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441
GATE2015-21
Which one of the following is NOT a product of denitrification in Pseuadomonas? $N_2$ $N_2O$ $NO_2^-$ $NH_4^+$
Which one of the following is NOT a product of denitrification in Pseuadomonas?$N_2$$N_2O$$NO_2^-$$NH_4^+$
Milicevic3306
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Mar 26, 2018
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442
GATE2015-22
The determinant of the matrix $\begin{bmatrix} 3 & 0 & 0 \\ 2 & 5 & 0 \\ 6 & -8 & -4 \end{bmatrix}$ is __________
The determinant of the matrix $\begin{bmatrix} 3 & 0 & 0 \\ 2 & 5 & 0 \\ 6 & -8 & -4 \end{bmatrix}$ is __________
Milicevic3306
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gate2015
numerical-answers
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443
GATE2015-23
Which one of the following features is NOT required in a prokaryotic expression vector? $\textit{oriC}$ Selection marker CMV promoter Ribosome binding site
Which one of the following features is NOT required in a prokaryotic expression vector?$\textit{oriC}$Selection markerCMV promoterRibosome binding site
Milicevic3306
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gate2015
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444
GATE2015-24
Production of monoclonal antibodies by hybridoma technology requires splenocytes osteocytes hepatocytes thymocytes
Production of monoclonal antibodies by hybridoma technology requiressplenocytesosteocyteshepatocytesthymocytes
Milicevic3306
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445
GATE2015-25
Which one of the following is INCORRECT about a typical apoptotic cell? Phosphatidylserine is presented on the outer cell surface Cytochrome c is released from mitochondria Mitochondrial membrane potential does not change Annexin-V binds to the cell surface
Which one of the following is INCORRECT about a typical apoptotic cell?Phosphatidylserine is presented on the outer cell surfaceCytochrome c is released from mitochondria...
Milicevic3306
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Mar 26, 2018
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446
GATE2015-26
Identify the file format given below: $>$P$1$; JMFD Protein X – Homo sapiens MKALTARQQEVFDLIRDHISRTLRQQGDWL GDE FASTA NBRF GCG
Identify the file format given below:$>$P$1$; JMFDProtein X – Homo sapiensMKALTARQQEVFDLIRDHISRTLRQQGDWLGDEFASTANBRFGCG
Milicevic3306
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447
GATE2015-27
Which one of the following relations holds true for the specific growth rate $(\mu)$ of a microorganism in the death phase? $\mu=0$ $\mu<0$ $\mu=\mu_{\text{max}}$ $0<\mu<\mu_{\text{max}}$
Which one of the following relations holds true for the specific growth rate $(\mu)$ of a microorganism in the death phase?$\mu=0$$\mu<0$$\mu=\mu_{\text{max}}$$0<\mu<\mu_...
Milicevic3306
7.9k
points
Milicevic3306
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Mar 26, 2018
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448
GATE2015-28
How many $3$-tuples are possible for the following amino acid sequence? MADCMWDISEASE $4$ $5$ $11$ $12$
How many $3$-tuples are possible for the following amino acid sequence?MADCMWDISEASE$4$$5$$11$$12$
Milicevic3306
7.9k
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gate2015
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449
GATE2015-29
How many different protein sequences of $100$ residues can be generated using $20$ standard amino acids? $100^{20}$ $100 \times 20$ $20^{100}$ $100! \times 20!$
How many different protein sequences of $100$ residues can be generated using $20$ standard amino acids?$100^{20}$$100 \times 20$$20^{100}$$100! \times 20!$
Milicevic3306
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450
GATE2015-30
In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be $0$ $< 1$ $1$ $>1$
In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be$0$$< 1$$1$$>1$
Milicevic3306
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451
GATE2015-31
Which one of the following graphs represents uncompetitive inhibition?
Which one of the following graphs represents uncompetitive inhibition?
Milicevic3306
7.9k
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452
GATE2015-32
Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR). $\textrm{5' - GACCTGTGG- - - - - - - - - - - - - - - - - - ATACGGGAT - 3'}$ $\textrm{3' - CTGGACACC - - - - - - - - - - - - - - - - - - TATGCCCTA - 5'}$ ... $P$ and $R$ $P$ and $S$ $Q$ and $R$ $Q$ and $S$
Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).$$\textrm{5’ – GACCTGTGG- – – – – – – ...
