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Recent questions in General Biotechnology

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1
If the dissociation constant for solute-adsorbent binding is $K_D$, the retention time of the solute in a chromatography column increases with increasing $K_D$ decreases with increasing $K_D$ passes through minimum with increasing $K_D$ is independent of $K_D$
asked Mar 26, 2018 in Biochemistry Milicevic3306 7.9k points
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2
The most plausible explanation for a sudden increase of the respiratory quotient $(RQ)$ of a microbial culture is that cells are dying yield of biomass is increasing the fermentation rate is increasing relative to respiration rate the maintenance rate is decreasing
asked Mar 26, 2018 in Microbiology Milicevic3306 7.9k points
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3
Gram-positive bacteria are generally resistant to complement-mediated lysis because thick peptidoglycan layer prevents insertion of membrane attack complex into the irmer membrane Gram-positive bacteria import the membrane attack complex and inactivate it membrane ... degraded by the proteases produced by the Gram-positive bacteria Gram-positive bacteria cannot activate the complement pathway
asked Mar 26, 2018 in Microbiology Milicevic3306 7.9k points
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4
Which of the following essential element(s) is/are required as major supplement to enhance the bioremediation of oil spills by the resident bacteria? Sulfur Nitrogen and phosphorus Iron Carbon
asked Mar 26, 2018 in Microbiology Milicevic3306 7.9k points
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5
The product(s) resulting from the hydrolysis of maltose is/are a mixture of $\alpha$-D-Glucose and $\beta$-D-Glucose a mixture of D-Glucose and L-Glucose $\alpha$-D-Glucose only $\beta$-D-Glucose only
asked Mar 26, 2018 in Biochemistry Milicevic3306 7.9k points
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6
Amino acid residue which is most likely to be found in the interior of water-soluble globular proteins is Threonine Aspartic acid Valine Histidine
asked Mar 26, 2018 in Biochemistry Milicevic3306 7.9k points
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7
Prior exposure of plants to pathogens is known to increase resistance to future pathogen attacks.This phenomenon is known as systemic acquired resistance hypersensitive response innate immunity antibody mediated response
asked Mar 26, 2018 in Immunology Milicevic3306 7.9k points
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8
Reactions between antibodies and antigens that are detected by precipitate formation in an agar gel are referred as immunoprecipitation assay immunodiffusion assay immunoaggregation assay immunofixation assay
asked Mar 26, 2018 in Immunology Milicevic3306 7.9k points
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9
The reactions leading to the formation of amino acids from the TCA cycle intermediates are carboxylation isomerization transamination decarboxylation
asked Mar 26, 2018 in Biochemistry Milicevic3306 7.9k points
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10
Which one of the receptors has the highest affinity for the ligand? $R1$ $R2$ $R3$ $R4$
asked Mar 26, 2018 in Immunology Milicevic3306 7.9k points
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11
Common Data for Questions 50 and 51:The binding eciency of three diamt receptorsRL R2 and R3 were tested against a ligand usirequilibrium dialysis, uith a constant concentration of receptor and varying concentrations of ligand- TlScatchard plot of receptor titration with diermt concentration of ... binding sites present on receptors R1 and R3, respectively are 1 and 4 l and l 4 and 1 2 and 2
asked Mar 26, 2018 in Immunology Milicevic3306 7.9k points
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12
The total number of fragments generated by the complete and sequential cleavage of the polypeptide given below by Trypsin followed by $CNBr$ is __________. $Phe-Trp-Met-Gly-Ala-Lys-Leu-Pro-Met-Asp-Gly-Arg-Cys-Ala-Gln$
asked Mar 26, 2018 in Biochemistry Milicevic3306 7.9k points
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13
Match the entries in the $\text{Group I}$ with the entries in $\text{Group II}$ ... $\text{P-2, Q-1, R-3, S-4}$ $\text{P-3, Q-4, R-2, S-1}$ $\text{P-1, Q-4, R-3, S-2}$
