Recent questions tagged microbiology

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In nitrogen fixation, to reduce nitrogen to ammonia, which one of the following shows the correct order of electron flow?Ferredoxin → reductase → nitrogenaseNADH → reduct...
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Which one of the following antibiotics blocks protein chain elongation by preventing the action of peptidyl transferase?BleomycinRifampicinChloramphenicolTetracycline
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The RNA primer synthesized during bacterial DNA replication is removed by $\_\_\_\_$.DNA gyraseDNA Polymerase $\text{I}$PrimaseDNA Polymerase $\text{III}$
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The correct sequence of the four steps involved in anaerobic production of biogas from complex organics is:Hydrolysis → Acidogenesis → Acetogenesis → MethanogenesisMethan...
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To determine the viable cell count of a bacterial culture, you have plated $50 ~\mu \mathrm{~L}$ of a $100$ -fold diluted sample of the culture on a nutrient agar plate a...
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A microbial culture is being grown in a bioreactor with continuous aeration. The dissolved oxygen probe provided with the system shows a reading of zero. In this context,...
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A batch sterilizer is being operated at $121^{\circ} \mathrm{C}$ for sterilizing a medium containing microbial cells. Assume that the thermal deactivation of cells is a f...
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A microbe that follows Monod growth kinetics on a limiting substrate (maximum specific growth rate of $0.5 \mathrm{~h}^{-1}$ and Monod constant of $0.1 ~\mathrm{mg} \math...
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It is desired to cultivate $150$ mg (dry weight) microbial cells with an empirical formula of $\mathrm{C}_{5} \mathrm{H}_{7} \mathrm{O}_{2} \mathrm{~N}$. If the phosphoru...
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​​​​​Koch's postulate was established by Robert Koch while working on a disease caused byMycobacterium tuberculosisBacillus anthracisStreptococcus pneumoniaeBacillus subt...
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​​​​​Corynebacterium diphtheriae causes diphtheria in humans, only when this bacterium is infected byphage $\beta$epsilon phage$T4$ phagelambda phage
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​​​​Which of the following features is/are used to distinguish Archaea from Bacteria?Gram-stainingPeptidoglycan in the cell wallPresence of N -acetylglucosamine$16 S$ rRN...
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If the doubling time of a bacterial population is $3$ hours, then its average specific growth rate during this period is $\_\_\_\_\_$ $\mathrm{h}^{-1}$.(Round off to two ...
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​​​Correctly match the Microorganisms with their respective Nutrition and energy requirement.\[\begin{array}{|l|}\hline\begin{array}{l|l}\textbf{Microorganisms} & \textbf...
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A $2 \: \mathrm{L}$ bioreactor is being operated as a chemostat, at a flow rate of $0.8 \: \mathrm{~L} / \mathrm{h}$ and sterile feed of $10 \: \mathrm{~g} / \mathrm{L}$ ...
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The relationship that involves the exchange of nutrients between two different species for their mutual growth is called $\_\_\_\_\_\_\_\_$antagonismcommensalismparasitis...
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Which one of the following bacteriophages has a genome composed of single stranded circular DNA?$\emptyset \text{X} 174$$\lambda$$\text{T}5$$\text{P}1$
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If the rate at which E. coli divides is $0.5 \mathrm{~h}^{-1}$, then its doubling time is $\_\_\_\_\_\_\_$ $\text{h}$.
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The decimal reduction time of a microbe during sterilization at $120{ }^{\circ} \mathrm{C}$ with a first order thermal death rate constant of $1 \mathrm{~min}^{-1}$ will ...
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Match the disease (Column I) with its biological vector (Column II).\begin{array}{|c|c|c|}\hline \text{Column I} & \text{Column II} \\\hline P. \text{Chagas disease} & ...
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Which of the following factors can affect the growth of a microbial culture in a batch cultivation process?$\mathrm{pH}$ of the medium Osmolarity of the mediumSubstrate c...
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Under complete cell washout condition in a chemostat with sterile feed, which of the following statements is/are correct?Biomass concentration in the reactor is maximumS...
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Aspergillus niger is grown in a $10,000$ L stirred batch bioreactor under aerated conditions to produce citric acid. At steady state oxygen transfer conditions, the speci...
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E. coli is inoculated in a shake flask containing nutrient rich medium. The initial number of viable cells in the medium is $10^2$. After few hours, the number of viable ...
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Which one of the following statements is TRUE about leghemoglobin?It binds oxygen to protect nitrogenaseIt binds hemoglobin to protect oxygenaseIt binds oxygen to protect...
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The correct sequence of events during bacteriophage infection of a bacterial cell islanding $\rightarrow$ attachment $\rightarrow$ tail contraction $\rightarrow$ penetrat...
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Match the type of bacteria in Group I with their respective growth properties in Group II.Group IGroup IIP. Halophile1. Grows optimally between $20^{\circ} \mathrm{C}$ an...
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Match the virus in Group I with the type of genome it contains in Group II.Group IGroup IIP. T4 bacteriophage1. dsRNAQ. SARS-CoV-22. ssDNAR. Pseudomonas phage $\phi 6$3. ...
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Which of the following statement(s) is(are) TRUE about fluoroquinolone drugs?They contain quinolone ring(s)They inhibit RNA polymeraseThey bind to bacterial topoisomerase...
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E. coli is cultivated in a chemostat operated at a dilution rate of $0.2 h^{-1}$. The values of biomass yield due to oxygen consumption and the steady state biomass conce...
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E. coli cultivated at $298 \mathrm{~K}$ uptakes an uncharged compound (A) by passive diffusion. The intracellular and extracellular concentrations of $\mathrm{A}$ are $0...
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The following schematic diagram shows a chemostat with cell recyclewhere $\mathrm{F}_0$ and $\mathrm{F}_{\mathrm{r}}$ are the volumetric flow rates (in $L \cdot h^{-1}$ )...
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Match the media component (Column I) with its role (Column II).$$\begin{array} {ll} \textbf{Column I} & \textbf{Column I} \\ \text{P. Sucrose} & \text{1. Anti-foam agen...
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In binomial nomenclature, the name of a bacterial strain is written with the first letter of __________ word(s) being capitalized.firstsecondneitherfirst and second
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The type of nucleic acid present in $\lambda$-phage isDouble stranded $\text{DNA}$Single stranded circular $\text{DNA}$Single stranded $\text{DNA}$Single stranded $\text{...
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Match the microbial product (Column I) with its respective application (Column II).$$\begin{array} {lll} \textbf{Column I} & & \textbf{Column II} \\ \text{P. Methane} & &...
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A microorganism is grown in a batch culture using glucose as a carbon source. The apparent growth yield is $0.5 \frac{\text{g biomass}}{\text{g substrate}}.$ The initial ...
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Which of the following is(are) $\text{COMMON}$ feature(s) for both aerobic and anaerobic bacterial cultures?Glycolysis$\text{NAD}^{+}$ is the oxidising agentOxidative pho...
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Which of the following statement(s) is(are) CORRECT regarding the $lac$ operon in $E. coli$ when grown in the presence of glucose and lactose?At low glucose level, the op...
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Emerging viruses such as $\text{SARS-Co V2}$ cause epidemics. Which of the following process(es) contribute to the rise of such viruses?Mutation of existing virusJumping ...