# Recent questions in General Biotechnology

1
If the dissociation constant for solute-adsorbent binding is $K_D$, the retention time of the solute in a chromatography column increases with increasing $K_D$ decreases with increasing $K_D$ passes through minimum with increasing $K_D$ is independent of $K_D$
2
The most plausible explanation for a sudden increase of the respiratory quotient $(RQ)$ of a microbial culture is that cells are dying yield of biomass is increasing the fermentation rate is increasing relative to respiration rate the maintenance rate is decreasing
3
Gram-positive bacteria are generally resistant to complement-mediated lysis because thick peptidoglycan layer prevents insertion of membrane attack complex into the irmer membrane Gram-positive bacteria import the membrane attack complex and inactivate it membrane ... degraded by the proteases produced by the Gram-positive bacteria Gram-positive bacteria cannot activate the complement pathway
4
Which of the following essential element(s) is/are required as major supplement to enhance the bioremediation of oil spills by the resident bacteria? Sulfur Nitrogen and phosphorus Iron Carbon
5
The product(s) resulting from the hydrolysis of maltose is/are a mixture of $\alpha$-D-Glucose and $\beta$-D-Glucose a mixture of D-Glucose and L-Glucose $\alpha$-D-Glucose only $\beta$-D-Glucose only
6
Amino acid residue which is most likely to be found in the interior of water-soluble globular proteins is Threonine Aspartic acid Valine Histidine
7
Prior exposure of plants to pathogens is known to increase resistance to future pathogen attacks.This phenomenon is known as systemic acquired resistance hypersensitive response innate immunity antibody mediated response
8
Reactions between antibodies and antigens that are detected by precipitate formation in an agar gel are referred as immunoprecipitation assay immunodiffusion assay immunoaggregation assay immunofixation assay
9
The reactions leading to the formation of amino acids from the TCA cycle intermediates are carboxylation isomerization transamination decarboxylation
10
Which one of the receptors has the highest affinity for the ligand? $R1$ $R2$ $R3$ $R4$
11
Common Data for Questions 50 and 51:The binding eciency of three diamt receptorsRL R2 and R3 were tested against a ligand usirequilibrium dialysis, uith a constant concentration of receptor and varying concentrations of ligand- TlScatchard plot of receptor titration with diermt concentration of ... binding sites present on receptors R1 and R3, respectively are 1 and 4 l and l 4 and 1 2 and 2
12
The total number of fragments generated by the complete and sequential cleavage of the polypeptide given below by Trypsin followed by $CNBr$ is __________. $Phe-Trp-Met-Gly-Ala-Lys-Leu-Pro-Met-Asp-Gly-Arg-Cys-Ala-Gln$
13
Match the entries in the $\text{Group I}$ with the entries in $\text{Group II}$ ... $\text{P-2, Q-1, R-3, S-4}$ $\text{P-3, Q-4, R-2, S-1}$ $\text{P-1, Q-4, R-3, S-2}$
14
Match the entries in $\text{Group I}$ with the entries in $\text{Group II}$ ... $\text{P-4, Q-3, R-2, S-1}$ $\text{P-3, Q-2, R-1, S-4}$ $\text{P-2, Q-4, R-1, S-3}$
15
Match the herbicides in $\text{Group I}$ with the target enzymes in $\text{Group II}$ ... $\text{P-4, Q-1, R-2, S-3}$ $\text{P-4, Q-3, R-2, S-1}$ $\text{P-3, Q-2, R-4, S-1}$
16
Match the entries in the $\text{Group I}$ with the elution conditions in $\text{Group II}$ ... $\text{P-4, Q-3, R-1, S-2}$ $\text{P-3, Q-4, R-1, S-2}$ $\text{P-3, Q-4, R-2, S-1}$
17
Match the cell structures in $\text{Group I}$ with the organisms in $\text{Group II}$ ... $\text{P-4, Q-3, R-2, S-1}$ $\text{P-3, Q-4, R-1, S-2}$ $\text{P-4, Q-1, R-3, S-2}$
18
Match the antibiotics in $\text{Group I}$ wiith the targets in $\text{Group II}$ ... $\text{P-2, Q-4, R-3, S-1}$ $\text{P-4, Q-1, R-2, S-3}$ $\text{P-3, Q-4, R-2, S-1}$
