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Shown below is a plasmid vector (P) and an insert (Q t. The insert was cloned into the BamI—II site
ofthe vector. The recombinant plasmid was isolated and digested with Bin/HI or 17:01. The results
from the digestion experiments are shown in (R).

is"
1‘.
x? S #6
samut {F Q°
O
5 kb plasmid BamHl Xhol 80mm 4 kb —
vector I 0.3 kb I I 3 kb _
l - 2 kb —
1 kb insert 1 kb _ _ _

from

P Q R

Which one of the following explains the digestion results shown in th.‘

(A) The insert did not ligate to the vector.

(B) One copy of the insert ligated to the vector.

(C’) The insert ligated to the Vector as two tandem copies

(D) The insert ligated to the vector as two copies but not in tandem

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