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Recent questions tagged recombinant

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GATE2018-54
The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and $[\alpha -^{32}P]$-dATP in a suitable reaction buffer. ... and subjected to gel electrophoresis followed by autoradiography. The number of bands that will appear on the X-ray film is _____________

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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GATE2018-28
Match the classes of RNA molecules in $\text{Group I}$ with their functions in $\text{Group II}$ ... $\text{P-1, Q-4, R-5, S-2}$ $\text{P-4, Q-1, R-2, S-5}$

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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GATE2018-25
Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a $6$-base pair (bp) sequence. Assuming random distribution of bases, the average length (in bp) of the fragments generated is __________

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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GATE2018-17
Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially? Northern blotting In situ hybridization Southern blotting Western blotting

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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