0 0 votes If the dissociation constant for solute-adsorbent binding is $K_D$, the retention time of the solute in a chromatography column increases with increasing $K_D$ decreases with increasing $K_D$ passes through minimum with increasing $K_D$ is independent of $K_D$ Biochemistry gate2014 analytical-techniques biochemistry + – Milicevic3306 7.9k points answer comment Share Follow 0 reply Please log in or register to add a comment.