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Q51 A protein is to be puried using ion-exchange column chromatography. The relationship betweenHETP (Height Equivalent to Theoretical Plate) and the linear liquid velocity of mobile phase isgiven by:H = i + Bu + CuWhere H is HETP (m) and u is linear liquid velocity of mobile phase (ms'l). The values of A, B andC are 3 XIO'S m2.s1, 3 s and 6X10" m_ respectively. The number of theoretical plates based onminimum HETP for a column of 66 cm length ill be

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