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Which one of the following methods CANNOT be used to determine the secondary structure content of a protein?

  1. Circular dichroism spectroscopy
  2. Fourier transform infrared spectroscopy
  3. Mass spectrometry
  4. X-ray crystallography

     

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Mass spectrometry can't be used to determine secondary structure content of protein as MS can't able to trace back the origin of peptides.

 

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