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Q44 The activity of lactate dehydrogenase can be measured by monitoring the follouing reaction:Pymvate + NADH -> Lactate + NADThe molar extinction coeicient of NADH at 340 nm is 6220 l\f.cml. NAD does not absorb atthis \\ ax elength. In an assay, 25 iiL of a sample of enzyme (containing 5 pg protein per m1.) asadded to a mixture of pV rm ate and N ADI-I to git e a total \ olume of 3 mL in 3 cm ette of 1 cmpathlength The rate of decrease in absorbance at 340 nm as 0.14 min .The specic actix it} ofthe enzyme ill be pmol min1 mg

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