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In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?

P. Alkaline phosphatase
Q. Trypsinase
R. Horseradish peroxidase
S. Amylase

  1. $\mathrm{P}$ and $\mathrm{R}$
  2. $\text{P}$ and $\text{Q}$
  3. $\text{Q}$ and $\text{S}$
  4. $\mathrm{R}$ and $\mathrm{S}$

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