Recent questions tagged dna

The RNA primer synthesized during bacterial DNA replication is removed by $\_\_\_\_$.DNA gyraseDNA Polymerase $\text{I}$PrimaseDNA Polymerase $\text{III}$

The contour length of a B-DNA molecule that encodes a bacterial protein of $33 \: \text{kDa}$ is $\_\_\_\_$ $\mathrm{ nm}$.Consider the average molecular weight of an ami...

​​​​Which of the following statements about initiation of DNA replication in eukaryotes is/are true?DNA replication is initiated at the origin of replication licensed by ...

Which of the following methods are used for detection of DNA and RNA, respectively?Southern and Northern blotting Southern and Western blottingNorthern and Southern blott...

Which of the following vector(s) is(are) used to clone a DNA fragment of size $220 \mathrm{~kb}$?Bacterial artificial chromosomeYeast artificial chromosomeCosmids$\text{p...

DNA sample collected from an unidentified bacterial species $(Y)$ contains $13 \%$ of adenine. The $G+C$ content (in percentage) of $Y$ is ____________.

If $1000 \mathrm{bp}$ of a double-helical DNA weighs $1 \times 10^{-18} \mathrm{gm}$ and distance between two $\mathrm{bp}$ is $0.34 \mathrm{~nm}$, the total amount of DN...

The type of nucleic acid present in $\lambda$-phage isDouble stranded $\text{DNA}$Single stranded circular $\text{DNA}$Single stranded $\text{DNA}$Single stranded $\text{...

Adenine can undergo a spontaneous change to hypoxanthine in a cell, leading to a $\text{DNA}$ base pair mismatch. The $\text{CORRECT}$ combination of enzymes that are inv...

The enzyme which adds phosphate group to the free $5’$ terminus of a $\text{DNA}$ sequence isadenosine kinasealkaline phosphatasepolynucleotide kinaseterminal deoxynucleo...

A $\text{DNA}$ solution of $50 \: \mu g \: mL^{-1}$ concentration gives an absorbance of $1.0$ at $260 \textit{ nm}$. An aliquot of $20 \: \mu L$ from a $50 \: \mu L$ pur...

The possible number of $\textit{Sal}\text{I}$ restriction sites in a $9\text{ kb}$ double-stranded $\text{DNA}$, with all four bases occurring in equal proportion (rounde...

For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated $DNA$?$KpnI$$DpnI$$XhoI$$MluI$

The $DNA$ sequence shown below is to be amplified by $PCR$:$5{}'$ GCTAAGATCTGAATTTTCC$\dots\dots$TTGGGCAATAATGTAGCGC$3{}'$$3{}'$ CGATTCTAGACTTAAAAGG$\dots\dots$AACCCGTTAT...

Determine the correctness or otherwise of the following Assertion [$a$] and the Reason [$r$].Assertion [$a$]: Dam methylase protects $E$. coli $DNA$ from phage endonuclea...

The sequence of a $1$ $Mb$ long $DNA$ is random. This $DNA$ has all four bases occurring in equal proportion. The number of nucleotides, on average, between two successiv...

$E$ coli was grown in $^{15}N$ medium for several generations. Cells were then transferred to $^{14}N$ medium, allowed to grow for $4$ generations and $DNA$ was isolated ...

The amino acid sequence of a peptide is $Phe-Leu-lle-Met-Ser-Leu$. The number of codons that encode the amino acids present in this peptide is given below:Phe: $2$ codons...

Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, $\rho$ of a double stranded $Cs^+DNA$ is given ...

A DNA sequence, $\textrm{5’ -ATGGACGTGCTTCCCAAAGCATCGGGC- 3’}$, is mutated to obtain$\textrm{5'-ATGGACGTGCTTC}\textbf{a}\textrm{CAAAGCATCGGGC- 3'}$$\textrm{5'-ATGGACGTGCT...

A $1.2$ kb DNA fragment was cloned into $\textit{Bam}$HI and $\textit{Eco}$RI sites located on a $2.8$ kb cloning vector. The $\textit{Bam}$HI and $\textit{Eco}$RI sites ...

The transcription factor $X$ binds a $10$ base pair DNA stretch. In the DNA of an organism, $X$ was found to bind at $20$ distinct sites. An analysis of these $20$ bindin...

EcoRI restriction sites on a $10$ kb DNA fragment are shown below.Upon partial digestion, what are the lengths (in kb) of all the possible DNA fragments obtained?$2,3,4,5...

A DNA strand of length $25$ nm wraps diametrically around the circumference of a spherical histone-octamer once. The radius (nm) of the histone-octamer is _______Given da...

In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be$0$$< 1$$1$$>1$

Consider the following multiple sequence alignment of four DNA sequences.$$\begin{array}{cccc} A & C & T & A \\ A & C & T & G \\ A & G & T & C \\ A & G & C & T \end{arra...

Assuming random distribution of nucleotides, the average number of fragments generated upon digestion of a circular DNA of size $4.3 \times 10^5$ bp with $\textit{Alu}I (...

The 4-amino or 4-keto group of pyrimidine bases is located in themajor groove of the double stranded DNAminor groove of the double stranded DNAminor groove of the B form ...

Topological Winding number of a $\text{2.0 kb}$ covalently closed circular DNA was found to be $191$ with a writhing number of $-4$. Hence, its $\text{LINKING NUMBER}$ an...

The length of the minimum unique stretch of DNA sequence that can be found only once in a $3$ billion base pairs long genome is$14$$15$$16$$18$

Which of the following statements with respect to the orientation of the nitrogenous bases to the pentose sugars, and the puckering of the sugar, is correct?Anti, and 2’-...

A DNA fragment of $5000bp$ needs to be isolated from E.coli (genome size $4\times10^3kb$) genomic library.The number of clones to represent this fragment in genomic libra...

A single base pair of DNA weighs $1.1 \times 10^{-21}$ grams. How many picomoles of a plasmid vector of length $2750$ bp are contained in 1 $\mu$g of purified DNA?$0.30$$...

Which one of the following DNA sequences carries an invert repeat?ATGAGCCCCGAGTATACTCGGGGCTCATATGAGCCGGCTCTATACTCGGCCGAGATATGAGCCGAGCCTAACTCGGCTCGGATATGAGCCTATGGTATACTCGG...

Protein-DNA interactions $\text{in vivo}$ can be studied bygel shift assaySouthern hybridizationchromatin immunoprecipitation assayfluorescence $\text{in situ}$ hybridiza...

In mismatch correction repair, the parental DNA strand is distinguished from the daughter strand byacetylationphosphorylationmethylationglycosylation

The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA pol...

Match the classes of RNA molecules in $\text{Group I}$ with their functions in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ ...

Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a $6$-base pair (bp) sequence. Assuming random distribution of bases, the ave...