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soujanyareddy13 asked in Others Nov 3, 2020 edited Dec 13, 2020 by soujanyareddy13

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Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are $5.6$ and $6.8$. Which one of the following techniques can be used to separate them?

Denaturing polyacrylamide gel electrophoresis
Size-exclusion chromatography
Ion-exchange chromatography
Nickel affinity chromatography

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soujanyareddy13 asked in Others Nov 3, 2020 edited Dec 13, 2020 by soujanyareddy13

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