GATE Overflow for GATE BT - Questions without answers in Molecular tools

GATE BT 2025 | Question: 45

Thu, 06 Mar 2025 17:04:40 +0000

Which of the following methods is/are used for identifying histone modifications?

ChIP-seq
Mass spectrometry
Immunofluorescence
Patch-clamp electrophysiology

GATE BT 2024 | Question: 13

Mon, 25 Mar 2024 19:16:24 +0000

Which one of the following is the basic principle of Sanger's DNA sequencing method?

Chain termination by incorporation of dideoxynucleotides
Chain elongation by incorporation of dideoxynucleotides
Release of inorganic pyrophosphate
Chain cleavage by modification of dideoxynucleotides

GATE BT 2024 | Question: 28

Mon, 25 Mar 2024 19:16:16 +0000

Match the enzyme (Column I) with its corresponding function (Column II).

\begin{array}{|c|c|c|}
\hline & \text{Column I} & \text{Column II} \\
\hline & \text{P. Primase} & 1. \text{RNA dependent RNA synthesis} \\
\hline & \text{Q. Reverse transcriptase} & 2. \text{DNA dependent DNA synthesis} \\
\hline & \text{R. RNA Replicase} & 3. \text{RNA dependent DNA synthesis} \\
\hline & \text{S. DNA Polymerase III} & 4. \text{DNA dependent RNA synthesis} \\
\hline
\end{array}

$\text{P-4; Q-1; R-3; S-2}$
$\text{P-2; Q-1; R-3; S-4}$
$\text{P-3; Q-4; R-2; S-1}$
$\text{P-4; Q-3; R-1; S-2}$

GATE BT 2024 | Question: 30

Mon, 25 Mar 2024 19:16:16 +0000

Match the chemical (Column I) with its use (Column II).

\begin{array}{|c|c|c|}
\hline \text{Column I} & \text{Column II} \\
\hline \text{P. Diethylpyrocarbonate} & 1. \text{Chelation of magnesium ion during DNA purification}\\
\hline \text{Q. Cesium chloride} & 2. \text{Prevention of RNA degradation in aqueous environment}\\
\hline \text{R. Ethidium bromide} & 3. \text{Separation of DNA by density gradient centrifugation}\\
\hline \text{S. Ethylenediaminetetraacetic acid} & 4. \text{Staining of RNA in agarose gel} \\
\hline
\end{array}

$\text{P-4; Q-1; R-3; S-2}$
$\text{P-4; Q-3; R-2; S-1}$
$\text{P-2; Q-1; R-4; S-3}$
$\text{P-2; Q-3; R-4; S-1}$

GATE BT 2024 | Question: 36

Mon, 25 Mar 2024 19:16:15 +0000

Which of the following features help(s) in distinguishing alleles using restriction fragment length polymorphism (RFLP)?

Differences in the number of recognition sites for a given restriction enzyme
Differences in the ability of alleles to undergo recombination
Differences in the ability of alleles to undergo segregation
Differences in the number of tandem repeats

GATE BT 2023 | Question: 13

Sun, 21 May 2023 02:58:41 +0000

In hybridoma technology, which one of the following enzymes is absent in the myeloma cells that are used for monoclonal antibody production?

Hypoxanthine-guanine phosphoribosyltransferase
Alanine aminotransferase
Triose phosphate isomerase
Glycosyltransferase

GATE BT 2023 | Question: 14

Sun, 21 May 2023 02:58:40 +0000

Which of the following methods are used for detection of DNA and RNA, respectively?

Southern and Northern blotting
Southern and Western blotting
Northern and Southern blotting
Northern and Western blotting

GATE BT 2023 | Question: 34

Sun, 21 May 2023 02:58:25 +0000

Direct DNA transfer method(s) used for plant genetic engineering is(are)

microparticle bombardment
electroporation
polyethylene glycol treatment
Agrobacterium-mediated transformation

GATE BT 2022 | Question: 15

Sun, 20 Mar 2022 16:16:28 +0000

The recognition sequences of four Type-II restriction enzymes $\text{(RE)}$ are given below. The symbol $(\downarrow)$ indicates the cleavage site. Identify the $\text{RE}$ that generates sticky ends.

