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Cancer cells display a high level of heterogeneity in the expression of TP$53$ protein. Which of the following techniques can be directly used to determine the intratumoral heterogeneity of TP$53$ expression in a given tumor sample, without resorting to additional cell separation techniques?

  1. Immunoblotting
  2. Flow cytometry
  3. ELISA
  4. Quantitative RT-PCR

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