asked Mar 26, 2018 in Molecular Biology and Genetics: Milicevic3306 7.9k points
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14
Match the entries in $\text{Group I}$ with the entries in $\text{Group II}$ ... $\text{P-4, Q-3, R-2, S-1}$ $\text{P-3, Q-2, R-1, S-4}$ $\text{P-2, Q-4, R-1, S-3}$
asked Mar 26, 2018 in Molecular Biology and Genetics: Milicevic3306 7.9k points
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15
Match the herbicides in $\text{Group I}$ with the target enzymes in $\text{Group II}$ ... $\text{P-4, Q-1, R-2, S-3}$ $\text{P-4, Q-3, R-2, S-1}$ $\text{P-3, Q-2, R-4, S-1}$
asked Mar 26, 2018 in Biochemistry Milicevic3306 7.9k points
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16
Match the entries in the $\text{Group I}$ with the elution conditions in $\text{Group II}$ ... $\text{P-4, Q-3, R-1, S-2}$ $\text{P-3, Q-4, R-1, S-2}$ $\text{P-3, Q-4, R-2, S-1}$
asked Mar 26, 2018 in Analytical Techniques Milicevic3306 7.9k points
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17
Match the cell structures in $\text{Group I}$ with the organisms in $\text{Group II}$ ... $\text{P-4, Q-3, R-2, S-1}$ $\text{P-3, Q-4, R-1, S-2}$ $\text{P-4, Q-1, R-3, S-2}$
asked Mar 26, 2018 in Microbiology Milicevic3306 7.9k points
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18
Match the antibiotics in $\text{Group I}$ wiith the targets in $\text{Group II}$ ... $\text{P-2, Q-4, R-3, S-1}$ $\text{P-4, Q-1, R-2, S-3}$ $\text{P-3, Q-4, R-2, S-1}$
asked Mar 26, 2018 in Molecular Biology and Genetics: Milicevic3306 7.9k points
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19
In nature, $\text{Agrobacterium tumefaciens}$ mediated infection of plant cells leads to P. crown gall disease in plants Q. hairy root disease in plants R. transfer of $\text{T-DNA}$ into the plant chromosome S. transfer of Ri-plasmid into the plant cell S only P and R only Q and S only Q only
asked Mar 26, 2018 in Molecular Biology and Genetics: Milicevic3306 7.9k points
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20
Match the entries in $\text{Group I}$ with the enzymes in $\text{Group II}$ ... $\text{P-4, Q-1, R-3, S-2}$ $\text{P-3, Q-1, R-4, S-2}$ $\text{P-3, Q-4, R-2, S-1}$
asked Mar 26, 2018 in Biochemistry Milicevic3306 7.9k points
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21
Match the commercial microbial sources in $\text{Group I}$ with the products in $\text{Group II}$ ... $\text{P-3,Q-1,R-4,S-2}$ $\text{P-1,Q-3,R-2,S-4}$ $\text{P-1,Q-3,R-4,S-2}$
asked Mar 26, 2018 in Microbiology Milicevic3306 7.9k points
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22
Hypophosphatemia is manifested by an $X-$linked dominant allele.What proportion of the offsprings from a normal male and an affected heterozygous female will manifest the disease? $1/2$ sons and $1/2$daughters all daughters and no sons all sons and no daughters $1/4$ daughters and $1/4$ sons
asked Mar 26, 2018 in Molecular Biology and Genetics: Milicevic3306 7.9k points
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23
The catalytic efficiency for an enzyme is defined as $K_{cat}$ $\frac{V_{max}}{K_{cat}}$ $\frac{k_{cat}}{K_m}$ $\frac{K_{cat}}{V_{max}}$
asked Mar 26, 2018 in Biochemistry Milicevic3306 7.9k points
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24
Which one of the following is an $ABC$ transporter? multidrug resistance protein acetylcholine receptor bacteriorhodopsin $ATP$ synthase
asked Mar 26, 2018 in Biochemistry Milicevic3306 7.9k points
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25
Endogenous antigens are presented on to the cell surface along with $MHC-II$ $MHC-I$ $F_c\gamma$receptor complement receptor
asked Mar 26, 2018 in Molecular Biology and Genetics: Milicevic3306 7.9k points
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26
The role of an adjuvant isto prolong the persistence of antigen cross link the antigen increase the size of antigen avoid inflammation
asked Mar 26, 2018 in Immunology Milicevic3306 7.9k points
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27
Which one of the following aminoacids in proteins does $NOT$ undergo phosphorylation? Ser Thr Pro Tyr
asked Mar 26, 2018 in Biochemistry Milicevic3306 7.9k points
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28
The first humanized monoclonal antibody approved for the treatment of breast canceris Rituximab Cetuximab Bevacizumab Herceptin
asked Mar 26, 2018 in Immunology Milicevic3306 7.9k points
