# GATE2013-10

In nature, the horizontal gene transfer across bacteria is mediated by

1. gene cloning followed by transformation
2. conjugation and transformation
3. conjugation only
4. transformation only

edited

## Related questions

1
A DNA fragment of 5000bp needs to be isolated from $E.coli$ (genome size $4\times10^3 kb$) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are $16\times10^2$ $12\times10^2$ $8\times10^2$ $1.25\times10^2$
2
A complete restriction digestion of a circular plasmid $(5000bp)$ was carried out with $HindIII,BamHIandEcoRIindividually.$ Restriction digestion yielded following fragments. $Plasmid + HindIII \rightarrow 1200bp\: and\: 3800bp$ $Plasmid + BamHI \rightarrow 5000bp$ $Plasmid + EcoRI \rightarrow 2500bp$ ... $EcoRI-1, BamHI- 1, HindIII-2$ $EcoRl-3, BamHI-2, HindIII-1$ $EcoRI-2, BamHI-2, HindIII-1$
The suitable substitution matrix to align closely related sequences is $\text{PAM 250 or BLOSSUM 80}$ $\text{PAM 40 or BLOSSUM 80}$ $\text{PAM 120 or BLOSSUM 40}$ $\text{PAM 250 or BLOSSUM 40}$
The $RNA$ primer synthesized during the replication process in bacteria is removed by $DNA$ gyrase primase $DNA$ polymerase $I$ $DNA$ polymerase $II$
Of the two diploid species, species $I$ has $36$ chromosomes and species $II$ has $28$ chromosomes. How many chromosomes would be found in an allotriploid individual? $42$ or $54$ $46$ or $50$ $74$ or $86$ $84$ or $108$