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GATE2012-13
Milicevic3306
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Mar 25, 2018
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Oct 24, 2020
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Protein-DNA interactions $\text{in vivo}$ can be studied by
gel shift assay
Southern hybridization
chromatin immunoprecipitation assay
fluorescence $\text{in situ}$ hybridization assay
gate2012
Milicevic3306
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GATE2012-55
$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and a voidage ($\epsilon$) of $0.55$. The $K'_m$ and $V'_m$; for the immobilized enzyme are $0.72 \: gl^{-1}$ ... the above bioconversion will be $50 \: lh^{-1}$ $55 \: lh^{-1}$ $137 \: lh^{-1}$ $550 \: lh^{-1}$
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GATE2012-54
$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and a voidage ($\epsilon$) of $0.55$. The $K'_m$ and $V'_m$; for the immobilized enzyme are $0.72 \: gl^{-1}$ and ... time required for the steady state reactor operation will be $0.1 \: h$ $0.4 \: h$ $1.0 \: h$ $1.1 \: h$
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GATE2012-53
$\text{Statement for Linked Answer Questions 52 and 53:}$ An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding affinity (${K_d}$) of $10^{-8}$ ... operon is repressed and can only be induced with lactose repressed and cannot be induced with lactose not repressed expressed only when glucose and lactose are present
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GATE2012-52
$\text{Statement for Linked Answer Questions 52 and 53:}$ An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding affinity (${K_d}$) of $10^{-8}$ M and $10^{-9}$ M with and without lactose respectively, in the ... The molar concentration of the repressor in the cell 15 $\text{0.1 nM}$ $\text{1 nM}$ $\text{10 nM}$ $\text{100 nM}$
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GATE2012-51
Answer the question based on the information given below: The purification data for an enzyme is given below: ... $\text{P-3.4, Q-2.4, R-1.5, S-0.1}$ $\text{P-100, Q-71, R-44, S-29}$
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gate2012
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