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29
Protein A, which has strong affinity to $F_C$ region of immunoglobulin, is extracted from $\text{Saccharomyces cerevisiae}$ $\text{Staphylococcus aureus}$ $\text{Streptococcus pyogenes}$ $\text{Streptococcus sanguis}$
asked Mar 26, 2018 in Immunology Milicevic3306 7.9k points
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30
Which one of the following modifications is common toboth protein and DNA? SUMOylation Nitrosylation Methylation Ubiquitination
asked Mar 26, 2018 in Biochemistry Milicevic3306 7.9k points
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31
Under alkaline conditions, DNA is more stable than RNA because RNA forms secondary structures RNA is a single stranded molecule RNA has uracil in place of thymidine RNA is susceptible to hydrolysis
asked Mar 26, 2018 in Molecular Biology and Genetics: Milicevic3306 7.9k points
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32
A rod shaped bacterium has a length of 2 pm. diameter of 1 pm and density the same as thatof water. If proteins constitute 159-6 of the cell mass and the average protein has a mass of50 kDa. the number of proteins in the cell is(1 Da = 1.6 x 1034g)
asked Feb 20, 2018 in Biochemistry gatecse 1.4k points
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33
Consider a simple uni-substrate enzyme that follows Michaelis-Menten kinetics. When theenzyme catalyzed reaction was carried out in the presence of 10 nM concentration of aninhibitor. there was no change in the maximal velocity. However. the slope of theLineweaver-Blu'k plot increased 3-fold. The dissociation constant for the enzyme-inhibitorcomplex (in nM) is
asked Feb 20, 2018 in Biochemistry gatecse 1.4k points
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1 answer
34
Consider the following alignment of two DNA sequences:AGTAACAA- -ACAssumino an afne 0a scono scheme of an identi matrix for substitution. a ga initiationE E 5' ..penalty of 1 and a gap extens1on penalty ot 0.1. the score of the alignment is (up to onedecimal place)
asked Feb 20, 2018 in Bioinformatics gatecse 1.4k points
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35
Yeast converts glucose to ethanol and carbon dioxide by glycolysis as per the followingreaction:C6H1206 > ZCJHsOH + 2C0:Assuming complete conversion. the amount of ethanol produced (in g) from 200 g of glucoseis (up to two decimal places)
asked Feb 20, 2018 in Biochemistry gatecse 1.4k points
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36
IvIannualian cells in active growth phase were seeded at a density of 1>
asked Feb 20, 2018 in Microbiology gatecse 1.4k points
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37
In a chemostat. the feed ow rate and culture volume are 100 1111 h and 1.0 L. respectively.With glucose as substrate. the values of ppm and K; are 0.2 11'1 and 1 g L. respectively. For aglucose concentration of 10 g L in the feed. the efuent substrate concentration (in g L)is
asked Feb 20, 2018 in Biochemistry gatecse 1.4k points
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38
5' capping of mRNA transcpts in eukaiyotes involves the following events:P. Addition of GMP on the 5' endQ. Removal of y-phosphate of the triphosphate on rst base at the 5' endR. 5'-5 linkage between GMP and the rst base at 5' endS. Addition of methyl group to N7 position of guanineWhich one of the following is the conect sequence of events? P. Q. R. S P. R. Q. S Q. P. R. S Q. P. S. R
asked Feb 20, 2018 in Molecular Biology and Genetics: gatecse 1.4k points
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39
Match the industrial products mentioned in Group I with their producer organisms inGroup IIGroup I Group IIP. Citric acid 1. T richodemza vindeQ. Cellulase 2. C losnidium acetobuo'lz'cmnR. Vitamin B12 3. Aspergilius nigerS Butanol 4. Propionibacrerium 'eudenreichii P- 4. Q- 3. R- 1. S-2 P-S. Q-l. R-Z. S-4 P-Z. Q- 1. R- 4 S3 P-3. Q-l. R-4. S-Z
asked Feb 20, 2018 in Microbiology gatecse 1.4k points
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40
A pedigree of an inheritable disease is shown below. O Unaffected Female @ Carrier Female . Affected Female|:l Unaffected Male 0 Carrier Male I Affected Male\Vhat type of inheritance does the disease follow? Autosomal dominant Xlinked dominant X-linked recessive Autosomal recessive
asked Feb 20, 2018 in Immunology gatecse 1.4k points
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