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Q43 The molar extinction coefcients of Trp and Tyr at 280 nm are 5690 and 1280 .\f1.cm1,respectively. The polypeptide chain of yeast alcohol dehydrogenase (37 kDa) contains 5 Trp and 14Tyr residues. The absorbance at 280 nm of a 0.32 mgml.l solution of yeast alcohol dehydrogenasemeasured in a cuyette of 1 cm pathlength ill be(Assume that the molar extinction coefficient values for Trp and T31 apply to these amino acids inthe yeast alcohol dehydrogenase).

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