Recent questions tagged biochemistry

In nitrogen fixation, to reduce nitrogen to ammonia, which one of the following shows the correct order of electron flow?Ferredoxin → reductase → nitrogenaseNADH → reduct...

Which one of the following proteins would elute third in gel filtration chromatography from a mixture of four proteins given below?Insulin $(6 \text{ kDa})$Myoglobin $(17...

Which one of the following antibiotics blocks protein chain elongation by preventing the action of peptidyl transferase?BleomycinRifampicinChloramphenicolTetracycline

Match the carbohydrates in Column $\text{I}$ with the correct glycosidic linkages involved in Column $\text{II}$.\[\begin{array}{|l|l|}\hline\text{Column I} & \text{Colum...

Which one of the following options represents the products of light reactions of photosynthesis in plants?$\mathrm{O}_{2} / \mathrm{NADH} / \mathrm{H}_{2}$$\mathrm{O}_{2}...

Which of the following drugs inhibit(s) $\text{ATP-ADP}$ translocase?OligomycinAtractylosideAmytalBongkrekic acid

You are characterizing a new enzyme isolated and purified in the laboratory. If the maximum velocity of the enzyme is $1800 ~\mu$ moles $\mathrm{L}^{-1} \mathrm{~min}^{-1...

Given below is the Gibbs free energy change ( $\Delta \mathrm{G}$ ) in kilo Joules per electron equivalent ( $\mathrm{kJ} \mathrm{e}^{-} \mathrm{eq}^{-1}$ ) at pH $7.0$, ...

You have purified an enzyme using a series of chromatographic methods. It was observed that a $10 ~\mu \mathrm{g} \mathrm{~mL}^{-1}$ of this purified enzyme converted $10...

Match the genetic disorders in Column $\text{I}$ to the corresponding underlying cause in Column $\text{II}$\[\begin{array}{|l|l|}\hline\text{Column I} & \text{Column II}...

Match the biomolecules in Column $\text{I}$ with their function given in Column $\text{II}$\[\begin{array}{|l|l|}\hline\text{Column I} & \text{Column II} \\\hline\text{P....

By elemental analysis of a biomass sample, it was found that the cells in the given sample are composed of (by weight) $\mathrm{C}=50 \%, \mathrm{H}=8 \%, \mathrm{O}=20 \...

Which of the following is/are posttranslational modification(s) involved in epigenetic control of gene expression?Arginine methylationLysine acetylationCytosine methylati...

An enzyme-catalyzed reaction is found to have $\Delta \mathrm{G}=-100 \mathrm{~kJ} \mathrm{~mol}^{-1}$. Which of the following statements about this reaction is/are true?...

The electron equivalent per liter (e-eq $\mathrm{L}^{-1}$ ) of acetate ion solution of concentration $10 \mathrm{~g} \mathrm{~L}^{-1}$ is $\_\_\_\_$ $\mathrm{e}^{-} \math...

​​​​Which one of the following coenzymes is utilised by alanine racemase for the conversion of $L$-Alanine to $D$-Alanine?Pyridoxal phosphateThiamine pyrophosphateTetrahy...

​​​​​​Which of the following separation processes is/are based on molecular size?Size-exclusion chromatographyIon exchange chromatographyMembrane ultrafiltrationUltracent...

​​Which of the following show(s) optical activity at $100 \: \mathrm{mM}$ concentration in water?Solution of NaClSolution of D-GlucoseSolution of GlycineSolution of L-Pro...

​​​Which of the following compounds have the same degree of reduction per carbon-mole?GlucoseLactic acidAcetic acidFormic acid

For a mechanically reversible isobaric process taking place in a closed system involving $5$ moles of an ideal gas, the temperature increases from an initial value of $30...

Within the Michaelis-Menten framework, the ratio of $v_{0} / V_{\max }$ when $[\mathrm{S}]=20 \times K_{\mathrm{m}}$ is $\_\_\_\_\_$(Round off to two decimal places)

Correctly match the Inhibitor with its respective Function in mitochondrial respiration.\[\begin{array}{|l|}\hline\begin{array}{l|l}\textbf{Inhibitor} & \textbf{Function}...

​​​​​An octapeptide composed of these $L$-amino acids - Lys, Thr, Ser, Met, Arg, Trp, Tyr, Glu - was subjected to analyses with the following outcomes:$\text{P}$. The N-t...

​​​​Correctly match the Coenzyme with its respective involvement in a specific Reaction type.\begin{array}{|l|l|}\hline \textbf{Coenzyme } & \textbf{Reaction type} \\\hli...

​​​​​Which of the following statements is/are true regarding the effect of the concentration of metabolic intermediates on glycolysis in erythrocytes?Increased AMP levels...

​​​​​Which of the following amino acids contain(s) two chiral carbons?L-LeucineL-ThreonineL-IsoleucineL-Asparagine

In a fermentation process, each mole of glucose is converted to biomass $\left(\mathrm{CH}_{1.8} \mathrm{O}_{0.5} \mathrm{~N}_{0.2}\right)$, with a biomass yield coeffici...

The percentage of light that would pass through a sample with an absorbance of $2$ would be $\_\_\_\_\_$ $\%$ .(Round off to the nearest integer)

An NMR spectrometer operating at proton resonance frequency $(v)$ of $1 \: \mathrm{GHz}$ will have a magnetic field strength of $\_\_\_\_$ Tesla (T).The gyromagnetic rati...

For the coupled reactions given below\[\begin{array}{ll}\text { Glucose 6-phosphate }+\mathrm{H}_{2} \mathrm{O} \rightarrow \text { Glucose }+\mathrm{Pi} & \text { (React...

In adsorption chromatography, the adsorption of uncharged solute molecules onto a silica-based stationary phase is by $\_\_\_\_\_\_\_$.covalent bondselectrostatic interac...

$2$-mercaptoethanol breaks the $\_\_\_\_\_\_\_$ covalent bond between light and heavy chains of an immunoglobulin molecule.$\text{C-N}$ $\text{N-O}$$\text{S-C}$$\text{S-S...

An element that is present in a nucleotide but not in a nucleoside is $\_\_\_\_\_\_\_$carbonnitrogenoxygenphosphorous

Krebs $\text{(TCA)}$ cycle is $\_\_\_\_\_\_$ pathway.only an anaboliconly a catabolic an amphibolica pyogenic

If a denatured protein of human origin is injected into a rabbit, antibodies generated will recognize the $\_\_\_\_\_\_$ structure of the protein.primarysecondarytertiary...