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GATE2016-43

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The molar extinction coeffcients of Trp and Tyr at $280$ nm are $5690$ and $1280 \: M^{-1} \cdot cm^{-1}$, respectively. The polypeptide chain of yeast alcohol dehydrogenase ($37$ kDa) contains $5$ Trp and $14$ Tyr residues. The absorbance at $280$ nm of a $0.32 \text{ mg . mL}^{-1}$ solution of yeast alcohol dehydrogenase measured in a cuvette of $1$ cm pathlength will be _________

(Assume that the molar extinction coefficient values for Trp and Tyr apply to these amino acids inthe yeast alcohol dehydrogenase).

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