GATE2017-43

Question

Shown below id a plasmid vector (P) and an insert (Q). The insert was cloned into the BamHI site of the vector. The recombinant plasmid was isolated and digested with BamHi or XhoI. The results from the digestion experiments are shown in (R)

Which one of the following explains the digestion results shown in (R)

• A. The insert did not ligate to the vector.
• B. One copy of the insert ligated to the vector.
• C. The insert ligated to the Vector as two tandem copies
• D. The insert ligated to the vector as two copies but not in tandem