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Recent questions tagged enzyme

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GATE BT 2026 | Question: 6
Which of the following enzyme is involved in the protection of cells from reactive oxygen species?HexokinaseGlucose $6$-phosphate dehydrogenaseEnolasePyruvate kinase
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GATE BT 2026 | Question: 11
The catalytic efficiency of an enzyme following Michaelis-Menten kinetics is defined by$k_{\text {Cat }}$$V_{\max } / k_{\mathrm{Cat}}$$k_{\text {Cat }} / K_{\mathrm{M}}$...
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GATE BT 2026 | Question: 22
You are characterizing a new enzyme isolated and purified in the laboratory. If the maximum velocity of the enzyme is $1800 ~\mu$ moles $\mathrm{L}^{-1} \mathrm{~min}^{-1...
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GATE BT 2026 | Question: 25
You have purified an enzyme using a series of chromatographic methods. It was observed that a $10 ~\mu \mathrm{g} \mathrm{~mL}^{-1}$ of this purified enzyme converted $10...
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GATE BT 2026 | Question: 40
An enzyme-catalyzed reaction is found to have $\Delta \mathrm{G}=-100 \mathrm{~kJ} \mathrm{~mol}^{-1}$. Which of the following statements about this reaction is/are true?...
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GATE BT 2025 | Question: 7
​​​​Which one of the following coenzymes is utilised by alanine racemase for the conversion of $L$-Alanine to $D$-Alanine?Pyridoxal phosphateThiamine pyrophosphateTetrahy...
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GATE BT 2025 | Question: 12
​​​​Which of the following enzymes is/are involved in the biogenesis of miRNA?DroshaCas$9$XRCC$4$Dicer
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GATE BT 2025 | Question: 30
Correctly match the Enzyme with its respective Function.\begin{array}{|l|l|}\hline \textbf{ Enzyme } & \textbf{ Function } \\\hline \text{P. Gyrase} & 1. \text{Removes a ...
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GATE BT 2025 | Question: 31
​​​​Correctly match the Coenzyme with its respective involvement in a specific Reaction type.\begin{array}{|l|l|}\hline \textbf{Coenzyme } & \textbf{Reaction type} \\\hli...
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GATE BT 2024 | Question: 27
Match the industrial enzyme (Column I) with its application (Column II).\begin{array}{|c|c|c|}\hline \text{Column I} & \text{Column II} \\\hline \text{P. Lipase} & 1. \...
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GATE BT 2024 | Question: 28
Match the enzyme (Column I) with its corresponding function (Column II).\begin{array}{|c|c|c|}\hline & \text{Column I} & \text{Column II} \\\hline & \text{P. Primase} & 1...
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GATE BT 2024 | Question: 34
Which of the following statements is/are correct about an uncompetitive inhibitor of an enzyme?It binds to the substrate binding site of the enzyme onlyIt binds to the en...
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GATE BT 2023 | Question: 44
An enzyme (E) catalyzes the biochemical reaction $A \rightarrow B$ with $k_{c a t}$ equal to $500 s^{-1}$. If the initial reaction velocity $\left(V_{0}\right)$ is $10 \m...
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GATE BT 2022 | Question: 9
Which of the following statements about reversible enzyme inhibitors are $\text{CORRECT}?$Uncompetitive inhibitors bind only to the enzyme-substrate complexNon-competitiv...
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GATE BT 2022 | Question: 38
Which of the following statements are $\text{CORRECT}$ for an enzyme entrapped in a spherical particle?Effectiveness factor is ratio of the reaction rate with diffusion-l...
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GATE BT 2022 | Question: 40
Which of the following plot(s) is(are) $\text{CORRECT}$ for an enzyme that obeys Michaelis-Menten kinetics, assuming $[S] \ll K_{m}$?$[S]$ is the concentration of the sub...
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GATE BT 2022 | Question: 49
An enzyme catalyzes the conversion of substrate $\text{A}$ into product $\text{B}.$ The rate equation for this reaction is$-r_{A}= \dfrac{\text{C}_{A}}{5+C_{A}} \text{mol...
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GATE BT 2021 | Question: 25
The enzyme $\alpha$-amylase used in starch hydrolysis has an affinity constant $(K_m)$ value of $0.005 \: M$. To achieve one-fourth of the maximum rate of hydrolysis, the...
