# Recent questions and answers in Recombinant DNA Technology

1
The 4-amino or 4-keto group of pyrimidine bases is located in the major groove of the double stranded DNA minor groove of the double stranded DNA minor groove of the B form DNA but not the A form DNA major groove of the B form DNA but not the A form DNA
2
The $5$’ ends of the mature forms of the prokaryotic mRNAs and tRNAs are a triphosphate group in mRNAs and a monophosphate group in tRNAs triphosphate groups in both mRNAs and tRNAs monophosphate groups in both mRNAs and tRNAs a monophosphate group in mRNAs and a triphosphate group in tRNAs
3
A DNA fragment of 5000bp needs to be isolated from $E.coli$ (genome size $4\times10^3 kb$) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are $16\times10^2$ $12\times10^2$ $8\times10^2$ $1.25\times10^2$
4
A complete restriction digestion of a circular plasmid $(5000bp)$ was carried out with $HindIII,BamHIandEcoRIindividually.$ Restriction digestion yielded following fragments. $Plasmid + HindIII \rightarrow 1200bp\: and\: 3800bp$ $Plasmid + BamHI \rightarrow 5000bp$ $Plasmid + EcoRI \rightarrow 2500bp$ ... $EcoRI-1, BamHI- 1, HindIII-2$ $EcoRl-3, BamHI-2, HindIII-1$ $EcoRI-2, BamHI-2, HindIII-1$
5
Of the two diploid species, species $I$ has $36$ chromosomes and species $II$ has $28$ chromosomes. How many chromosomes would be found in an allotriploid individual? $42$ or $54$ $46$ or $50$ $74$ or $86$ $84$ or $108$
6
The $RNA$ primer synthesized during the replication process in bacteria is removed by $DNA$ gyrase primase $DNA$ polymerase $I$ $DNA$ polymerase $II$
7
The suitable substitution matrix to align closely related sequences is $\text{PAM 250 or BLOSSUM 80}$ $\text{PAM 40 or BLOSSUM 80}$ $\text{PAM 120 or BLOSSUM 40}$ $\text{PAM 250 or BLOSSUM 40}$
8
Human genome sequencing project involved the construction of genomic library in bacterial artificial chromosome $pBR322$ bacteriophage $pcDNA3.1$
9
The nucleotide analogue used in DNA sequencing by chain termination method is $l',3'-$dideoxy nucleoside triphosphate $2',3'-$dideoxy nucleoside triphosphate $2',4'-$dideoxy nucleoside triphosphate $2',5'-$dideoxy nucleoside triphosphate
10
In nature, the horizontal gene transfer across bacteria is mediated by gene cloning followed by transformation conjugation and transformation conjugation only transformation only
11
Phylum proteobacteria is subdivided into $\alpha-, \beta-, \gamma-, \delta-$ and $\epsilon-$ proteobacteria based on $G+C$ content $23S\; rRNA$ sequences $tRNA$ sequences $16S\; rRNA$ sequences
12
The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and $[\alpha -^{32}P]$-dATP in a suitable reaction buffer. The ... purified and subjected to gel electrophoresis followed by autoradiography. The number of bands that will appear on the X-ray film is _____________
13
Match the classes of RNA molecules in $\text{Group I}$ with their functions in $\text{Group II}$ ... $\text{P-1, Q-3, R-2, S-5}$ $\text{P-1, Q-4, R-5, S-2}$ $\text{P-4, Q-1, R-2, S-5}$
14
Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a $6$-base pair (bp) sequence. Assuming random distribution of bases, the average length (in bp) of the fragments generated is __________