Milicevic3306
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Mar 26, 2018
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453
GATE2015-35
The interaction between an antigen $(Ag)$ and a single-chain antibody $(Ab)$ was studied using Scatchard analysis. The result is shown below. The affinity of interaction and the total concentration of antibody, respectively, can be determined from slope and $Y$-intercept $Y$-intercept and slope $X$-intercept and slope slope and $X$-intercept
The interaction between an antigen $(Ag)$ and a single-chain antibody $(Ab)$ was studied using Scatchard analysis. The result is shown below.The affinity of interaction a...
Milicevic3306
7.9k
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Milicevic3306
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Mar 26, 2018
Others
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454
GATE2015-36
An isolated population on an island has the following genotypic frequencies: $\begin{array}{|c|c|c|c|} \hline \textrm{Genotype} & AA & Aa & aa \\ \hline \textrm{Frequency} & 0.3 & 0.4 & 0.3 \\ \hline \end{array}$ Assuming that there are only two alleles ($A$ and $a$) for the gene, the genotypic frequency of $AA$ in the next generation will be __________
An isolated population on an island has the following genotypic frequencies: $$\begin{array}{|c|c|c|c|} \hline \textrm{Genotype} & AA & Aa & aa \\ \hline \textrm{Frequenc...
Milicevic3306
7.9k
points
Milicevic3306
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Mar 26, 2018
Others
gate2015
numerical-answers
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0
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455
GATE2015-37
How many rooted and unrooted phylogenetic trees, respectively, are possible with four different sequences? $3$ and $15$ $15$ and $3$ $15$ and $12$ $12$ and $3$
How many rooted and unrooted phylogenetic trees, respectively, are possible with four different sequences?$3$ and $15$$15$ and $3$$15$ and $12$$12$ and $3$
Milicevic3306
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456
GATE2015-38
Match the compounds in $\text{Group I}$ with the correct entries in $\text{Group II}$ ... $\text{P-5, Q-2, R-1, S-3}$ $\text{P-4, Q-2, R-1, S-3}$ $\text{P-4, Q-5, R-3, S-1}$
Match the compounds in $\text{Group I}$ with the correct entries in $\text{Group II}$.$\begin{array}{|l|l|l|l|}\hline & \textbf{Group I} & & \textbf{Group II} \\ \hline ...
Milicevic3306
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457
GATE2015-41
Match the drugs in $\text{Group I}$ with their mechanism of action in $\text{Group II}$ ... $P-2, Q-6, R-3, S-5$ $P-1, Q-3, R-6, S-5$ $P-2, Q-3, R-6, S-4$
Match the drugs in $\text{Group I}$ with their mechanism of action in $\text{Group II}$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \textbf{Group II} \\ \hline ...
Milicevic3306
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458
GATE2015-43
Match the cells in $\text{Group I}$ with their corresponding entries in $\text{Group II}$ ... $\text{P-3, Q-1, R-2, S-5}$ $\text{P-3, Q-1, R-2, S-6}$
Match the cells in $\text{Group I}$ with their corresponding entries in $\text{Group II}$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \textbf{Group II} \\ \hlin...
Milicevic3306
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459
GATE2015-44
Oxygen transfer was measured in a stirred tank bioreactor using dynamic method. The dissolved oxygen tension was found to be $80 \%$ air saturation under steady state conditions. The measured oxygen tensions at $7$ s and $17$ s were $55\%$ and $68\%$ air saturation, respectively. The volumetric mass transfer coefficient $K_{La}$ is __________ $s^{-1}$
Oxygen transfer was measured in a stirred tank bioreactor using dynamic method. The dissolved oxygen tension was found to be $80 \%$ air saturation under steady state con...
