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Analysis of a hexapeptide using enzymatic cleavage reveals the following result:

  • Amino acid composition of the peptide is: $2R, A, V, S, Y$
  • Trypsin digestion yields two fragments and the compositions are: $(R A,V)$ and $(R, S, Y)$
  • Chymotrypsin digestion yields two fragments and the compositions are: $(A, R, V, Y)$ and $(R, S)$
  • Digestion with carboxypeptidase A yields no cleavage product.

Given: Trypsin cleaves at carboxyl side of $R$.

Chymotrypsin cleaves at carboxyl side of $Y$.

Carboxypeptidase $A$ cleaves at amino side of the $C$-terminal amino acid (except $R$ and $K$) of the peptide.

The correct amino acid sequence of the peptide is

  1. $\text{RSYRVA}$
  2. $\text{AVRYSR}$
  3. $\text{SRYVAR}$
  4. $\text{SVRRYA}$
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