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Recent questions and answers in Analytical Techniques

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Q27 Match the entries in the Group I with the elution conditions in Group II.Group I Group IIP. Ion-exchange chromatography 1. Isocratic solventQ. Hydrophobic column chromatography 2. ArnpholytesR. Gel ltration chromatography 3. Increasing gradient of saltS. Chromatofocusing 4. Decreasing gradient of polarity P-4,Q-l,R-2,S-3 P-4,Q-3,R- 15-: P-3,Q-4,R-1,s-2 P-3,Q-4,R-2,S-1
asked Mar 26, 2018 in Analytical Techniques Milicevic3306 7.9k points
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The absorbance of a solution of tnptophan measured at 280 nm in a cuvette of 2.0 cm pathlength is 0.56 at pH 7. The molar extinction coefcient (a) for tiyptophan at 280 11111 is5600 Mlcnrl at pH .7. The concentration of tryptophan (in ilM) in the solution is
asked Feb 20, 2018 in Analytical Techniques gatecse 1.4k points
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\Vhich of the following technique(s) can be used to study conformational changes ininyoglobin'?P. Mass spectrometryQ. Fluorescence spectroscopyR. Circular dichroism spectroscopyS Light microscopy P only P and S only Q and R only S only
asked Feb 20, 2018 in Analytical Techniques gatecse 1.4k points
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A mixture contains three similarly sized peptides P. Q and R. The peptide P is positivelycharged. Q is weakly negative and R is strongly negative. If this mixture is passed throughan ion-exchange chromatography column containing an anionic resin. their order of elutionwill be P. Q. R R. Q. P Q. R. P P. Q and R elute together
asked Feb 20, 2018 in Analytical Techniques gatecse 1.4k points
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