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If the dissociation constant for solute-adsorbent binding is $K_D$, the retention time of the solute in a chromatography column

  1. increases with increasing $K_D$
  2. decreases with increasing $K_D$
  3. passes through minimum with increasing $K_D$
  4. is independent of $K_D$
in Biochemistry 7.9k points
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