Milicevic3306
7.9k
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Milicevic3306
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Mar 26, 2018
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gate2015
numerical-answers
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460
GATE2015-45
Match the microorganism in $\text{Group I}$ with their fermentation products in $\text{Group II}$ ... $\text{P-3, Q-4, R-1, S-2}$ $\text{P-3, Q-4, R-2, S-1}$
Match the microorganism in $\text{Group I}$ with their fermentation products in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \textbf{Group II}...
Milicevic3306
7.9k
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Mar 26, 2018
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461
GATE2015-46
Plasmid DNA $(0.5 \: \mu g)$ containing an ampicillin resistance marker was added to $200 \: \mu l$ of competent cells. The transformed competent cells were diluted $10,000$ times, out of which, $50 \: \mu l$ was plated on agar plates containing ampicillin. A total of $35$ colonies were obtained. The transformation efficiency is _______$\times 10^6 \: \text{ cfu} \cdot \mu g^{-1}$
Plasmid DNA $(0.5 \: \mu g)$ containing an ampicillin resistance marker was added to $200 \: \mu l$ of competent cells. The transformed competent cells were diluted $10,0...
Milicevic3306
7.9k
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Milicevic3306
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Mar 26, 2018
Others
gate2015
numerical-answers
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462
GATE2015-47
Match the reagents in $\text{Group I}$ with their preferred cleavage sites in $\text{Group II}$ ... $\text{P-1, Q-2, R-5, S-4}$ $\text{P-4, Q-2, R-5, S-3}$
Match the reagents in $\text{Group I}$ with their preferred cleavage sites in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \...
Milicevic3306
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Mar 26, 2018
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463
GATE2015-48
$\textit{Saccharomyces cerevisiae}$ produces ethanol by fermentation. The theoretical yield of ethanol from $2.5$ g of glucose is _________ g.
$\textit{Saccharomyces cerevisiae}$ produces ethanol by fermentation. The theoretical yield of ethanol from $2.5$ g of glucose is _________ g.
Milicevic3306
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Milicevic3306
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Mar 26, 2018
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gate2015
numerical-answers
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464
GATE2015-49
Choose the CORRECT sequence of steps involved in cytoplast production. Digestion of cell wall $\to$ protoplast viability $\to$ cybrid formation $\to$ osmotic stabilizer Osmotic stabilizer $\to$ digestion of cell wall $\to$ protoplast viability $\to$ cybrid ... wall $\to$ cybrid formation Osmotic stabilizer $\to$ digestion of cell wall $\to$ cybrid formation $\to$ protoplast viability
Choose the CORRECT sequence of steps involved in cytoplast production.Digestion of cell wall $\to$ protoplast viability $\to$ cybrid formation $\to$ osmotic stabilizerOsm...
Milicevic3306
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Milicevic3306
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Mar 26, 2018
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465
GATE2015-50
Match the antibiotics in $\text{Group I}$ with their modes of action in $\text{Group II}$ ... $\text{P-3, Q-2, R-1, S-4}$ $\text{P-1, Q-4, R-3, S-2}$
Match the antibiotics in $\text{Group I}$ with their modes of action in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \hline ...
Milicevic3306
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Mar 26, 2018
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466
GATE2015-51
The diameters of a large and a small vessel are $1.62$ m and $16.2$ cm. ,respectively. The vessels are geometrically similar and operated under similar volumetric agitated power input. The mixing time in the small vessel was found to be $15$ s. Determine the mixing tune (in seconds) in the large vessel. $15$ $30$ $61$ $122$
The diameters of a large and a small vessel are $1.62$ m and $16.2$ cm. ,respectively. The vessels are geometrically similar and operated under similar volumetric agitate...
Milicevic3306
7.9k
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Milicevic3306
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467
GATE2015-53
Consider the following multiple sequence alignment of four DNA sequences. $\begin{array}{cccc} A & C & T & A \\ A & C & T & G \\ A & G & T & C \\ A & G & C & T \end{array}$Shannon's entropy of the above alignment is ___________
Consider the following multiple sequence alignment of four DNA sequences.$$\begin{array}{cccc} A & C & T & A \\ A & C & T & G \\ A & G & T & C \\ A & G & C & T \end{arra...
