# Recent questions tagged technology

1
The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and $[\alpha -^{32}P]$-dATP in a suitable reaction buffer. The ... purified and subjected to gel electrophoresis followed by autoradiography. The number of bands that will appear on the X-ray film is _____________
2
Moist heat sterilization of spores at $121 ^\circ C$ follows first order kinetics as per the expression: $\dfrac{dN}{dt}=-k_dN$ where, $N$ is the number of viable spores, $t$I is the time, $k_d$ is the rate constant and $\dfrac{dN}{dt}$ is the rate of change ... reduce the number of viable spores from an initial value of $10^{10}$ to a final value of $1$ is (up to two decimal places) __________
3
An aqueous solution containing $6.8$ mg/L of an antibiotic is extracted with amyl acetate. If the partition coefficient of the antibiotic is $170$ and the ratio of water to solvent is $85$, then the extraction factor is ________
4
Match the classes of RNA molecules in $\text{Group I}$ with their functions in $\text{Group II}$ ... $\text{P-1, Q-3, R-2, S-5}$ $\text{P-1, Q-4, R-5, S-2}$ $\text{P-4, Q-1, R-2, S-5}$
Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a $6$-base pair (bp) sequence. Assuming random distribution of bases, the average length (in bp) of the fragments generated is __________