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Recent questions in Recombinant DNA Technology

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1
The 4-amino or 4-keto group of pyrimidine bases is located in the major groove of the double stranded DNA minor groove of the double stranded DNA minor groove of the B form DNA but not the A form DNA major groove of the B form DNA but not the A form DNA
asked Mar 26, 2018 in Recombinant DNA Technology Milicevic3306 7.9k points
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2
The $5$’ ends of the mature forms of the prokaryotic mRNAs and tRNAs are a triphosphate group in mRNAs and a monophosphate group in tRNAs triphosphate groups in both mRNAs and tRNAs monophosphate groups in both mRNAs and tRNAs a monophosphate group in mRNAs and a triphosphate group in tRNAs
asked Mar 26, 2018 in Recombinant DNA Technology Milicevic3306 7.9k points
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3
A DNA fragment of 5000bp needs to be isolated from $E.coli$ (genome size $4\times10^3 kb$) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are $16\times10^2$ $12\times10^2$ $8\times10^2$ $1.25\times10^2$
asked Mar 26, 2018 in Recombinant DNA Technology Milicevic3306 7.9k points
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4
A complete restriction digestion of a circular plasmid $(5000bp)$ was carried out with $HindIII,BamHIandEcoRIindividually.$ Restriction digestion yielded following fragments. $Plasmid + HindIII \rightarrow 1200bp\: and\: 3800bp$ $Plasmid + BamHI \rightarrow 5000bp$ $Plasmid + EcoRI \rightarrow 2500bp$ ... $EcoRI-1, BamHI- 1, HindIII-2$ $EcoRl-3, BamHI-2, HindIII-1$ $EcoRI-2, BamHI-2, HindIII-1$
asked Mar 26, 2018 in Recombinant DNA Technology Milicevic3306 7.9k points
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5
Of the two diploid species, species $I$ has $36$ chromosomes and species $II$ has $28$ chromosomes. How many chromosomes would be found in an allotriploid individual? $42$ or $54 $ $46$ or $50 $ $74$ or $86 $ $84$ or $108$
asked Mar 26, 2018 in Recombinant DNA Technology Milicevic3306 7.9k points
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6
The $RNA$ primer synthesized during the replication process in bacteria is removed by $DNA$ gyrase primase $DNA$ polymerase $I$ $DNA$ polymerase $II$
asked Mar 26, 2018 in Recombinant DNA Technology Milicevic3306 7.9k points
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7
The suitable substitution matrix to align closely related sequences is $\text{PAM 250 or BLOSSUM 80}$ $\text{PAM 40 or BLOSSUM 80}$ $\text{PAM 120 or BLOSSUM 40}$ $\text{PAM 250 or BLOSSUM 40}$
asked Mar 26, 2018 in Recombinant DNA Technology Milicevic3306 7.9k points
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8
Human genome sequencing project involved the construction of genomic library in bacterial artificial chromosome $pBR322$ bacteriophage $pcDNA3.1$
asked Mar 26, 2018 in Recombinant DNA Technology Milicevic3306 7.9k points
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9
The nucleotide analogue used in DNA sequencing by chain termination method is $l',3'-$dideoxy nucleoside triphosphate $2',3'-$dideoxy nucleoside triphosphate $2',4'-$dideoxy nucleoside triphosphate $2',5'-$dideoxy nucleoside triphosphate
asked Mar 26, 2018 in Recombinant DNA Technology Milicevic3306 7.9k points
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10
In nature, the horizontal gene transfer across bacteria is mediated by gene cloning followed by transformation conjugation and transformation conjugation only transformation only
asked Mar 26, 2018 in Recombinant DNA Technology Milicevic3306 7.9k points
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11
Phylum proteobacteria is subdivided into $\alpha-, \beta-, \gamma-, \delta-$ and $\epsilon-$ proteobacteria based on $G+C$ content $23S\; rRNA$ sequences $tRNA$ sequences $16S\; rRNA$ sequences
asked Mar 26, 2018 in Recombinant DNA Technology Milicevic3306 7.9k points
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12
The product of complete digestion of the plasmid shown below with EcoRI and HaeIII waspuried and used as a template in a reaction containing Klenow fragment of DNApolymerase. dNTPs and [a32P]-dATP in a suitable reaction buffer. The product thusobtained was puried and subjected to gel ... '1 kb 1Haelll t5' -GGCC-3HaeIII 3,_CCGG_5,5 kb TThe number of bands that will appear on the X-ray lm is
asked Feb 20, 2018 in Recombinant DNA Technology gatecse 1.4k points
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13
Match the classes of RNA molecules in Group I with their functions in Group II.Group I Group IIP. snoRNA l. Protects geimline from transposable elementsQ. piR-\'A 2. Blocks translation of selected mRNAR. miRNA 3. Template for telomere elongationS. snRNA 4. Modication and processing of rRNA5. Splicing of RNA transcripts P-3. Q-s. R-Z. 5-4 P-l. Q-3. R4, 5.5 P-l. Q-4. R-S. 5.2 P-4. Q-l. 12-3. 3-5
asked Feb 20, 2018 in Recombinant DNA Technology gatecse 1.4k points
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14
Genomic DNA isolated from a bacterium was digested with a restriction enzyme thatrecognizes a 6-base pair (bp) sequence. Assuming random distribution of bases. the averagelength (in bp) of the fragments generated is
asked Feb 20, 2018 in Recombinant DNA Technology gatecse 1.4k points
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15
\Vhich one of the following techniques is used to monitor RNA transcripts. both temporallyand spatially?(A) Northern blotting(B) In situ hybridization(C ) Southern blotting(D) Western blotting
asked Feb 20, 2018 in Recombinant DNA Technology gatecse 1.4k points
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