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Match the entries in the $\text{Group I}$ with the elution conditions in $\text{Group II}$.

$\begin{array}{|l|l|l|l|} \hline & \text{Group I} &&  \text{Group II} \\ \hline P. & \text{Ion-exchange chromatography} & 1.  & \text{Isocratic solvent} \\ \hline Q. & \text{Hydrophobic column chromatography} &  2.  & \text{Ampholytes} \\ \hline R. & \text{Gel filtration chromatography} &  3. & \text{Increasing gradient of salt} \\ \hline S. & \text{Chromatofocusing} &  4. & \text{Decreasing gradient of polarity} \\ \hline  \end{array}$

  1. $\text{P-4, Q-1, R-2, S-3}$
  2. $\text{P-4, Q-3, R-1, S-2}$
  3. $\text{P-3, Q-4, R-1, S-2}$
  4. $\text{P-3, Q-4, R-2, S-1}$

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