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Recent questions and answers in Recombinant DNA technology and Other Tools in Biotechnology
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0
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1
GATE2014-16
The 4-amino or 4-keto group of pyrimidine bases is located in the major groove of the double stranded DNA minor groove of the double stranded DNA minor groove of the B form DNA but not the A form DNA major groove of the B form DNA but not the A form DNA
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gate2014
dna
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2
GATE2014-19
The $5$’ ends of the mature forms of the prokaryotic mRNAs and tRNAs are a triphosphate group in mRNAs and a monophosphate group in tRNAs triphosphate groups in both mRNAs and tRNAs monophosphate groups in both mRNAs and tRNAs a monophosphate group in mRNAs and a triphosphate group in tRNAs
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gate2014
cdna-and-dna
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3
GATE2013-52
A DNA fragment of 5000bp needs to be isolated from $E.coli$ (genome size $4\times10^3 kb$) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are $16\times10^2$ $12\times10^2$ $8\times10^2$ $1.25\times10^2$
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gate2013
ristriction-enzyme-plasmid
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4
GATE2013-42
A complete restriction digestion of a circular plasmid $(5000bp)$ was carried out with $HindIII,BamHIandEcoRIindividually.$ Restriction digestion yielded following fragments. $Plasmid + HindIII \rightarrow 1200bp\: and\: 3800bp$ $Plasmid + BamHI \rightarrow 5000bp$ ... $EcoRl-3, BamHI-2, HindIII-1$ $EcoRI-2, BamHI-2, HindIII-1$
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Recombinant DNA technology and Other Tools in Biotechnology
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gate2013
restriction
plasmid
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5
GATE2013-14
Of the two diploid species, species $I$ has $36$ chromosomes and species $II$ has $28$ chromosomes. How many chromosomes would be found in an allotriploid individual? $42$ or $54 $ $46$ or $50 $ $74$ or $86 $ $84$ or $108$
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Recombinant DNA technology and Other Tools in Biotechnology
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gate2013
chromosome
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6
GATE2013-15
The $RNA$ primer synthesized during the replication process in bacteria is removed by $DNA$ gyrase primase $DNA$ polymerase $I$ $DNA$ polymerase $II$
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gate2013
polymerase
0
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0
answers
7
GATE2013-16
The suitable substitution matrix to align closely related sequences is $\text{PAM 250 or BLOSSUM 80}$ $\text{PAM 40 or BLOSSUM 80}$ $\text{PAM 120 or BLOSSUM 40}$ $\text{PAM 250 or BLOSSUM 40}$
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gate2013
matrix
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0
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8
GATE2013-8
Human genome sequencing project involved the construction of genomic library in bacterial artificial chromosome $pBR322$ bacteriophage $pcDNA3.1$
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gate2013
human-dna
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0
answers
9
GATE2013-9
The nucleotide analogue used in DNA sequencing by chain termination method is $l',3'-$dideoxy nucleoside triphosphate $2',3'-$dideoxy nucleoside triphosphate $2',4'-$dideoxy nucleoside triphosphate $2',5'-$dideoxy nucleoside triphosphate
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Recombinant DNA technology and Other Tools in Biotechnology
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gate2013
dna-sequencing
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0
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10
GATE2013-10
In nature, the horizontal gene transfer across bacteria is mediated by gene cloning followed by transformation conjugation and transformation conjugation only transformation only
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gate2013
cloning
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0
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11
GATE2013-11
Phylum proteobacteria is subdivided into $\alpha-, \beta-, \gamma-, \delta-$ and $\epsilon-$ proteobacteria based on $G+C$ content $23S\; rRNA$ sequences $tRNA$ sequences $16S\; rRNA$ sequences
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Recombinant DNA technology and Other Tools in Biotechnology
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gate2013
dna-rna
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0
answers
12
GATE2018-54
The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and $[\alpha -^{32}P]$-dATP in a suitable reaction buffer. ... and subjected to gel electrophoresis followed by autoradiography. The number of bands that will appear on the X-ray film is _____________
gatecse
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Recombinant DNA technology and Other Tools in Biotechnology
Feb 20, 2018
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gatecse
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gate2018
recombinant
dna
technology
numerical-answers
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votes
0
answers
13
GATE2018-28
Match the classes of RNA molecules in $\text{Group I}$ with their functions in $\text{Group II}$ ... $\text{P-1, Q-4, R-5, S-2}$ $\text{P-4, Q-1, R-2, S-5}$
gatecse
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Recombinant DNA technology and Other Tools in Biotechnology
Feb 20, 2018
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gatecse
1.4k
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gate2018
recombinant
dna
technology
0
votes
0
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14
GATE2018-25
Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a $6$-base pair (bp) sequence. Assuming random distribution of bases, the average length (in bp) of the fragments generated is __________
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gate2018
recombinant
dna
technology
numerical-answers
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0
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15
GATE2018-17
Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially? Northern blotting In situ hybridization Southern blotting Western blotting
gatecse
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gate2018
recombinant
dna
technology
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Recent questions and answers in Recombinant DNA technology and Other Tools in Biotechnology