$\text{RE1} – 5’ \text{G}^{\downarrow} \text{GATCC} \; 3’$
$\text{RE2} – 5’ \text{CTG}^{\downarrow} \text{CAG} \; 3’$
$\text{RE3} – 5’ \text{CCC}^{\downarrow} \text{GGG} \; 3’$
$\text{RE4} – 5’ \text{AG}^{\downarrow} \text{CT} \; 3’$

GATE BT 2022 | Question: 16

Sun, 20 Mar 2022 16:16:28 +0000

Among individuals in a human population, minor variations exist in nucleotide sequences of chromosomes. These variations can lead to gain or loss of sites for specific restriction enzymes. Which of the following technique is used to identify such variations?

Polymerase dependent fragment insertion
Real-time polymerase chain reaction
Restriction fragment length polymorphism
Reverse transcriptase polymerase chain reaction

GATE BT 2021 | Question: 9

Mon, 01 Mar 2021 03:19:50 +0000

Which one of the following techniques/tools is $\text{NOT}$ used for inserting a foreign gene into a cell?

$\text{DNA}$ microarray
Electroporation
Gene gun
Microinjection

GATE BT 2021 | Question: 15

Mon, 01 Mar 2021 03:19:50 +0000

The enzyme which adds phosphate group to the free $5’$ terminus of a $\text{DNA}$ sequence is

adenosine kinase
alkaline phosphatase
polynucleotide kinase
terminal deoxynucleotidyl transferase

GATE2019: 27

Tue, 03 Nov 2020 12:03:23 +0000

For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated $DNA$?

$KpnI$
$DpnI$
$XhoI$
$MluI$

GATE2019: 31

Tue, 03 Nov 2020 12:03:23 +0000

Which of the following factors affect the fidelity of $DNA$ polymerase in polymerase chain reaction?

$Mg^{2+}$ concentration
$pH$
Annealing temperature

$P$ and $Q$ only
$P$ and $R$ only
$Q$ and $R$ only
$P, Q$ and $R$

GATE2020: 10

Tue, 03 Nov 2020 11:02:14 +0000

A vector derived from which one of the following viruses is used for high - frequency genomic integration of a transgene in animal cells?

Adenoviru
Adeno-associated virus
Lentivirus
Herpes simplex virus

GATE2020: 11

Tue, 03 Nov 2020 11:02:13 +0000

Which one of the following statements about $Agrobacterium \;Ti\; plasmid$ is $CORRECT$?

$Vir$ genes are located within the $T-DNA$ segment
Phytohormone biosynthesis genes are located outside the $T-DNA$ segment
Opine catabolism genes are located within the $T-DNA$ segment
Opine biosynthesis genes are located within the $T-DNA$ segment

GATE2019: 1

Tue, 03 Nov 2020 04:04:46 +0000

The Bt toxin gene from $Bacillus\; thuringiensis$ used to generate genetically modified crops is

$cry$
$cro$
$cdc$
$cre$

GATE2019: 8

Tue, 03 Nov 2020 04:04:45 +0000

Which one of the following techniques can be used to compare the expression of a large number of genes in two biological samples in a single experiment?

Polymerase chain reaction
$DNA$ microarray
Northern hybridization
Southern hybridization

GATE2016-6

Mon, 26 Mar 2018 16:59:53 +0000

Which one of the following is NOT a therapeutic agent based on nucleic acid for the treatment of genetic disorders?

Antisense oligonucleotide
Ribozyme
Aptamer
Avidin

GATE BT 2017 | Question: 20

Mon, 26 Mar 2018 16:43:19 +0000

A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg$^{2+}$. Which one of the following is missing in the reaction mixture?

Helicase
Single-stranded binding proteins
Primers
Reverse transciptase

GATE BT 2017 | Question: 44

Mon, 26 Mar 2018 16:43:16 +0000

Which one of the following CANNOT be a recognition sequence for a Type II restriction enzyme?

