p&gt;
	&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Biochemistry</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/998/gate-bt-2024-question-15</guid>
<pubDate>Mon, 25 Mar 2024 19:16:23 +0000</pubDate>
</item>
<item>
<title>GATE BT 2024 | Question: 16</title>
<link>https://bt.gateoverflow.in/997/gate-bt-2024-question-16</link>
<description>&lt;p&gt;If a denatured protein of human origin is injected into a rabbit, antibodies generated will recognize the $\_\_\_\_\_\_$ structure of the protein.&lt;/p&gt;

&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;primary&lt;/li&gt;
	&lt;li&gt;secondary&lt;/li&gt;
	&lt;li&gt;tertiary&lt;/li&gt;
	&lt;li&gt;quaternary
	&lt;p&gt;&amp;nbsp;&lt;/p&gt;
	&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/997/gate-bt-2024-question-16</guid>
<pubDate>Mon, 25 Mar 2024 19:16:22 +0000</pubDate>
</item>
<item>
<title>GATE BT 2024 | Question: 17</title>
<link>https://bt.gateoverflow.in/996/gate-bt-2024-question-17</link>
<description>&lt;p&gt;All pseudogenes &lt;strong&gt;DO NOT&lt;/strong&gt; code for a $\_\_\_\_\_\_\_$.&lt;/p&gt;

&lt;ol style=&quot;list-style-type:upper-alpha&quot;&gt;
	&lt;li&gt;protein with original function&lt;/li&gt;
	&lt;li&gt;protein with altered function&lt;/li&gt;
	&lt;li&gt;RNA with coding sequence&lt;/li&gt;
	&lt;li&gt;RNA with regulatory function
	&lt;p&gt;&amp;nbsp;&lt;/p&gt;
	&lt;/li&gt;
&lt;/ol&gt;</description>
<category>Molecular Biology and Genetics:</category>
<guid isPermaLink="true">https://bt.gateoverflow.in/996/gate-bt-2024-question-17</guid>
<pubDate>Mon, 25 Mar 2024 19:16:21 +0000</pubDate>
</item>
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