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The product of complete digestion of the plasmid shown below with EcoRI and HaeIII waspuried and used as a template in a reaction containing Klenow fragment of DNApolymerase. dNTPs and [a32P]-dATP in a suitable reaction buffer. The product thusobtained was puried and subjected to gel electrophoresis followed by autoradiography.3 kbHaelll t560' Rom 5' -GAATTC-33' -CTTAAG-5'1 kb 1Haelll t5' -GGCC-3HaeIII 3,_CCGG_5,5 kb TThe number of bands that will appear on the X-ray lm is

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