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An enzyme is immobilized on the surface of a non-porous spherical particle of 2 mm diameter. Theimmobilized enzx me is suspended in a solution hat ing bulk substrate concentration of 10 m.\L Theenzw me follous rst order kinetics mth rate constant 10 s and the external mass transfercoefficient is 1 cm 5 Assume steadV state condition \\ herein rate of enz3me reaction (mmol L s )at the surface is equal to mass transfer rate (mmol L s ) The substrate concentration at the surfaceof the immobilized particle ill be mM.

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