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Moist heat sterilization of spores at 121 C follows rst order kinetics as per the expression:where. N is the number of viable spores. I is the time. kd is the rate constant and(IN . . - . is the rate 01 change of viable spores.(itIf kd value is 1.0 minl. the time (in minutes.) required to reduce the number of viable sporesfrom an initial value of 1010 to a nal value of 1 is (up to two decimal places)

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