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GATE2019: 25
Protein concentration of a crude enzyme preparation was $10\:mg\:mL^{-1}$. $10\:\mu L$ of this sample gave an activity of $5\:\mu mol\:min^{-1}$ under standard assay cond...
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GATE2020: 39
Which of the following strategies are used by cells for metabolic regulation?Phosphorylation-dephosphorylationAllosteryFeedback inhibition$P$ and $Q$ only$P$ and $R$ only...
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GATE2020: 12
Which of the following types of molecules act as biological catalysts?Protein$RNA$Phospholipid$P$ and $Q$ only$P$ and $R$ only $Q$ and $R$ only$P, Q$ and $R$
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GATE2019: 5
Which one of the following enzymes is encoded by human immunodeficiency virus $(HIV)$ genome?Reverse transcriptasePhospholipasePhosphatase$ATP$ synthase
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GATE2019: 14
Which one of the following statements is $CORRECT$ for enzyme catalyzed reactions? ( $\Delta G$ is Gibbs free energy change, $K_{eq}$ is equilibrium constant)Enzymes affe...
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GATE2016-14
Which one of the following statements is NOT true?In competitive inhibition, substrate and inhibitor compete for the same active site of an enzymeAddition of a large amou...
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GATE2016-42
In an assay of the type II dehydroquinase of molecular mass $18$ kDa, it is found that the $V_{\text{max}}$ of the enzyme is $0.0134 \: \mu \text{ mol} \cdot \text{min}^{...
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GATE2016-50
An enzyme converts substrate $A$ to product $B$. At a given liquid feed stream of o rate $25$ L.min$^{-1}$ and feed substrate concentration of $2$ mol.L$^{-1}$, the volum...
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GATE2016-52
An enzyme is immobilized on the surface of a non-porous spherical particle of $2$ mm diameter. The immobilized enzyme is suspended in a solution having bulk substrate con...
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GATE BT 2017 | Question: 1
An enzyme catalyzes a reaction bydecreasing the energy of the substrate.decreasing the activation energy of the reaction.decreasing product stability.increasing the activ...
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GATE BT 2017 | Question: 21
An enzymatic reaction exhibits Michaelis-Menten kinetics. For this reaction, on doubling the concentration of enzyme while maintaining $[S]>>[E_0]$both $K_m$ and $V_{\tex...
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GATE BT 2017 | Question: 35
The specific activity of an enzyme in a crude extract of $\textit{E.coli}$ is $9.5$ units/mg of protein. The specific activity increased to $68$ units/mg of protein upon ...
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GATE BT 2015 | Question: 19
A single subunit enzyme converts $420$ $\mu$moles of substrate to product in one minute. The activity of the enzyme is ________ $\times 10^{-6}$ Kalal.
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GATE BT 2015 | Question: 31
Which one of the following graphs represents uncompetitive inhibition?
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GATE BT 2015 | Question: 54
The $K_i$ of a novel competitive inhibitor designed against an enzyme is $2.5 \: \mu M$. The enzyme was assayed in the absence or presence of the inhibitor $(5 \: \mu M)$...
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GATE BT 2013 | Question: 34
The activity of an enzyme was measured by varying the concentration of the substrate $(S)$ in the presence of three different concentrations of inhibitor $\text{(I) 0, 2 ...
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GATE BT 2013 | Question: 33
Match the herbicides in $\text{Group I}$ with the target enzymes in $\text{Group II}$ $\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \hline P. & ...
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GATE BT 2013 | Question: 13
The catalytic efficiency for an enzyme is defined as$K_{cat}$$\frac{V_{max}}{K_{cat}}$$\frac{k_{cat}}{K_m}$$\frac{K_{cat}}{V_{max}}$
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GATE BT 2012 | Question: 55
$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and...
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GATE BT 2012 | Question: 51
Answer the question based on the information given below:The purification data for an enzyme is given below:$\begin{array}{|l|l|l|l|l|l|} \hline & \text{Step} & \text{Vol...
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GATE BT 2012 | Question: 50
Answer the question based on the information given below:The purification data for an enzyme is given below:$\begin{array}{|l|l|l|l|l|l|} \hline & \text{Step} & \text{Vol...
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GATE BT 2012 | Question: 46
An enzymatic reaction is described by the following rate expression.$$v = \frac{v_mS}{K_m+S+S^2/K_s},$$Which one of the following curves represents this expression?
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