GAATTC
AGCT
GCGGCCGC
ATGCCT

GATE BT 2017 | Question: 51

Mon, 26 Mar 2018 16:43:15 +0000

EcoRI restriction sites on a $10$ kb DNA fragment are shown below.

Upon partial digestion, what are the lengths (in kb) of all the possible DNA fragments obtained?

$2,3,4,5,6,7,8$, and $10$
$2,3,4,5,6,$ and $7$
$2,3,4,$ and $7$
$2$ and $3$

GATE BT 2015 | Question: 30

Mon, 26 Mar 2018 15:10:47 +0000

In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be

$0$
$< 1$
$1$
$>1$

GATE BT 2015 | Question: 32

Mon, 26 Mar 2018 15:10:47 +0000

Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).

$$\textrm{5’ – GACCTGTGG- – – – – – – – – – – – – – – – – – ATACGGGAT – 3'}$$

$$\textrm{3’ – CTGGACACC – – – – – – – – – – – – – – – – – – TATGCCCTA – 5’}$$

$$\begin{array}{ll} & \textbf{Primers} \\ P. & \textrm{5’ – GACCTGTGG – 3’} \\ Q. & \textrm{5’ – CCACAGGTC – 3’} \\ R. & \textrm{5’ – TAGGGCATA – 3’} \\ S. & \textrm{5’ – ATCCCGTAT – 3’} \end{array}$$

$P$ and $R$
$P$ and $S$
$Q$ and $R$
$Q$ and $S$

GATE BT 2015 | Question: 65

Mon, 26 Mar 2018 15:10:42 +0000

A linear double stranded DNA of length $8$ kbp has three restriction sites. Each of these can either be a $\textit{Bam}\text{HI}$ or a $\textit{Hae}III$ site. The DNA was digested completely with both enzymes. The products were purified and subjected to an end-filling reaction using the Klenow fragment and $[\alpha-^{32}P]$-dCTP. The products of the end-filling reaction were purified, resolved by electrophoresis, stained with ethidium bromide (EtBr) and then subjected to autoradiography. The corresponding images are shown below

The numbers below each band in the sample lane in the autoradiograph represent their mean signal intensity in arbitrary units. Which one of the following options is the correct restriction map of the DNA?

GATE2014-34

Sun, 25 Mar 2018 23:27:37 +0000

The plasmid DNA was subjected to restriction digestion using the enzyme $\textit{EcoRI}$ and analysed on an agarose gel. Assuming digestion has worked (the enzyme was active), match the identity of the DNA bands shown in the image in $\text{Group I}$ with their identity in $\text{Group II}$.

$\begin{array}{|l|l|l|l|} \hline & \text{Group I} & & \text{Group II} \\ \hline P. & \text{Bands labeled as A} & 1. & \text{Nicked} \\ \hline Q. & \text{Bands labeled as B} & 2. & \text{Supercoiled} \\ \hline R. & \text{Bands labeled as C} & 3. & \text{Concatemers} \\ \hline S. & \text{Bands labeled as D} & 4. & \text{Linear} \\ \hline \end{array}$

$\text{P-3, Q-1, R-2, S-4}$
$\text{P-1, Q-4 R-3, S-2}$
$\text{P-4, Q-3, R-1, S-2}$
$\text{P-4, Q-1, R-2, S-3}$

GATE BT 2012 | Question: 13

Sun, 25 Mar 2018 08:38:43 +0000

Protein-DNA interactions $\text{in vivo}$ can be studied by

gel shift assay
Southern hybridization
chromatin immunoprecipitation assay
fluorescence $\text{in situ}$ hybridization assay

GATE BT 2012 | Question: 16

Sun, 25 Mar 2018 08:38:43 +0000

Which one of the following is NOT a protoplast fusion inducing agent?

Inactivated Sendai virus
$Ca^{2+}$ at alkaline pH
Polyethylene glycol
Colchicine