Bio Technology
0 votes
image

Q44 The activity of lactate dehydrogenase can be measured by monitoring the follouing reaction:Pymvate + NADH -> Lactate + NADThe molar extinction coeicient of NADH at 340 nm is 6220 l\f.cml. NAD does not absorb atthis \\ ax elength. In an assay, 25 iiL of a sample of enzyme (containing 5 pg protein per m1.) asadded to a mixture of pV rm ate and N ADI-I to git e a total \ olume of 3 mL in 3 cm ette of 1 cmpathlength The rate of decrease in absorbance at 340 nm as 0.14 min .The specic actix it} ofthe enzyme ill be pmol min1 mg

in Others by (7.9k points)

Please log in or register to answer this question.

Welcome to GATE BioTechnology, where you can ask questions and receive answers from other members of the community.
455 questions
2 answers
0 comments
967 users