Most viewed questions in Recombinant DNA technology and Other Tools in Biotechnology

Most viewed questions in Recombinant DNA technology and Other Tools in Biotechnology

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1

GATE2018-54
The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and $[\alpha -^{32}P]$-dATP in a suitable reaction buffer. ... and subjected to gel electrophoresis followed by autoradiography. The number of bands that will appear on the X-ray film is _____________

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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2

GATE2018-28
Match the classes of RNA molecules in $\text{Group I}$ with their functions in $\text{Group II}$ ... $\text{P-1, Q-4, R-5, S-2}$ $\text{P-4, Q-1, R-2, S-5}$

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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3

GATE2018-17
Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially? Northern blotting In situ hybridization Southern blotting Western blotting

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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4

GATE2018-25
Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a $6$-base pair (bp) sequence. Assuming random distribution of bases, the average length (in bp) of the fragments generated is __________

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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5

GATE2014-19
The $5$’ ends of the mature forms of the prokaryotic mRNAs and tRNAs are a triphosphate group in mRNAs and a monophosphate group in tRNAs triphosphate groups in both mRNAs and tRNAs monophosphate groups in both mRNAs and tRNAs a monophosphate group in mRNAs and a triphosphate group in tRNAs

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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6

GATE2013-16
The suitable substitution matrix to align closely related sequences is $\text{PAM 250 or BLOSSUM 80}$ $\text{PAM 40 or BLOSSUM 80}$ $\text{PAM 120 or BLOSSUM 40}$ $\text{PAM 250 or BLOSSUM 40}$

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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7

GATE2014-16
The 4-amino or 4-keto group of pyrimidine bases is located in the major groove of the double stranded DNA minor groove of the double stranded DNA minor groove of the B form DNA but not the A form DNA major groove of the B form DNA but not the A form DNA

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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8

GATE2013-42
A complete restriction digestion of a circular plasmid $(5000bp)$ was carried out with $HindIII,BamHIandEcoRIindividually.$ Restriction digestion yielded following fragments. $Plasmid + HindIII \rightarrow 1200bp\: and\: 3800bp$ $Plasmid + BamHI \rightarrow 5000bp$ ... $EcoRl-3, BamHI-2, HindIII-1$ $EcoRI-2, BamHI-2, HindIII-1$

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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9

GATE2013-9
The nucleotide analogue used in DNA sequencing by chain termination method is $l',3'-$dideoxy nucleoside triphosphate $2',3'-$dideoxy nucleoside triphosphate $2',4'-$dideoxy nucleoside triphosphate $2',5'-$dideoxy nucleoside triphosphate

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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10

GATE2013-52
A DNA fragment of 5000bp needs to be isolated from $E.coli$ (genome size $4\times10^3 kb$) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are $16\times10^2$ $12\times10^2$ $8\times10^2$ $1.25\times10^2$

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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11

GATE2013-11
Phylum proteobacteria is subdivided into $\alpha-, \beta-, \gamma-, \delta-$ and $\epsilon-$ proteobacteria based on $G+C$ content $23S\; rRNA$ sequences $tRNA$ sequences $16S\; rRNA$ sequences

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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12

GATE2013-14
Of the two diploid species, species $I$ has $36$ chromosomes and species $II$ has $28$ chromosomes. How many chromosomes would be found in an allotriploid individual? $42$ or $54 $ $46$ or $50 $ $74$ or $86 $ $84$ or $108$

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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13

GATE2013-15
The $RNA$ primer synthesized during the replication process in bacteria is removed by $DNA$ gyrase primase $DNA$ polymerase $I$ $DNA$ polymerase $II$

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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14

GATE2013-8
Human genome sequencing project involved the construction of genomic library in bacterial artificial chromosome $pBR322$ bacteriophage $pcDNA3.1$

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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15

GATE2013-10
In nature, the horizontal gene transfer across bacteria is mediated by gene cloning followed by transformation conjugation and transformation conjugation only transformation only

Milicevic3306 asked in Recombinant DNA technology and Other Tools in Biotechnology Mar 26, 2018

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