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GATE2020: 27

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The $DNA$ sequence shown below is to be amplified by $PCR$:

$5{}'$ GCTAAGATCTGAATTTTCC$\dots\dots$TTGGGCAATAATGTAGCGC$3{}'$

$3{}'$ CGATTCTAGACTTAAAAGG$\dots\dots$AACCCGTTATTACATCGCG$3{}'$

Which one of the following pair of primers can be used for this amplification?

  1. $5{}'$ GGAAATTCAGATCTTAGT$3{}'$ and $5{}'$ TGGGCAATAATGTAGCGC$3{}'$
  2. $5{}'$ GCTAAGATCTGAATTTTC$3{}'$ and $5{}'$ GCGCTACATTATTGCCCA$3{}'$
  3. $5{}'$ CGGAAATTCAGATCTTAG$3{}'$ and $5{}'$ GCGCTACATTATTGCCCA$3{}'$
  4. $5{}'$ GCTAAGATCTGAATTTTC$3{}'$ and $5{}'$ TGGGCAATAATGTAGCGC$3{}'$
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