Milicevic3306
7.9k
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Milicevic3306
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Mar 26, 2018
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gate2015
numerical-answers
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468
GATE2015-54
The $K_i$ of a novel competitive inhibitor designed against an enzyme is $2.5 \: \mu M$. The enzyme was assayed in the absence or presence of the inhibitor $(5 \: \mu M)$ under identical conditions. The $K_m$ in the presence of the inhibitor was found to be $30 \: \mu M$. The $K_m$ in the absence of the inhibitor is _________ $\mu M$
The $K_i$ of a novel competitive inhibitor designed against an enzyme is $2.5 \: \mu M$. The enzyme was assayed in the absence or presence of the inhibitor $(5 \: \mu M)$...
Milicevic3306
7.9k
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Milicevic3306
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Mar 26, 2018
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gate2015
numerical-answers
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469
GATE2015-55
A heterozygous tall plant $(Tt)$ was crossed with a homozygous dwarf plant $(tt)$. The resultant seeds were collected. If five seeds are chosen at random, then the probability (in $\%$) that exactly two of these seeds will yiIeld dwarf plants is __________
A heterozygous tall plant $(Tt)$ was crossed with a homozygous dwarf plant $(tt)$. The resultant seeds were collected. If five seeds are chosen at random, then the probab...
Milicevic3306
7.9k
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Milicevic3306
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Mar 26, 2018
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gate2015
numerical-answers
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470
GATE2015-56
Assuming random distribution of nucleotides, the average number of fragments generated upon digestion of a circular DNA of size $4.3 \times 10^5$ bp with $\textit{Alu}I (5 ' – AG \downarrow CT-3' )$ is _________$ \times 10^3$
Assuming random distribution of nucleotides, the average number of fragments generated upon digestion of a circular DNA of size $4.3 \times 10^5$ bp with $\textit{Alu}I (...
Milicevic3306
7.9k
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Milicevic3306
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Mar 26, 2018
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gate2015
numerical-answers
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471
GATE2015-57
A synchronous culture containing $1.8 \times 10^5$ monkey kidney cells was seeded into three identical flasks. The doubling time of these cells is $24$ h. After $24$ h, the cells from all the three flasks were pooled and dispensed equally into each well of three $6$-well plates. The number of cells in each well will be _________$\times 10^4$.
A synchronous culture containing $1.8 \times 10^5$ monkey kidney cells was seeded into three identical flasks. The doubling time of these cells is $24$ h. After $24$ h, t...
Milicevic3306
7.9k
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Milicevic3306
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Mar 26, 2018
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gate2015
numerical-answers
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472
GATE2015-58
An $\textit{in vitro}$ translation system can synthesize peptides m all three reading frames of the RNA template. When $\text{5' - UCUCUCUC --- (UC)_n---UCUCUCUC - 3'}$ was used as the template in this $\textit{in vitro}$ translation system, the synthesized ... peptides contained $33.3 \%$ each of serine. leucine, and proline. Deduce the codon for proline. $UCU$ $CUC$ $CCU$ $UCC$
An $\textit{in vitro}$ translation system can synthesize peptides m all three reading frames of the RNA template. When $\text{5’ - UCUCUCUC - (UC)_n -UCUCUCUC - 3’}$...
Milicevic3306
7.9k
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Milicevic3306
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Mar 26, 2018
Others
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473
GATE2015-59
Three distinct antigens $X, Y$ and $Z$ were used to raise antibodies. Antigen $Z$ was injected in a mouse on day zero followed by the administration of antigens $X$ and $Y$ on day $28$. A second injection of antigen $X$ was administered on day $70$. The antibody titers were ... will be high on day $84$ $X$-specific IgG will be high on day $42$ $Y$-specific IgG will be high on day $84$
Three distinct antigens $X, Y$ and $Z$ were used to raise antibodies. Antigen $Z$ was injected in a mouse on day zero followed by the administration of antigens $X$ and $...
