Recent questions tagged analytical-techniques

Cancer cells display a high level of heterogeneity in the expression of TP$53$ protein. Which of the following techniques can be directly used to determine the intratumor...

Which one of the following proteins would elute third in gel filtration chromatography from a mixture of four proteins given below?Insulin $(6 \text{ kDa})$Myoglobin $(17...

BLAST and its extensions can be used to determine similarity of a query protein sequence with which of the following database(s)?A database of translated nucleotide seque...

You have purified an enzyme using a series of chromatographic methods. It was observed that a $10 ~\mu \mathrm{g} \mathrm{~mL}^{-1}$ of this purified enzyme converted $10...

​​​​​​Which of the following separation processes is/are based on molecular size?Size-exclusion chromatographyIon exchange chromatographyMembrane ultrafiltrationUltracent...

​​Which of the following show(s) optical activity at $100 \: \mathrm{mM}$ concentration in water?Solution of NaClSolution of D-GlucoseSolution of GlycineSolution of L-Pro...

​​​​Correctly match the following Bioinformatic tool/Database with its respective Utility.\begin{array}{|l|l|}\hline \textbf{Bioinformatic tool/Database} & \textbf{Utilit...

​​​​Which of the following methods is/are used for identifying histone modifications?ChIP-seqMass spectrometryImmunofluorescencePatch-clamp electrophysiology

The percentage of light that would pass through a sample with an absorbance of $2$ would be $\_\_\_\_\_$ $\%$ .(Round off to the nearest integer)

An NMR spectrometer operating at proton resonance frequency $(v)$ of $1 \: \mathrm{GHz}$ will have a magnetic field strength of $\_\_\_\_$ Tesla (T).The gyromagnetic rati...

In adsorption chromatography, the adsorption of uncharged solute molecules onto a silica-based stationary phase is by $\_\_\_\_\_\_\_$.covalent bondselectrostatic interac...

Match the chemical (Column I) with its use (Column II).\begin{array}{|c|c|c|}\hline \text{Column I} & \text{Column II} \\\hline \text{P. Diethylpyrocarbonate} & 1. \text{...

Match the item in Column I with the corresponding technique in Column II.\begin{array}{|c|c|c|}\hline & \text{Column I} & \text{Column II} \\\hline & \text{P. Blue laser}...

In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?P. Alkaline phosphataseQ. TrypsinaseR. Horseradish peroxidaseS. Amylase...

Which of the following methods are used for detection of DNA and RNA, respectively?Southern and Northern blotting Southern and Western blottingNorthern and Southern blott...

Among individuals in a human population, minor variations exist in nucleotide sequences of chromosomes. These variations can lead to gain or loss of sites for specific re...

Match the stationary phase (Column I) with its corresponding chromatography technique (Column II).$$\begin{array} {lll} \textbf{Column I} & & \textbf{Column II} \\ \text{...

Which of the following spectroscopic technique(s) can be used to identify all the functional groups of an antibiotic contaminant in food?InfraredCircular dichroismNuclear...

Which one of the following techniques/tools is $\text{NOT}$ used for inserting a foreign gene into a cell?$\text{DNA}$ microarrayElectroporationGene gunMicroinjection

Match separation methods in $\text{Group I}$ with associated properties in $\text{Group II}$.$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \textbf{Group II} \\ ...

Tertiary structure of a protein consisting of $\alpha$-helices and $\beta$-strands can be determined bycircular dichroism spectroscopymass spectrometrynuclear magnetic re...

Which of the following nucleus/nuclei is/are $\text{NMR}$ active?$^1H$$^{13}C$$^{16}O$$^{32}S$

A $\text{DNA}$ solution of $50 \: \mu g \: mL^{-1}$ concentration gives an absorbance of $1.0$ at $260 \textit{ nm}$. An aliquot of $20 \: \mu L$ from a $50 \: \mu L$ pur...

For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated $DNA$?$KpnI$$DpnI$$XhoI$$MluI$

$Group\; I$ lists spectroscopic methods and $Group\; II$ lists biomolecular applications of these methods. Match the methods in $Group\; I$ with the applications in $Grou...

The hexapeptide $P$ has an isoelectric point $(pI)$ of $6.9$. Hexapeptide $Q$ is a variant of $P$ that contains valine instead of glutamate at position $3$. The two pepti...

A UV-visible spectrophotometer has a minimum detectable absorbance of $0.02$. The minimum concentration of a protein sample that can be measured reliably in this instrume...

The $DNA$ sequence shown below is to be amplified by $PCR$:$5{}'$ GCTAAGATCTGAATTTTCC$\dots\dots$TTGGGCAATAATGTAGCGC$3{}'$$3{}'$ CGATTCTAGACTTAAAAGG$\dots\dots$AACCCGTTAT...

Protein $A$ and protein $B$ form a covalent complex. Gel filtration chromatography of this complex showed a peak corresponding to $200 \:kDa$. $SDS-PAGE$ analysis of this...

Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are $5.6$ and $6.8$. Which one of the following techn...

Which one of the following techniques can be used to compare the expression of a large number of genes in two biological samples in a single experiment?Polymerase chain r...

Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, $\rho$ of a double stranded $Cs^+DNA$ is given ...

Which one of the following is NOT used for the measurement of cell Viability in animal cell culture?Trypan blue dye exclusionTetrazolium (MTT) assayLDH activity in the cu...

Which one of the following techniques relies on the spin angular momentum of a photon?CD spectroscopyFluorescence spectroscopyIR spectroscopyRaman spectroscopy

A $1.2$ kb DNA fragment was cloned into $\textit{Bam}$HI and $\textit{Eco}$RI sites located on a $2.8$ kb cloning vector. The $\textit{Bam}$HI and $\textit{Eco}$RI sites ...

The activity of lactate dehydrogenase can be measured by monitoring the following reaction: $$\text{Pyruvate} + \text{NADH} \rightarrow \text{Lactate} + \text{NAD}^+$$ Th...

Analysis of a hexapeptide using enzymatic cleavage reveals the following result:Amino acid composition of the peptide is: $2R, A, V, S, Y$Trypsin digestion yields two fra...

A protein is to be purified using ion-exchange column chromatography. The relationship between HETP (Height Equivalent to Theoretical Plate) and the linear liquid velocit...