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Recent questions and answers in Bioprocess Engineering and Process Biotechnology

0 votes
4 answers
1
In a batch culture of Penicillium chrysogenum, the maximum penicillin synthesis occurs during the lag phase exponential phase stationary phase death phase
answered Feb 16 in Bioprocess Engineering and Process Biotechnology Seema MP 140 points
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1 answer
2
A fed batch culture was operated with intermittent addition of glucose solution at a flow rate of $\text{200 ml h}^{-1}$. The values of $K_s,\: \mu_m$ and $D$ are $\text{0.3 g l}^{-1}, 0.4\:h^{-1}$ and $\text{0.1 h}^{-1}$, respectively. Determine the concentration of growth limiting substrate $(gl^{-1})$ in the reactor at quasi-steady state. ______________
answered Jan 1 in Bioprocess Engineering and Process Biotechnology Rama Karn 140 points
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1 answer
3
At the end of a batch culture, glucose solution is added at a flow rate of $200 \: ml/h$. If the culture volume after $2$ h of glucose addition is $1000$ ml, the initial culture volume (in ml) is _________
answered Aug 2, 2020 in Bioprocess Engineering and Process Biotechnology shalu_0202 140 points
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0 answers
4
Which of the following is employed for the repeated use of enzymes in bioprocesses? polymerization immobilization ligation isomerization
asked Mar 26, 2018 in Bioprocess Engineering and Process Biotechnology Milicevic3306 7.9k points
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0 answers
5
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6
A solution was prepared by dissolving $\text{100 mg}$ of protein $X$ in $\text{100 ml}$ of water. Molecular weight of protein $X$ is $\text{15,000 Da};$ Avogadro’s number = $6.022\times 10^{23}$. Calculate the molarity$(\mu M)$ of the resulting solution. $66.6$ $6.6$ $0.67$ $0.067$
asked Mar 26, 2018 in Bioprocess Engineering and Process Biotechnology Milicevic3306 7.9k points
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0 answers
7
The maximum cell concentration $(\text{g l}^{-1})$ expected in a bioreactor with initial cell concentration of $\text{1.75 g l}^{-1}$ and an initial glucose concentration of $\text{125 g l}^{-1}$ is ($Y_{X/S} = 0.6\:g\:cell/g$ substrate) ____________.
asked Mar 26, 2018 in Bioprocess Engineering and Process Biotechnology Milicevic3306 7.9k points
0 votes
0 answers
8
A batch bioreactor is to be scaled up from $\text{10 to 10,000}$ liters. The diameter of the large bioreactor is $10$ times that of the small bioreactor. The agitator speed in the small bioreactor is $\text{450 rpm}$. Determine the agitator speed (rpm) of the large bioreactor with same impeller tip speed as that of the small bioreactor. ____________
asked Mar 26, 2018 in Bioprocess Engineering and Process Biotechnology Milicevic3306 7.9k points
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0 answers
9
A chemostat is operated at a dilution rate of $0.6\; h^{-1}$. At steady state, the biomass concentration in the exit stream was found to be $30\; gl^{-1}$The biomass productivity $(g\; l^{-1}h^{-1})$ after $3h$ of steady state operation will be ___________.
asked Mar 26, 2018 in Bioprocess Engineering and Process Biotechnology Milicevic3306 7.9k points
0 votes
0 answers
10
Moist heat sterilization of spores at $121 ^\circ C$ follows first order kinetics as per the expression: $\dfrac{dN}{dt}=-k_dN$ where, $N$ is the number of viable spores, $t$I is the time, $k_d$ is the rate constant and $\dfrac{dN}{dt}$ is the rate of change ... reduce the number of viable spores from an initial value of $10^{10}$ to a final value of $1$ is (up to two decimal places) __________
asked Feb 20, 2018 in Bioprocess Engineering and Process Biotechnology gatecse 1.4k points
0 votes
0 answers
11
A microbial strain is cultured in a $100$ L stirred fermenter for secondary metabolite production. If the specific rate of oxygen uptake is $0.4 \: h^{-1}$ and the oxygen solubility in the broth is $8 $ mg/L, then the volumetric mass transfer coefficient $(K_La)$ (in $s^{-1}$) of oxygen required to achieve a maximum cell concentration of $12$ g/L is (up to two decimal places) ___________
asked Feb 20, 2018 in Bioprocess Engineering and Process Biotechnology gatecse 1.4k points
0 votes
0 answers
12
An aqueous solution containing $6.8$ mg/L of an antibiotic is extracted with amyl acetate. If the partition coefficient of the antibiotic is $170$ and the ratio of water to solvent is $85$, then the extraction factor is ________
asked Feb 20, 2018 in Bioprocess Engineering and Process Biotechnology gatecse 1.4k points
0 votes
0 answers
13
Shear stress versus shear rate behavior of four different types of fluids (I, II, III and IV) are shown in the figure below. Which one of the following options is correct? I-Newtonian, II-Bingham plastic, III-Dilatant, IV-Pseudoplastic I-Pseudoplastic ... -Bingham plastic I-Newtonian, II-Pseudoplastic, III-Bingham plastic, IV-Dilatant I-Newtonian, II-Bingham plastic, III-Pseudoplastic, IV-Dilatant
asked Feb 20, 2018 in Bioprocess Engineering and Process Biotechnology gatecse 1.4k points
0 votes
0 answers
14
Which one of the following bioreactor configurations is the basis for a trickling biological filter? Stirred tank Packed bed Air lift Fluidized bed
asked Feb 20, 2018 in Bioprocess Engineering and Process Biotechnology gatecse 1.4k points
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