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Recent questions and answers in Biochemistry

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GATE2014-7
If the dissociation constant for solute-adsorbent binding is $K_D$, the retention time of the solute in a chromatography column increases with increasing $K_D$ decreases with increasing $K_D$ passes through minimum with increasing $K_D$ is independent of $K_D$

Milicevic3306 asked in Biochemistry Mar 26, 2018

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2

GATE2014-17
The product(s) resulting from the hydrolysis of maltose is/are a mixture of $\alpha$-D-Glucose and $\beta$-D-Glucose a mixture of D-Glucose and L-Glucose $\alpha$-D-Glucose only $\beta$-D-Glucose only

Milicevic3306 asked in Biochemistry Mar 26, 2018

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3

GATE2014-18
Amino acid residue which is most likely to be found in the interior of water-soluble globular proteins is Threonine Aspartic acid Valine Histidine

Milicevic3306 asked in Biochemistry Mar 26, 2018

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4

GATE2014-24
The reactions leading to the formation of amino acids from the TCA cycle intermediates are carboxylation isomerization transamination decarboxylation

Milicevic3306 asked in Biochemistry Mar 26, 2018

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5

GATE2013-43
The total number of fragments generated by the complete and sequential cleavage of the polypeptide given below by Trypsin followed by $CNBr$ is __________. $Phe-Trp-Met-Gly-Ala-Lys-Leu-Pro-Met-Asp-Gly-Arg-Cys-Ala-Gln$

Milicevic3306 asked in Biochemistry Mar 26, 2018

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6

GATE2013-33
Match the herbicides in $\text{Group I}$ with the target enzymes in $\text{Group II}$ ... $\text{P-4, Q-3, R-2, S-1}$ $\text{P-3, Q-2, R-4, S-1}$

Milicevic3306 asked in Biochemistry Mar 26, 2018

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7

GATE2013-32
Match the entries in $\text{Group I}$ with the enzymes in $\text{Group II}$ ... $\text{P-4, Q-1, R-3, S-2}$ $\text{P-3, Q-1, R-4, S-2}$ $\text{P-3, Q-4, R-2, S-1}$

Milicevic3306 asked in Biochemistry Mar 26, 2018

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8

GATE2013-13
The catalytic efficiency for an enzyme is defined as $K_{cat}$ $\frac{V_{max}}{K_{cat}}$ $\frac{k_{cat}}{K_m}$ $\frac{K_{cat}}{V_{max}}$

Milicevic3306 asked in Biochemistry Mar 26, 2018

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9

GATE2013-12
Which one of the following is an $ABC$ transporter? multidrug resistance protein acetylcholine receptor bacteriorhodopsin $ATP$ synthase

Milicevic3306 asked in Biochemistry Mar 26, 2018

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10

GATE2013-5
Which one of the following aminoacids in proteins does $NOT$ undergo phosphorylation? Ser Thr Pro Tyr

Milicevic3306 asked in Biochemistry Mar 26, 2018

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11

GATE2013-2
Which one of the following modifications is common toboth protein and DNA? SUMOylation Nitrosylation Methylation Ubiquitination

Milicevic3306 asked in Biochemistry Mar 26, 2018

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12

GATE2018-55
A rod shaped bacterium has a length of $2 \: \mu m$, diameter of $1 \mu m$ and density the same as that of water. If proteins constitute $15 \%$ of the cell mass and the average protein has a mass of $50$ kDa, the number of proteins in the cell is __________ ($1$ Da $= 1.6 \times 10^{-24}$g)

gatecse asked in Biochemistry Feb 20, 2018

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13

GATE2018-53
Consider a simple uni-substrate enzyme that follows Michaelis-Menten kinetics. When the enzyme catalyzed reaction was carried out in the presence of $10$ nM concentration of an inhibitor, there was no change in the maximal velocity. However. the slope of the Lineweaver-Burk plot increased $3$-fold. The dissociation constant for the enzyme-inhibitor complex (in nM) is ____________

gatecse asked in Biochemistry Feb 20, 2018

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14

GATE2018-52
First order deactivation rate constants for soluble and immobilized amyloglucosidase enzyme are $0.03 \text{ min}^{-1}$ and $0.005 \text{ min}^{-1}$, respectively. The ratio of half-life of the immobilized enzyme to that of the soluble enzyme is (rounded off to the nearest integer) ___________

gatecse asked in Biochemistry Feb 20, 2018

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15

GATE2018-49
Yeast converts glucose to ethanol and carbon dioxide by glycolysis as per the following reaction: $C_6H_{12}O_6 \to 2C_2H_5OH + 2CO_2$Assuming complete conversion, the amount of ethanol produced (in $g$) from $200 \: g$ of glucose is (up to two decimal places) ____________

gatecse asked in Biochemistry Feb 20, 2018

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16

GATE2018-47
In a chemostat, the feed flow rate and culture volume are $100$ ml/h and $1.0$ L, respectively. With glucose as substrate, the values of $\mu_{\text{max}}$ and $K_s$ are $0.2 \: h^{-1}$ and $1 \: g/L$, respectively. For a glucose concentration of $10 \: g/L$ in the feed, the effluent substrate concentration (in $g/L$) is _______

gatecse asked in Biochemistry Feb 20, 2018

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17

GATE2018-35
Which of the following are true with regard to anaerobic respiration in bacteria? The final electron acceptor is an inorganic substance other than molecular oxygen The number of ATP molecules produced per glucose molecule is more than that produced in aerobic respiration The number of ATP ... phosphorylation is used to generate ATP P and S only Q and S only P and R only P, Q and S only

gatecse asked in Biochemistry Feb 20, 2018

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18

GATE2018-6
Which of the following components constitute a molecular mechanics force field? Bond stretching Bond angle bending Torsional bond rotation Non-bonded interactions P and Q only P, Q and R only P, Q and S only P, Q, R and S

gatecse asked in Biochemistry Feb 20, 2018

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