Recent questions tagged recombinant

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GATE2018-54
The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and $[\alpha -^{32}P]$-dATP in a suitable reaction buffer. ... and subjected to gel electrophoresis followed by autoradiography. The number of bands that will appear on the X-ray film is _____________

The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and $[\alpha -^{32}P]$-dATP in a suitable reaction buffer. The ... purified and subjected to gel electrophoresis followed by autoradiography. The number of bands that will appear on the X-ray film is _____________

asked Feb 20, 2018 in Recombinant DNA Technology gatecse 1.4k points

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GATE2018-28
Match the classes of RNA molecules in $\text{Group I}$ with their functions in $\text{Group II}$ ... $\text{P-1, Q-4, R-5, S-2}$ $\text{P-4, Q-1, R-2, S-5}$

Match the classes of RNA molecules in $\text{Group I}$ with their functions in $\text{Group II}$ ... $\text{P-1, Q-3, R-2, S-5}$ $\text{P-1, Q-4, R-5, S-2}$ $\text{P-4, Q-1, R-2, S-5}$

asked Feb 20, 2018 in Recombinant DNA Technology gatecse 1.4k points

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GATE2018-25
Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a $6$-base pair (bp) sequence. Assuming random distribution of bases, the average length (in bp) of the fragments generated is __________

Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a $6$-base pair (bp) sequence. Assuming random distribution of bases, the average length (in bp) of the fragments generated is __________

asked Feb 20, 2018 in Recombinant DNA Technology gatecse 1.4k points

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GATE2018-17
Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially? Northern blotting In situ hybridization Southern blotting Western blotting

Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially? Northern blotting In situ hybridization Southern blotting Western blotting

asked Feb 20, 2018 in Recombinant DNA Technology gatecse 1.4k points

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Recent questions tagged recombinant