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GATE2018-54
The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and $[\alpha -^{32}P]$-dATP in a suitable reaction buffer. ... and subjected to gel electrophoresis followed by autoradiography. The number of bands that will appear on the X-ray film is _____________

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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GATE2018-45
An aqueous solution containing $6.8$ mg/L of an antibiotic is extracted with amyl acetate. If the partition coefficient of the antibiotic is $170$ and the ratio of water to solvent is $85$, then the extraction factor is ________

gatecse asked in Bioprocess Engineering and Process Biotechnology Feb 20, 2018

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GATE2018-44
Moist heat sterilization of spores at $121 ^\circ C$ follows first order kinetics as per the expression: $\dfrac{dN}{dt}=-k_dN$ where, $N$ is the number of viable spores, $t$I is the time, $k_d$ is the rate constant and $\dfrac{dN}{dt}$ is the rate of ... the number of viable spores from an initial value of $10^{10}$ to a final value of $1$ is (up to two decimal places) __________

gatecse asked in Bioprocess Engineering and Process Biotechnology Feb 20, 2018

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GATE2018-28
Match the classes of RNA molecules in $\text{Group I}$ with their functions in $\text{Group II}$ ... $\text{P-1, Q-4, R-5, S-2}$ $\text{P-4, Q-1, R-2, S-5}$

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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GATE2018-26
In leguminous plants, both the rhizobium genes and the plant genes influence nodulation and nitrogen fixation. Which one of the following functions is NOT encoded by the host plant genes? Production of inducers that modify rhizobial cell wall Production of flavonoid inducers Establishment of contact between bacteria and legume Root hair curling

gatecse asked in Plant, Animal and Microbial Biotechnology Feb 20, 2018

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GATE2018-25
Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a $6$-base pair (bp) sequence. Assuming random distribution of bases, the average length (in bp) of the fragments generated is __________

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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GATE2018-17
Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially? Northern blotting In situ hybridization Southern blotting Western blotting

gatecse asked in Recombinant DNA technology and Other Tools in Biotechnology Feb 20, 2018

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