Recent questions tagged protein

Which one of the following proteins would elute third in gel filtration chromatography from a mixture of four proteins given below?Insulin $(6 \text{ kDa})$Myoglobin $(17...

Which one of the following antibiotics blocks protein chain elongation by preventing the action of peptidyl transferase?BleomycinRifampicinChloramphenicolTetracycline

BLAST and its extensions can be used to determine similarity of a query protein sequence with which of the following database(s)?A database of translated nucleotide seque...

Messenger $\text{RNAs (mRNAs)}$ translate to generate polypeptides and the translation terminates at the $\text{UGA, UAG}$ or $\text{UAA}$ stop codons. The $\text{AGA}$ c...

​​​​A recombinant protein is secreted extracellularly in soluble form by an $E. coli$ culture. Which of the following downstream processes is/are involved in the purifica...

The contour length of a B-DNA molecule that encodes a bacterial protein of $33 \: \text{kDa}$ is $\_\_\_\_$ $\mathrm{ nm}$.Consider the average molecular weight of an ami...

​​​​​An octapeptide composed of these $L$-amino acids - Lys, Thr, Ser, Met, Arg, Trp, Tyr, Glu - was subjected to analyses with the following outcomes:$\text{P}$. The N-t...

​​​​Which of the following proteins is/are involved in intraflagellar transport?MicrotubulesMyosinActinKinesin

If a denatured protein of human origin is injected into a rabbit, antibodies generated will recognize the $\_\_\_\_\_\_$ structure of the protein.primarysecondarytertiary...

Which one of the following programs is used for finding distantly related (or remote) protein homologs?BLASTNBLASTXPSI-BLASTTBLASTX

Which one of the following is used for global alignment of two protein sequences?Chou-Fasman methodGarnier-Osguthorpe-Robson (GOR) methodNeedleman-Wunsch algorithmSmith-W...

If there are three unrooted trees for four protein sequences, the number of rooted trees for the same number of sequences is _______________.

The number of different possible ways of forming five intramolecular disulfide bonds with ten cysteine residues of a protein is ______________.

A protein has three identical sites arranged at the vertices of an equilateral triangle. If one site is filled with a dye (donor), the measured quantum yield $\left(\phi_...

Which of the following are CORRECT about protein structure?Secondary structure is formed by a repeating pattern of interactions among the polypeptide backbone atomsTertia...

A protein without its prosthetic group is known asapoproteinhemoproteinholoproteinlipoprotein

Tertiary structure of a protein consisting of $\alpha$-helices and $\beta$-strands can be determined bycircular dichroism spectroscopymass spectrometrynuclear magnetic re...

In general, which one of the following statements is $NOT \;CORRECT$?Hydrogen bonds result from electrostatic interactionsHydrogen bonds contribute to the folding energy ...

Which of the following statements are $CORRECT$ when a protein sequence database is searched using the $BLAST$ algorithm?A larger E-value indicates higher sequence simila...

Which one of the following amino acid residues will destabilize an $\alpha$-helix when inserted in the middle of the helix?ProVallIeLeu

The molecular mass of a protein is $22\:kDa$. The size of the $\text{cDNA}$ (excluding the untranslated regions) that codes for this protein is __________ $kb$ (rounded o...

Protein $A$ and protein $B$ form a covalent complex. Gel filtration chromatography of this complex showed a peak corresponding to $200 \:kDa$. $SDS-PAGE$ analysis of this...

An algorithm was designed to find globins in protein sequence databases. A database which has $78$ globin sequences was searched using this algorithm. The algorithm retri...

Protein $P$ becomes functional upon phosphorylation of a serine residue. Replacing this serine with _____________ will result in a phosphomimic mutant of $P$.alanineaspar...

Amino acid sequences of cytochrome $c$ and ribulose $5$-phosphate epimerase from $40$ organisms were chosen and phylogenetic trees were obtained for each of these two pro...

Which one of the following is a database of protein sequence motifs?$PROSITE$$TrEMBL$$SWISSPROT$$PDB$

Which set of the following events occurs during the elongation step of translation?Attachment of mRNA with the smaller subunit of ribosomeLoading of correct aminoacyl-tRN...

The molar extinction coeffcients of Trp and Tyr at $280$ nm are $5690$ and $1280 \: M^{-1} \cdot cm^{-1}$, respectively. The polypeptide chain of yeast alcohol dehydrogen...

Analysis of a hexapeptide using enzymatic cleavage reveals the following result:Amino acid composition of the peptide is: $2R, A, V, S, Y$Trypsin digestion yields two fra...

Which one of the following techniques can be used to determine the structure of a $15$ kDa globular protein at atomic resolution?Raman spectroscopyIR spectroscopyUV spect...

If a protein contains four cysteine residues, the number of different ways they can simultaneously form two intra-molecular disulphide bonds is __________.

During protein synthesis, tRNAs are NOT involved incharginginitiationelongationtermination

Which one of the following graphs represents the kinetics of protein precipitation by addition of ammonium sulphate? On the $Y$-axis, [Protein] represents the concentrati...

Match the proteins in $\textbf{Group I}$ with cellular processes in $\textbf{Group II}$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} & & \text{Group II} \\ \hline P...

The circular dichroism spectra of three proteins $P$, $Q$, and $R$ are given below:Choose the correct combination.$P: \alpha- \text{helix}, Q: \beta- \text{sheet}, \text{...

Levinthal’s paradox is related toprotein secretionprotein degradationprotein foldingprotein trafficking

Which one of the following amino acids has the highest probability to be found on the surface of a typical globular protein in aqueous environment?AlaValArgIle

How many different protein sequences of $100$ residues can be generated using $20$ standard amino acids?$100^{20}$$100 \times 20$$20^{100}$$100! \times 20!$