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GATE2019: 33

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The hexapeptide $P$ has an isoelectric point $(pI)$ of $6.9$. Hexapeptide $Q$ is a variant of $P$ that contains valine instead of glutamate at position $3$. The two peptides are analyzed by polyacrylamide gel electrophoresis at pH $8.0$. Which one of the following statements is CORRECT?

  1. $P$ will migrate faster than $Q$ towards the anode
  2. $P$ will migrate faster than $Q$ towards the cathode
  3. Both $P$ and $Q$ will migrate together
  4. $Q$ will migrate faster than $P$ towards the anode
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