19
In nature, $\text{Agrobacterium tumefaciens}$ mediated infection of plant cells leads to P. crown gall disease in plants Q. hairy root disease in plants R. transfer of $\text{T-DNA}$ into the plant chromosome S. transfer of Ri-plasmid into the plant cell S only P and R only Q and S only Q only
20
Match the entries in $\text{Group I}$ with the enzymes in $\text{Group II}$ ... $\text{P-4, Q-1, R-3, S-2}$ $\text{P-3, Q-1, R-4, S-2}$ $\text{P-3, Q-4, R-2, S-1}$
21
Match the commercial microbial sources in $\text{Group I}$ with the products in $\text{Group II}$ ... $\text{P-3,Q-1,R-4,S-2}$ $\text{P-1,Q-3,R-2,S-4}$ $\text{P-1,Q-3,R-4,S-2}$
22
Hypophosphatemia is manifested by an $X-$linked dominant allele.What proportion of the offsprings from a normal male and an affected heterozygous female will manifest the disease? $1/2$ sons and $1/2$daughters all daughters and no sons all sons and no daughters $1/4$ daughters and $1/4$ sons
23
The catalytic efficiency for an enzyme is defined as $K_{cat}$ $\frac{V_{max}}{K_{cat}}$ $\frac{k_{cat}}{K_m}$ $\frac{K_{cat}}{V_{max}}$
24
Which one of the following is an $ABC$ transporter? multidrug resistance protein acetylcholine receptor bacteriorhodopsin $ATP$ synthase
25
Endogenous antigens are presented on to the cell surface along with $MHC-II$ $MHC-I$ $F_c\gamma$receptor complement receptor
26
The role of an adjuvant isto prolong the persistence of antigen cross link the antigen increase the size of antigen avoid inflammation
27
Which one of the following aminoacids in proteins does $NOT$ undergo phosphorylation? Ser Thr Pro Tyr
28
The first humanized monoclonal antibody approved for the treatment of breast canceris Rituximab Cetuximab Bevacizumab Herceptin
29
Protein A, which has strong affinity to $F_C$ region of immunoglobulin, is extracted from $\text{Saccharomyces cerevisiae}$ $\text{Staphylococcus aureus}$ $\text{Streptococcus pyogenes}$ $\text{Streptococcus sanguis}$
30
Which one of the following modifications is common toboth protein and DNA? SUMOylation Nitrosylation Methylation Ubiquitination
31
Under alkaline conditions, DNA is more stable than RNA because RNA forms secondary structures RNA is a single stranded molecule RNA has uracil in place of thymidine RNA is susceptible to hydrolysis
32
A rod shaped bacterium has a length of 2 pm. diameter of 1 pm and density the same as thatof water. If proteins constitute 159-6 of the cell mass and the average protein has a mass of50 kDa. the number of proteins in the cell is(1 Da = 1.6 x 1034g)
33
Consider a simple uni-substrate enzyme that follows Michaelis-Menten kinetics. When theenzyme catalyzed reaction was carried out in the presence of 10 nM concentration of aninhibitor. there was no change in the maximal velocity. However. the slope of theLineweaver-Blu'k plot increased 3-fold. The dissociation constant for the enzyme-inhibitorcomplex (in nM) is
34
Consider the following alignment of two DNA sequences:AGTAACAA- -ACAssumino an afne 0a scono scheme of an identi matrix for substitution. a ga initiationE E 5' ..penalty of 1 and a gap extens1on penalty ot 0.1. the score of the alignment is (up to onedecimal place)
35
Yeast converts glucose to ethanol and carbon dioxide by glycolysis as per the followingreaction:C6H1206 > ZCJHsOH + 2C0:Assuming complete conversion. the amount of ethanol produced (in g) from 200 g of glucoseis (up to two decimal places)
36
IvIannualian cells in active growth phase were seeded at a density of 1>
37
In a chemostat. the feed ow rate and culture volume are 100 1111 h and 1.0 L. respectively.With glucose as substrate. the values of ppm and K; are 0.2 11'1 and 1 g L. respectively. For aglucose concentration of 10 g L in the feed. the efuent substrate concentration (in g L)is