Recent questions tagged analytical-techniques

In a thin layer chromatography experiment using a silica gel plate, a compound showed migration of $12.5$ cm and the solvent front showed migration of $18$ cm. The $R_f$ ...

Which one of the following techniques can be used to determine the structure of a $15$ kDa globular protein at atomic resolution?Raman spectroscopyIR spectroscopyUV spect...

Enzyme-linked immunosorbent assay (ELISA) is used forquantifying antibody levels in blooddetermining the molecular weight of an antigenpurifying proteins from biological ...

A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication?Northern...

Which one of the following graphs represents the kinetics of protein precipitation by addition of ammonium sulphate? On the $Y$-axis, [Protein] represents the concentrati...

The circular dichroism spectra of three proteins $P$, $Q$, and $R$ are given below:Choose the correct combination.$P: \alpha- \text{helix}, Q: \beta- \text{sheet}, \text{...

Shown below id a plasmid vector (P) and an insert (Q). The insert was cloned into the BamHI site of the vector. The recombinant plasmid was isolated and digested with Bam...

Which one of the following organisms is used for the determination of phenol coefficient of a disinfectant?Salmonella typhiEscherichia coliCandida albicansBacillus psychr...

Which one of the following graphs represents uncompetitive inhibition?

The interaction between an antigen $(Ag)$ and a single-chain antibody $(Ab)$ was studied using Scatchard analysis. The result is shown below.The affinity of interaction a...

Match the reagents in $\text{Group I}$ with their preferred cleavage sites in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \...

If the dissociation constant for solute-adsorbent binding is $K_D$, the retention time of the solute in a chromatography columnincreases with increasing $K_D$decreases wi...

The plasmid DNA was subjected to restriction digestion using the enzyme $\textit{EcoRI}$ and analysed on an agarose gel. Assuming digestion has worked (the enzyme was act...

The activity of an enzyme was measured by varying the concentration of the substrate $(S)$ in the presence of three different concentrations of inhibitor $\text{(I) 0, 2 ...

Match the entries in the $\text{Group I}$ with the elution conditions in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \hline...

Answer the question based on the information given below:The purification data for an enzyme is given below:$\begin{array}{|l|l|l|l|l|l|} \hline & \text{Step} & \text{Vol...

Match the entries in $\text{Group I}$ with the process parameters in $\text{Group II}$.$\begin{array}{|C|l|C|l|} \hline &\textbf{Group I} & {} & \text{Group II} \\\hline ...

The helix content of a protein can be determined usingan infrared spectrometera fluorescence spectrometera circular dichroism spectrometera UV-Visible spectrophotometer

In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen withferrous ammonium sulfateuranium acetatesodium chloridebasic fuchsin

Protein-DNA interactions $\text{in vivo}$ can be studied bygel shift assaySouthern hybridizationchromatin immunoprecipitation assayfluorescence $\text{in situ}$ hybridiza...