0 0 votes $\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and a voidage ($\epsilon$) of $0.55$. The $K’_m$ and $V’_m$; for the immobilized enzyme are $0.72 \: gl^{-1}$ and $18 \: gl^{-1}h^{-1}$, respectively. The lactose concentration in the field stream is $20 \: gl^{-1}$, and a fractional conversion of $0.90$ is desired. Diffusional limitations may be ignored. The feed flow rate required for the above bioconversion will be $50 \: lh^{-1}$ $55 \: lh^{-1}$ $137 \: lh^{-1}$ $550 \: lh^{-1}$ Bioreaction Engineering gate2012 bioreaction-engineering enzyme biochemistry numerical-answers + – Milicevic3306 7.9k points answer comment Share Follow 0 reply Please log in or register to add a comment.