Milicevic3306
7.9k
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Milicevic3306
asked
Mar 26, 2018
Others
gate2015
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0
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474
GATE2015-60
The standard free energy change $(\Delta G' ^\circ)$ for ATP hydrolysis is $-30 kJ \cdot \text{mole}^{-1}$. The $\textit{in vivo}$ concentrations of ATP, ADP and $P_i$ in $E \textit{.coli}$ are $7.90, 1.04$ and $7.90$ ... $(\Delta G)$ for ATP hydrolysis $\textit{in viva}$ is _______ $kJ \cdot \text{mole}^{-1}$.
The standard free energy change $(\Delta G' ^\circ)$ for ATP hydrolysis is $-30 kJ \cdot \text{mole}^{-1}$. The $\textit{in vivo}$ concentrations of ATP, ADP and $P_i$ in...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
numerical-answers
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475
GATE2015-61
In a fed-batch culture, $200 \text{ g . L}^{-1}$ glucose solution is added at a flow rate of $50 \text{ L . h}^{-1}$ . The initial culture volume (at quasi steady state) and the initial cell concentration are $600 \: L$ and $20 \text{g . L}^{-1}$ , respectively. The ... g substrate$^{-1}$. The cell concentration $g . L^{-1})$ at quasi steady state at $t=8 \:h$ is $40$ $52$ $60$ $68$
In a fed-batch culture, $200 \text{ g . L}^{-1}$ glucose solution is added at a flow rate of $50 \text{ L . h}^{-1}$ . The initial culture volume (at quasi steady state) ...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
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476
GATE2015-62
Cytoplasmic extract from the wild type strain of a bacterium has the ability to convert a colorless substrate $(S)$ to a colored product $(P)$ via three colorless intermediates $X, Y$ and $Z$ in that order. Each step of the pathway involves a specific ... No: no color developed) Based on the data, which one of the following is the correct order of enzymes involved in the pathway?
Cytoplasmic extract from the wild type strain of a bacterium has the ability to convert a colorless substrate $(S)$ to a colored product $(P)$ via three colorless interme...
Milicevic3306
7.9k
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Milicevic3306
asked
Mar 26, 2018
Others
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0
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477
GATE2015-63
Samples of bacterial culture taken at $5$ PM and then the next day at $5$ AM were found to have $10^4$ and $10^7$ cells.mL${-1}$, respectively. Assuming that both the samples were taken during the log phase of cell growth, the generation time of this bacterium will be ________$h$.
Samples of bacterial culture taken at $5$ PM and then the next day at $5$ AM were found to have $10^4$ and $10^7$ cells.mL${-1}$, respectively. Assuming that both the sam...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
numerical-answers
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0
answers
478
GATE2015-64
Biomass is being produced in a continuous stirred tank bioreactor of $750$ L capacity. The sterile feed containing $8 \: g \cdot L^{-1}$ glucose as substrate was fed at a flow rate of $150 \: L \cdot h^{-1}$ ... Determine the cell productivity $(g \cdot L^{-1} \cdot h^{-1})$ at steady state. $0.85$ $0.65$ $0.45$ $0.25$
Biomass is being produced in a continuous stirred tank bioreactor of $750$ L capacity. The sterile feed containing $8 \: g \cdot L^{-1}$ glucose as substrate was fed at a...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
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479
GATE2015-65
A linear double stranded DNA of length $8$ kbp has three restriction sites. Each of these can either be a $\textit{Bam}\text{HI}$ or a $\textit{Hae}III$ site. The DNA was digested completely with both enzymes. The products were ... autoradiograph represent their mean signal intensity in arbitrary units. Which one of the following options is the correct restriction map of the DNA?
A linear double stranded DNA of length $8$ kbp has three restriction sites. Each of these can either be a $\textit{Bam}\text{HI}$ or a $\textit{Hae}III$ site. The DNA was...
Milicevic3306
7.9k
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Milicevic3306
asked
Mar 26, 2018
Others
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480
GATE2014-6
The unit for specific substrate consumption rate in a growing culture is $\frac{g}{L-h}$ $\frac{g}{h}$ $\frac{g}{g-h}$ $\frac{gmoles}{L-h}$
The unit for specific substrate consumption rate in a growing culture is$\frac{g}{L-h}$$\frac{g}{h}$$\frac{g}{g-h}$$\frac{gmoles}{L-h}$
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Others
gate2014
numerical-estimation
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